Immune Characteristics of LYN in Tumor Microenvironment of Gliomas.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35186945", "kind": "science-record", "revision": 5, "content_hash": "sha256:7a44945d65d438724d22f125398aa823f2c4adab7d10034cfd35577dba06f1c9", "created_at": "2026-11-01T17:36:57Z", "updated_at": "2026-11-01T22:05:56Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Immune Characteristics of LYN in Tumor Microenvironment of Gliomas.", "doi": "10.3389/fcell.2021.760929", "pmid": "35186945", "authors": ["Chonghua Jiang", "Hao Zhang", "Wantao Wu", "Zeyu Wang", "Ziyu Dai", "Liyang Zhang", "Zhixiong Liu", "Quan Cheng"], "journal": "Frontiers in cell and developmental biology", "year": 2021, "abstract": "The prognosis of gliomas is poor and there are limited therapeutic approaches. Immunotherapy has become a promising treatment for gliomas. Here, we explored the expression pattern of Lck/yes-related protein tyrosine kinase (LYN) in gliomas and assessed its value as an immunotherapy biomarker. Transcriptional data was mined from two publicly available datasets, TCGA and CGGA, and used to investigate the correlation between LYN and clinical characteristics including patient prognosis, somatic mutation, and immune infiltrating features in gliomas. Besides, the correlation between LYN and classical immune checkpoint molecules was explored. Glioma samples obtained from the Xiangya Hospital cohort were used for immunohistochemistry staining. High expression level of LYN was observed in advanced gliomas and other cancer types, which predicted a worse prognosis. LYN stratified patients' survival in the Xiangya cohort and was also significantly associated with infiltrating immune cell types and inflammatory activities in the tumor microenvironment. LYN was involved in tumor mutation, correlated with the regulation of oncogenic genes, and also showed a significant positive correlation with PD-L1. LYN can be a potential diagnostic marker and immunotherapy marker in gliomas.", "claims": [{"id": "claim-method", "statement": "R package WGCNA was applied to explore the LYN related genes.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35186945", "locator": "sections[15] \"Weighted Gene Co-expression Network Analysis\" paragraph 2", "quote": "R package WGCNA was applied to explore the LYN related genes."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding authors.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35186945", "locator": "sections[18] \"Data Availability Statement\" paragraph 2", "quote": "The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding authors."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Notably, the brown module exhibited the highest correlation coefficient with a high LYN expression level (Figure 9B, r = 0.7, p < 8e-102).", "kind": "quantitative", "target": {"value": 0.7, "unit": "correlation coefficient", "tolerance": null, "source": "Notably, the brown module exhibited the highest correlation coefficient with a high LYN expression level (Figure 9B, r = 0.7, p < 8e-102)."}, "evidence": [{"asset": "source:pmid:35186945", "locator": "sections[8] \"Identification of Gene Modules Associated With LYN Expression\" paragraph 2", "quote": "Notably, the brown module exhibited the highest correlation coefficient with a high LYN expression level (Figure 9B, r = 0.7, p < 8e-102)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "Notably, the brown module exhibited the highest correlation coefficient with a high LYN expression level (Figure 9B, r = 0.7, p < 8e-102).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35186945"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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