Integrated Multichip Analysis and WGCNA Identify Potential Diagnostic Markers in the Pathogenesis of ST-Elevation Myocardial Infarction.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35475279", "kind": "science-record", "revision": 5, "content_hash": "sha256:991275e99104ceb59df916b980fda43c7336fcdeb47a34fd67d233e8f36a9590", "created_at": "2026-11-01T18:14:20Z", "updated_at": "2026-11-01T22:48:56Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Integrated Multichip Analysis and WGCNA Identify Potential Diagnostic Markers in the Pathogenesis of ST-Elevation Myocardial Infarction.", "doi": "10.1155/2022/7343412", "pmid": "35475279", "authors": ["Yingliang Liang", "Wandang Wang", "Qiufang Huang", "Hui Chen"], "journal": "Contrast media & molecular imaging", "year": 2022, "abstract": "Background: ST-elevation myocardial infarction (STEMI) is a myocardial infarction (MI) with ST-segment exaltation of electrocardiogram (ECG) caused by vascular occlusion of the epicardium. However, the diagnostic markers of STEMI remain little.\nMethods: STEMI raw microarray data are acquired from the Gene Expression Omnibus (GEO) database. Based on GSE60993 and GSE61144, differentially expressed genes (DEGs) are verified via R software, and key modules associated with pathological state of STEMI are verified by weighted correlation network analysis (WGCNA). Take the intersection gene of key module and DEGs to perform the pathway enrichment analyses by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Construct the protein-protein interaction (PPI) network by Cytoscape. Then, select and identify the diagnostic biomarkers of STEMI by least absolute shrinkage and selection operator (LASSO) logistic regression and support vector machine-recursive feature elimination (SVM-RFE) algorithms. Finally, assess the infiltration of immune cells of STEMI by CIBERSORT and analyze the correlation between diagnostic markers and infiltrating immune cells.\nResults: We get 710 DEGs in the STEMI group and 376 genes associated with STEMI in blue module. 92 intersection genes were concentrated in 30 GO terms and 2 KEGG pathways. 28 hub genes involved in the development of STEMI. Moreover, upregulated ALOX5AP (AUC = 1.00) and BST1 (AUC = 1.00) are confirmed as diagnostic markers of STEMI. CD8+T cells, regulatory T (Treg) cells, resting natural killer (NK) cells, M0 macrophages, resting mast cells, and neutrophils are related to the procession of STEMI. Moreover, ALOX5AP and BST1 are positively related to resting NK cells, M0 macrophages, and neutrophils, while ALOX5AP and BST1 are negatively related to CD8+ T cells, Treg cells, and resting mast cells.\nConclusion: ALOX5AP and BST1 may be the diagnostic markers of STEMI. Immune cell infiltration plays a key role in the development of STEMI.", "claims": [{"id": "claim-method", "statement": "Identify 7 genes from hub genes as diagnostic markers for STEMI by the LASSO logistic regression algorithm (Figure 6(a)); 2 genes as diagnostic markers of STEMI are obtained from hub genes by the SVM-RFE algorithm (Figure 6(b)).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35475279", "locator": "sections[14] \"3.4. Screen and Verify the Diagnostic Markers\" paragraph 2", "quote": "Identify 7 genes from hub genes as diagnostic markers for STEMI by the LASSO logistic regression algorithm (Figure 6(a)); 2 genes as diagnostic markers of STEMI are obtained from hub genes by the SVM-RFE algorithm (Figure 6(b))."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data used to support the findings of this study are available from the corresponding author upon request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35475279", "locator": "sections[18] \"Data Availability\" paragraph 2", "quote": "The data used to support the findings of this study are available from the corresponding author upon request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Upregulation of ALOX5AP (AUC = 1.00) and BST1 (AUC = 1.00) have a high diagnostic value for STEMI.", "kind": "quantitative", "target": {"value": 1.0, "unit": "auc", "tolerance": null, "source": "Upregulation of ALOX5AP (AUC = 1.00) and BST1 (AUC = 1.00) have a high diagnostic value for STEMI (Figures 7(a) and 7(b))."}, "evidence": [{"asset": "source:pmid:35475279", "locator": "sections[14] \"3.4. Screen and Verify the Diagnostic Markers\" paragraph 2", "quote": "Upregulation of ALOX5AP (AUC = 1.00) and BST1 (AUC = 1.00) have a high diagnostic value for STEMI (Figures 7(a) and 7(b))."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Upregulation of ALOX5AP (AUC = 1.00) and BST1 (AUC = 1.00) have a high diagnostic value for STEMI.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35475279"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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