Kinetochore-associated protein 1 promotes the invasion and tumorigenicity of cervical cancer cells via matrix metalloproteinase-2 and matrix metalloproteinase-9.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35389773", "kind": "science-record", "revision": 4, "content_hash": "sha256:bdada790435d6aaad6b943312f8ab01d3fbc8dc9cd97ed2e579db874026d2639", "created_at": "2026-11-01T18:14:12Z", "updated_at": "2026-11-01T22:06:15Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Kinetochore-associated protein 1 promotes the invasion and tumorigenicity of cervical cancer cells via matrix metalloproteinase-2 and matrix metalloproteinase-9.", "doi": "10.1080/21655979.2022.2061144", "pmid": "35389773", "authors": ["Caimei Wang", "Yiyuan Wang", "Congrong Liu", "Xiaoyu Meng", "Zhongxia Hang"], "journal": "Bioengineered", "year": 2022, "abstract": "Cervical cancer, a common cancer in women, has become a serious social burden. Kinetochore-associated protein 1 (KNTC1) that regulates the cell cycle by regulating mitosis is related to the malignant behavior of different types of tumors. However, its role in the development of cervical cancer remains unclear. In this study, we initially explored the role of KNTC1 in cervical cancer. KNTC1 expression and relevant information were downloaded from The Cancer Genome Atlas (TCGA) and dataset GSE63514 in the Gene Expression Omnibus (GEO) database for bioinformatics analyses. Cell proliferation was detected by cell counting kit-8 (CCK8) and colony formation assays. Wound healing and Transwell assays were used to evaluate cell migration and invasion abilities. Protein expression levels of matrix metallopeptidase 2 (MMP2) and matrix metallopeptidase 9 (MMP9) were measured by western blotting. Nude mouse models of subcutaneous xenograft tumor were constructed to analyze tumor growth in vivo. CCK8 and colony formation assay results demonstrated that the proliferation rate of SiHa and C-33A cells decreased when KNTC1 was silenced. Western blot and Transwell assays indicated that KNTC1 knockdown weakened the invasion and migration abilities of SiHa and C-33A cells and decreased the expression of MMP-2 and MMP-9. In-vivo experiments suggested that the inhibition of KNTC1 reduced tumor growth. Taken together, our study showed that KNTC1 plays an important role in cervical cancer. Further, we verified the promotional effect of KNTC1 on cervical cancer through in-vivo and in-vitro experiments and speculated that KNTC1 might mediate tumor invasion via MMP9 and MMP2.", "claims": [{"id": "claim-method", "statement": "Wilcoxon rank sum test results showed that the expression level of KNTC1 in tumor group was higher than normal, and the median difference between the two groups was 2.694 (2.081–3.311), which was statistically significant (P = 0.003) (figure 1f).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35389773", "locator": "sections[14] \"Bioinformatics results\" paragraph 2", "quote": "Wilcoxon rank sum test results showed that the expression level of KNTC1 in tumor group was higher than normal, and the median difference between the two groups was 2.694 (2.081–3.311), which was statistically significant (P = 0.003) (figure 1f)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Cervical cancer GSE63514 series from the Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/) database was obtained using the GEOquery package to further organize and standardize the data [27]. Subsequently, the limma package was used for differential gene analysis [28], and the clusterProfiler package was used for gene ontology (GO)/Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis [29].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35389773", "locator": "sections[3] \"Bioinformatics analysis\" paragraph 2", "quote": "Cervical cancer GSE63514 series from the Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/) database was obtained using the GEOquery package to further organize and standardize the data [27]. Subsequently, the limma package was used for differential gene analysis [28], and the clusterProfiler package was used for gene ontology (GO)/Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis [29]."}], "state": "complete"}, {"id": "claim-target-result", "statement": "KNTC1 expression was significantly higher in cervical cancer tumor tissue than in normal tissue, with a median difference of 2.694.", "kind": "quantitative", "target": {"value": 0.003, "unit": "p-value", "tolerance": null, "source": "Wilcoxon rank sum test results showed that the expression level of KNTC1 in tumor group was higher than normal, and the median difference between the two groups was 2.694 (2.081–3.311), which was statistically significant (P = 0.003) (figure 1f)."}, "evidence": [{"asset": "source:pmid:35389773", "locator": "sections[14] \"Bioinformatics results\" paragraph 2", "quote": "Wilcoxon rank sum test results showed that the expression level of KNTC1 in tumor group was higher than normal, and the median difference between the two groups was 2.694 (2.081–3.311), which was statistically significant (P = 0.003) (figure 1f)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "KNTC1 expression was significantly higher in cervical cancer tumor tissue than in normal tissue, with a median difference of 2.694.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35389773"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Wound healing", "constructed to analyze tumor growth in vivo. CCK8 and colony formation assay results demonstra"], "order_basis": "paper_text_order:750", "id": "assay:1"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["x metallopeptidase 9 (MMP9) were measured by western blotting. Nude mouse models of subcutaneous xenograft", "33A cells decreased when KNTC1 was silenced. Western blot and Transwell assays indicated that KNTC1 kn", "2 weeks of puromycin selection, qRT-PCR and western blotting were used to verify knockdown efficiency. An"], "order_basis": "paper_text_order:997", "id": "assay:2"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["mor were constructed to analyze tumor growth in vivo. CCK8 and colony formation assay results dem", "MP9) were measured by western blotting. Nude mouse models of subcutaneous xenograft tumor were", "ent Center of Xi’an Jiaotong University. The mice were randomly divided into two groups and shKNTC1", "width × width/2. Thirty days later, the nude mice were euthanized by cervical dislocation after bei"], "order_basis": "paper_text_order:1020", "id": "assay:3"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ot and Transwell assays indicated that KNTC1 knockdown weakened the invasion and migration abilitie", "Materials and methods Cell culture and transfection Cervical cancer cell lines SiHa and C-33A, a", "and right armpits of each group. SiHa cells transfected with the lentivirus were injected into the r", "PCR and western blotting were used to verify knockdown efficiency. Animals and treatment Six female"], "order_basis": "paper_text_order:1310", "id": "assay:4"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["Procell (Wuhan, China). SiHa and C-33A were cultured in minimum essential medium containing 10% feta", "fetal bovine serum, and HcerEpic cells were cultured in complete medium for human cervical epithelia"], "order_basis": "paper_text_order:2016", "id": "assay:5"}, {"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Wilcoxon rank sum test results showed that the expression level of KNTC1 in tumor group was higher than normal, and the median difference between the two groups was 2.694 (2.081–3.311), which was statistically significant (P = 0.003) (figure 1f)."], "order_basis": "consumes_earlier_output", "id": "assay:6"}]}