miR-143-5p suppresses breast cancer progression by targeting the HIF-1α-related GLUT1 pathway.

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Several microRNAs (miRs), including miR-143-5p, have been reported to be vital for regulating hallmarks of cancer; however, the effect of miR-143-5p on BC requires further exploration. The present study performed bioinformatics analysis on GSE42072 and GSE41922 datasets from the National Center for Biotechnology Information Gene Expression Omnibus (GEO) database to identify miR-143-5p expression patterns. Furthermore, miR-143-5p expression was detected in BC cell lines and tissues via reverse transcription-quantitative PCR. Post-transfection with miR-143-5p mimics, Cell Counting Kit-8, colony formation and Transwell assays were performed to explore the effects of miR-143-5p on BC cell proliferation, colony formation, and migration. The association of miR-143-5p with the hypoxia-inducible factor-1α (HIF-1α)-associated glucose transporter 1 (GLUT1) pathway was explored via western blotting, immunofluorescence and dual-luciferase reporter assay. The present study detected high expression of miR-143-5p in BC tissue of the GSE42072 and serum of the GSE41922 datasets by GEO chip analysis. Additionally, the expression levels of miR-143-5p were decreased in BC tissues compared with those in adjacent healthy tissues, and low miR-143-5p expression was associated with a poorer prognosis and shorter survival time in patients with BC. In vitro, miR-143-5p expression levels were decreased in BC cells, and transfection with miR-143-5p mimics suppressed BC cell proliferation, colony formation, migration. Furthermore, miR-143-5p targeted the HIF-1α-related GLUT1 pathway, and inhibited HIF-1α and GLUT1 expression. Additionally, HIF-1α agonists reversed the miR-143-5p-induced inhibition during tumorigenesis. In conclusion, miR-143-5p exhibited low expression in BC tissues, and suppressed BC cell proliferation, colony formation, migration. Moreover, the antitumor effects of miR-143-5p targeted the HIF-1α-related GLUT1 pathway.", "claims": [{"id": "claim-method", "statement": "The R language ‘limma’ package was used to analyze mRNA with differential expression based on the P<0.001 and |logFC|>2 thresholds.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35350590", "locator": "sections[14] \"Enrichment analysis of differential gene pathways\" paragraph 2", "quote": "The R language ‘limma’ package was used to analyze mRNA with differential expression based on the P<0.001 and |logFC|>2 thresholds."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request. The microarray data are available in Array Express (accession no. 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The detailed patient characteristics are d", "o reverse transcription-quantitative PCR (RT-qPCR). The detailed patient characteristics are d"], "order_basis": "paper_text_order:3131", "id": "assay:5"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["n Type Culture Collection. The BC cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Gi", "osphere containing 5% CO2. MCF10A cells were cultured in DMEM-Nutrient Mixture F-12 supplemented with", "ntaining 5% CO2. BT-549 and MCF-7 cells were cultured in 6-well plates at a density of 5×105 per well", "ial cells, and the human BC cell lines T47D, MCF-7, ZR-75-1, MDA-MB-468, SK-BR-3 and BT-549, we"], "order_basis": "paper_text_order:3472", "id": "assay:6"}, {"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["ession and clinicopathological features. The CCK-8 assay data were compared by two-way analysis"], "order_basis": "paper_text_order:5926", "id": "assay:7"}, {"role": "data_generation", "definition_id": "microarray", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["responding author on reasonable request. The microarray data are available in Array Express (accessi"], "order_basis": "paper_text_order:7306", "id": "assay:8"}, {"role": "analysis", "definition_id": "differential_expression", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["normalise_counts", "differential_expression_fit", "fdr_bh"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["The R language ‘limma’ package was used to analyze mRNA with differential expression based on the P<0.001 and |logFC|>2 thresholds."], "order_basis": "consumes_earlier_output", "id": "assay:9"}]}