Integrative analysis of key microRNA-mRNA complexes and pathways in aortic aneurysm.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35433971", "kind": "science-record", "revision": 5, "content_hash": "sha256:0bff5648b9270d9a6eec6a52de06094a6f93338a300a673ba95a0d89fe3b6a85", "created_at": "2026-11-01T18:14:17Z", "updated_at": "2026-11-01T22:48:52Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Integrative analysis of key microRNA-mRNA complexes and pathways in aortic aneurysm.", "doi": "10.21037/atm-22-514", "pmid": "35433971", "authors": ["Yufei Fu", "Yingying Mao", "Ruoshi Chen", "Anfeng Yu", "Yifei Yin", "Xi Wang", "Liang Ma", "Xin Chen"], "journal": "Annals of translational medicine", "year": 2022, "abstract": "Background: This study investigated the therapeutic targets of aortic aneurysm (AA) and provided insights into the pathogenesis and molecular mechanisms of AA.\nMethods: The messenger RNA (mRNA) datasets, GSE9106 (blood samples) and GSE7084 (tissue samples), and the microRNA (miRNA) datasets, GSE92427 (blood samples) and GSE110527 (tissue samples), were obtained from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) and differentially expressed miRNAs (DE-miRNAs) were analyzed by limma. Based on the co-DEGs and co-DE-miRNAs between the AA blood and tissue datasets, the miRNA-mRNA regulatory pairs were predicted. Functional enrichment analyses and gene set enrichment analysis (GSEA) were performed and the protein-protein interaction (PPI) network was generated to further analyze the related genes and their functions. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and tyramide signal amplification (TSA)-in situ hybridization (ISH) assays were performed to detect the expression of co-DE-miRNAs in AA clinical tissue samples and normal aorta samples.\nResults: There were 19 upregulated and 5 downregulated co-differential mRNAs. MiR-4306 was the upregulated co-differential miRNA, and miR-3198 was the downregulated co-differential miRNA by blood-tissue co-analysis. Based on the co-DEGs and co-DE-miRNAs, 4 miRNA-mRNA regulatory pairs were predicted. PPI networks were constructed of co-DEGs with 6 relationship pairs. RT-qPCR and TSA-ISH assays showed the upregulation of miR-4306 and the downregulation of miR-3198 in AA tissue samples.\nConclusions: This study provided evidence regarding the differential regulatory miRNA-mRNA networks in AA blood and tissue samples and identified key genes and signaling pathways related to AA, which provided insights into potential targets and mechanisms of AA pathogenesis and progression.", "claims": [{"id": "claim-method", "statement": "The expression matrices of mRNA datasets GSE9106 and GSE7084 were analyzed by limma (9) to identify the differentially expressed genes (DEGs) between the AA samples and the control samples.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35433971", "locator": "sections[4] \"Analyses of differentially expressed mRNAs and miRNAs\" paragraph 2", "quote": "The expression matrices of mRNA datasets GSE9106 and GSE7084 were analyzed by limma (9) to identify the differentially expressed genes (DEGs) between the AA samples and the control samples."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data Sharing Statement: Available at https://atm.amegroups.com/article/view/10.21037/atm-22-514/dss", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35433971", "locator": "sections[25] \"(untitled)\" paragraph 1", "quote": "Data Sharing Statement: Available at https://atm.amegroups.com/article/view/10.21037/atm-22-514/dss"}], "state": "complete"}, {"id": "claim-target-result", "statement": "There were 19 upregulated and 5 downregulated co-differential mRNAs.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35433971", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "There were 19 upregulated and 5 downregulated co-differential mRNAs."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "There were 19 upregulated and 5 downregulated co-differential mRNAs.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35433971"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE110527"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE110527"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:35433971", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.21037/atm-22-514", "sha256": 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