Identification of Immune Markers in Dilated Cardiomyopathies with Heart Failure by Integrated Weighted Gene Coexpression Network Analysis.

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However, individual differences are so obvious that the clinical effect is not satisfactory. In order to find immune-related biomarkers of DCM to guide treatment and improve clinical efficacy, we downloaded a GSE120895 dataset from the Gene Expression Omnibus (GEO) database using CIBERSORT and WGCNA algorithms in RStudio and visualizing the protein-protein interaction (PPI) network for key modules by Cytoscape, and finally obtained six hub genes. A GSE17800 dataset was downloaded from the GEO dataset to verify the diagnostic values of hub genes, MYG1, FLOT1, and ATG13, which were excellent. Our study revealed unpublished potential immune mechanisms, biomarkers, and therapeutic targets of DCM.", "claims": [{"id": "claim-data-access", "statement": "The data presented in this study are openly available in Gene Expression Omnibus (GEO) (URL: https://www.ncbi.nlm.nih.gov/geo/, accessed on 17 January 2022).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35327947", "locator": "sections[23] \"Data Availability Statement\" paragraph 2", "quote": "The data presented in this study are openly available in Gene Expression Omnibus (GEO) (URL: https://www.ncbi.nlm.nih.gov/geo/, accessed on 17 January 2022)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Using GSE120895 with CIBERSORT and WGCNA, the study identified six hub genes associated with immune-related modules in dilated cardiomyopathy with heart failure.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35327947", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "A GSE17800 dataset was downloaded from the GEO dataset to verify the diagnostic values of hub genes, MYG1, FLOT1, and ATG13, which were excellent."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Using GSE120895 with CIBERSORT and WGCNA, the study identified six hub genes associated with immune-related modules in dilated cardiomyopathy with heart failure.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35327947"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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