Identification of ITGAX and CCR1 as potential biomarkers of atherosclerosis via Gene Set Enrichment Analysis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35287505", "kind": "science-record", "revision": 4, "content_hash": "sha256:205b9b7aa056098ff7bc8aa54d09dd1e7fc7a7b9ce47c3721a4ba14043eabeb1", "created_at": "2026-11-01T18:14:04Z", "updated_at": "2026-11-01T22:06:06Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification of ITGAX and CCR1 as potential biomarkers of atherosclerosis via Gene Set Enrichment Analysis.", "doi": "10.1177/03000605211039480", "pmid": "35287505", "authors": ["Sheng Yan", "Lingbing Meng", "Xiaoyong Guo", "Zuoguan Chen", "Yuanmeng Zhang", "Yongjun Li"], "journal": "The Journal of international medical research", "year": 2022, "abstract": "OBJECTIVE: Atherosclerosis (AS) is a life-threatening disease in aging populations worldwide. However, the molecular and gene regulation mechanisms of AS are still unclear. This study aimed to identify gene expression differences between atheroma plaques and normal tissues in humans.\nMETHODS: The expression profiling dataset GSE43292 was obtained from the Gene Expression Omnibus (GEO) dataset. The differentially expressed genes (DEGs) were identified between the atheroma plaques and normal tissues via GEO2R, and functional annotation of the DEGs was performed by GSEA. STRING and MCODE plug-in of Cytoscape were used to construct a protein-protein interaction (PPI) network and analyze hub genes. Finally, quantitative polymerase chain reaction (qPCR) was performed to verify the hub genes.\nRESULTS: Overall, 134 DEGs were screened. Functional annotation demonstrated that these DEGs were mainly enriched in sphingolipid metabolism, apoptosis, lysosome, and more. Six hub genes were identified from the PPI network: ITGAX, CCR1, IL1RN, CXCL10, CD163, and MMP9. qPCR analysis suggested that the relative expression levels of the six hub genes were significantly higher in AS samples.\nCONCLUSIONS: We used bioinformatics to identify six hub genes: ITGAX, CCR1, IL1RN, CXCL10, CD163, and MMP9. These hub genes are potential promising diagnostic and therapeutic targets for AS.", "claims": [{"id": "claim-method", "statement": "The DEGs were identified between the atheroma plaques and normal tissue via GEO2R.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35287505", "locator": "sections[4] \"Identification of DEGs with GEO2R\" paragraph 2", "quote": "The DEGs were identified between the atheroma plaques and normal tissue via GEO2R."}], "state": "complete"}, {"id": "claim-data-access", "statement": "GSE43292, an expression profiling dataset, was downloaded from the GEO database, which was sequenced on the platform GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35287505", "locator": "sections[3] \"Access to public data\" paragraph 2", "quote": "GSE43292, an expression profiling dataset, was downloaded from the GEO database, which was sequenced on the platform GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Overall, 134 DEGs were screened.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35287505", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "Overall, 134 DEGs were screened."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Overall, 134 DEGs were screened.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35287505"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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