Potential Role of the Chemotaxis System in Formation and Progression of Intracranial Aneurysms Through Weighted Gene Co-Expression Network Analysis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35250300", "kind": "science-record", "revision": 4, "content_hash": "sha256:d1929b26ad756760f535dd904f4830f1d10f992b1716c1ce6c038934409c24c8", "created_at": "2026-11-01T17:37:00Z", "updated_at": "2026-11-01T22:06:02Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Potential Role of the Chemotaxis System in Formation and Progression of Intracranial Aneurysms Through Weighted Gene Co-Expression Network Analysis.", "doi": "10.2147/IJGM.S347420", "pmid": "35250300", "authors": ["Huaxin Zhu", "Jiacong Tan", "Yeyu Zhao", "Zhihua Wang", "Zhiwu Wu", "Meihua Li"], "journal": "International journal of general medicine", "year": 2022, "abstract": "BACKGROUND: Intracranial aneurysm (IA) is the most common and is the main cause of spontaneous subarachnoid hemorrhage (SAH). The underlying molecular mechanisms for preventing IA progression have not been fully identified. Our research aimed to identify the key genes and critical pathways of IA through gene co-expression networks.\nMETHODS: Gene Expression Omnibus (GEO) datasets GSE13353, GSE54083 and GSE75436 were used in the study. The genetic data were analyzed by weighted gene co-expression network analysis (WGCNA). Then the clinically significant modules were identified and the differentially expressed genes (DEGs) with the genes were intersected in these modules. GO (gene ontology) and KEGG (Kyoto Gene and Genomic Encyclopedia) were used for gene enrichment analysis to determine the function or pathway. In addition, the composition of immune cells was analyzed by CIBERSORT algorithm. Finally, the hub genes and key genes were identified by GSE122897.\nRESULTS: A total of 266 DEGs and two modules with clinical significance were identified. The inflammatory response and immune response were identified by GO and KEGG. CCR5, CCL4, CCL20, and FPR3 were the key genes in the module correlated with IA. The proportions of infiltrating immune cells in IA and normal tissues were different, especially in terms of macrophages and mast cells.\nCONCLUSION: The chemotactic system has been identified as a key pathway of IA, and interacting macrophages may regulate this pathological process.", "claims": [{"id": "claim-method", "statement": "The genetic data were analyzed by weighted gene co-expression network analysis (WGCNA).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35250300", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "The genetic data were analyzed by weighted gene co-expression network analysis (WGCNA)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All datasets of this study are available in the GEO database (https://www.ncbi.nlm.nih.gov/geo/, accessed on 10 July 2021).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35250300", "locator": "sections[21] \"Data Sharing Statement\" paragraph 2", "quote": "All datasets of this study are available in the GEO database (https://www.ncbi.nlm.nih.gov/geo/, accessed on 10 July 2021)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "A total of 266 DEGs and two modules with clinical significance were identified.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35250300", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "A total of 266 DEGs and two modules with clinical significance were identified."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "A total of 266 DEGs and two modules with clinical significance were identified.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35250300"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE122897"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE122897"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:35250300", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.2147/IJGM.S347420", "sha256": 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