LINC00665 regulates hepatocellular carcinoma by modulating mRNA via the m6A enzyme.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35233461", "kind": "science-record", "revision": 4, "content_hash": "sha256:040c1c017a75c91d8f9e5c00a8cabcba64029bf9f70704fcafae92c1f14d11bc", "created_at": "2026-11-01T17:36:59Z", "updated_at": "2026-11-01T22:06:00Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "LINC00665 regulates hepatocellular carcinoma by modulating mRNA via the m6A enzyme.", "doi": "10.1515/biol-2022-0003", "pmid": "35233461", "authors": ["Ming Lei", "Xinghua Du", "Xiaokai Li", "Fuke Wang", "Ling Gu", "Feng Guo"], "journal": "Open life sciences", "year": 2022, "abstract": "This study aimed to reveal the mechanism by which long noncoding RNAs (lncRNAs) modulate hepatocellular carcinoma (HCC) by regulating mRNA via the N6-methyladenosine (m6A) enzyme. The expression and clinical data of 365 HCC patients and 50 healthy control samples were downloaded from the the Cancer Genome Atlas (TCGA) database. Differentially expressed lncRNAs (DElncRNAs) and differentially expressed mRNAs (DEmRNAs) screened using limma packages from the R. m6A2Target database were used to predict the relationship between m6A enzyme-lncRNA and m6A enzyme-mRNA. The mRNAs in the lncRNA-m6A enzyme-mRNA network were subjected to enrichment analysis. Cox regression analysis was used to screen for RNAs significantly related to HCC prognosis. The expression of differentially expressed RNAs (DERs) was verified using the TCGA dataset and GSE55092. Eighty-five DElncRNAs and 747 DEmRNAs were identified. The mRNAs in the lncRNA-m6A enzyme-mRNA network were primarily involved in mitotic cell division, the p53 signaling pathway, and the cell cycle. Three lncRNAs and 14 mRNAs were significantly associated with HCC prognosis. Furthermore, the expression of 12 DERs differed significantly between patients in the early and advanced stages. LINC00665 was predicted to regulate 11 mRNAs by modulating IGF2BP1, IGF2BP2, and YTHDF1. Thus, this study provides new insights into the roles of lncRNA and m6A enzymes in HCC.", "claims": [{"id": "claim-method", "statement": "Univariate and multivariate Cox regression analyses in the survival package (version 2.41-1) [20] of R3.6.1 were used to screen for significantly independent prognostic lncRNAs and mRNAs.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35233461", "locator": "sections[6] \"Prognostic correlation analysis of lncRNAs and mRNAs in the comprehensive network\" paragraph 2", "quote": "Univariate and multivariate Cox regression analyses in the survival package (version 2.41-1) [20] of R3.6.1 were used to screen for significantly independent prognostic lncRNAs and mRNAs."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data availability statement: The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35233461", "locator": "sections[16] \"fn-group\" paragraph 1", "quote": "Data availability statement: The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "LINC00665 was predicted to regulate 11 mRNAs by modulating IGF2BP1, IGF2BP2, and YTHDF1.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35233461", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "LINC00665 was predicted to regulate 11 mRNAs by modulating IGF2BP1, IGF2BP2, and YTHDF1."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "LINC00665 was predicted to regulate 11 mRNAs by modulating IGF2BP1, IGF2BP2, and YTHDF1.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35233461"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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