Circ_0000228 Promotes Cervical Cancer Progression via Regulating miR-337-3p /TGFBR1 Axis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35279965", "kind": "science-record", "revision": 5, "content_hash": "sha256:96d58af084bafc29d902c398347b2b2b9930afef42e9095cb7a7df096d6cf1f8", "created_at": "2026-11-01T18:14:03Z", "updated_at": "2026-11-01T22:48:38Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Circ_0000228 Promotes Cervical Cancer Progression via Regulating miR-337-3p /TGFBR1 Axis.", "doi": "10.22074/cellj.2022.7914", "pmid": "35279965", "authors": ["Yongqian Xu", "Xiaona Dong", "Baoli Ma", "Pingping Mu", "Xiang Kong", "Dongmei Li"], "journal": "Cell journal", "year": 2022, "abstract": "Objective: This study aims to investigate the biological function of circular RNA (circRNA) circ_0000228 in the cervical cancer (CC).\nMaterials and Methods: In this experimental study, the GSE113696 dataset was downloaded from the Gene Expression Omnibus (GEO). GEO2R was employed to obtain differentially expressed circRNA between CC tissues and matched paracancerous tissues. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were employed to detect circ_0000228, microRNA-337-3p (miR-337-3p ) and transforming growth factor, beta receptor I (TGFBR1) expression levels in the CC tissues and cells. Following gain-of-function and loss-of-function models establishment, CCK-8 and BrdU tests were conducted to examine cell proliferation. Transwell experiment was executed to examine CC cells migration and invasion. A lung metastasis model was utilized to determine the ability of circ_0000228 on the lung metastasis. Bioinformatics analysis, dual-luciferase reporter experiment and RNA immunoprecipitation (RIP) assay were applied to verify the targeting relationship among miR-337-3p , circ_0000228, and TGFBR1.\nResults: Circ_0000228 expression in the CC tissues and cells was up-modulated. Circ_0000228 overexpression markedly enhanced cell proliferation, migration, and invasion, while knocking down circ_0000228 remarkably repressed cell proliferation, migration, and invasion. MiR-337-3p could be adsorbed by circ_0000228. TGFBR1 was identified as a target gene of miR-337-3p that indirectly and positively modulated bycirc_0000228 in the CC cells.\nConclusion: Circ_0000228 up-modulates TGFBR1 by targeting miR-337-3p to enhance CC cell proliferation, migration and invasion. Also, Circ_0000228 is a promising therapeutic target for the CC.", "claims": [{"id": "claim-method", "statement": "Also, t test was adopted for comparison between two groups, and one-way ANOVA was used for comparison of the means among multiple groups.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35279965", "locator": "sections[14] \"Statistical analysis\" paragraph 2", "quote": "Also, t test was adopted for comparison between two groups, and one-way ANOVA was used for comparison of the means among multiple groups."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Circ_0000228 up-modulates TGFBR1 by targeting miR-337-3p to enhance CC cell proliferation, migration and invasion.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35279965", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "Circ_0000228 up-modulates TGFBR1 by targeting miR-337-3p to enhance CC cell proliferation, migration and invasion."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "Circ_0000228 up-modulates TGFBR1 by targeting miR-337-3p to enhance CC cell proliferation, migration and invasion.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35279965"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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