[Bioinformatics screening and analysis of key differentially expressed genes characteristics in nonalcoholic fatty liver disease].

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35462486", "kind": "science-record", "revision": 4, "content_hash": "sha256:c728b7763a1a55b52a48fecff1c3669fde2f4def88537c4b434a383b79544492", "created_at": "2026-11-01T18:14:18Z", "updated_at": "2026-11-01T22:06:22Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "[Bioinformatics screening and analysis of key differentially expressed genes characteristics in nonalcoholic fatty liver disease].", "doi": "10.3760/cma.j.cn501113-20210525-00251", "pmid": "35462486", "authors": ["J X Ding", "W B Huang", "X X Jiang", "L D Zhang", "H Fang", "J Jin"], "journal": "Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology", "year": 2022, "abstract": "Objective: To screen and analyze the key differentially expressed genes characteristics in nonalcoholic fatty liver disease (NAFLD) with bioinformatics method. Methods: NAFLD-related expression matrix GSE89632 was downloaded from the GEO database. Limma package was used to screen differentially expressed genes (DEGs) in healthy, steatosis (SS), and nonalcoholic steatohepatitis (NASH) samples. WGCNA was used to analyze the output gene module. The intersection of module genes and differential genes was used to determine the differential genes characteristic, and then GO function and KEGG signaling pathway enrichment analysis were performed. The protein-protein interaction network (PPI) was constructed using the online website STRING and Cytoscape software, and the key (Hub) genes were screened. Finally, R software was used to analyze the receiver operating characteristic curve (ROC) of the Hub gene. Results: 92 differentially expressed genes characteristic were obtained through screening, which were mainly enriched in inflammatory response-related functions of \"lipopolysaccharide response and molecular response of bacterial origin\", as well as cancer signaling pathways of \"proteoglycan in cancer\" and \"T-cell leukemia virus infection-related\". 10 hub genes (FOS, CXCL8, SERPINE1, CYR61, THBS1, FOSL1, CCL2, MYC, SOCS3 and ATF3) had good diagnostic value. Conclusion: The differentially expressed hub genes among the 10 NAFLD disease-related characteristics obtained with bioinformatics analysis may become a diagnostic and prognostic marker and potential therapeutic target for NAFLD. However, further basic and clinical studies are needed to validate.", "claims": [{"id": "claim-method", "statement": "使用Limma包筛选健康样本、脂肪变性（SS）样本和非酒精性脂肪性肝炎（NASH）样本差异表达基因（DEGs），WGCNA分析输出模块基因", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35462486", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "使用Limma包筛选健康样本、脂肪变性（SS）样本和非酒精性脂肪性肝炎（NASH）样本差异表达基因（DEGs），WGCNA分析输出模块基因"}], "state": "complete"}, {"id": "claim-data-access", "statement": "GEO数据库中下载NAFLD相关的表达矩阵GSE89632", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35462486", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "GEO数据库中下载NAFLD相关的表达矩阵GSE89632"}], "state": "complete"}, {"id": "claim-target-result", "statement": "经筛选共获得92个特征差异表达基因，主要富集于“脂多糖反应和细菌起源分子反应”的炎症反应相关功能，以及“癌症中蛋白聚糖”和“T细胞白血病病毒感染相关”癌症信号通路。10个Hub基因（FOS，CXCL8，SERPINE1，CYR61，THBS1，FOSL1，CCL2，MYC，SOCS3和ATF3）具有较好的诊断价值。", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35462486", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "经筛选共获得92个特征差异表达基因，主要富集于“脂多糖反应和细菌起源分子反应”的炎症反应相关功能，以及“癌症中蛋白聚糖”和“T细胞白血病病毒感染相关”癌症信号通路。10个Hub基因（FOS，CXCL8，SERPINE1，CYR61，THBS1，FOSL1，CCL2，MYC，SOCS3和ATF3）具有较好的诊断价值。"}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "经筛选共获得92个特征差异表达基因，主要富集于“脂多糖反应和细菌起源分子反应”的炎症反应相关功能，以及“癌症中蛋白聚糖”和“T细胞白血病病毒感染相关”癌症信号通路。10个Hub基因（FOS，CXCL8，SERPINE1，CYR61，THBS1，FOSL1，CCL2，MYC，SOCS3和ATF3）具有较好的诊断价值。", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35462486"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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