LANCL1 as the Key Immune Marker in Neuropathic Pain.

{"sci_version": "1.0", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35510269", "kind": "science-record", "revision": 3, "content_hash": "sha256:d91c7a8de3be729f2a4cdd4bebabd97bfd350559e120f65fb3d4a081617c1799", "created_at": "2026-11-01T18:14:23Z", "updated_at": "2026-11-01T21:27:47Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "LANCL1 as the Key Immune Marker in Neuropathic Pain.", "doi": "10.1155/2022/9762244", "pmid": "35510269", "authors": ["Yu Shi", "XueFei Zhang", "Qian Fang", "Hongrui Zhan", "Xianglong Wang", "Xiyan Huang", "Tao Fan", "Wei Liu", "Wen Wu"], "journal": "Neural plasticity", "year": 2022, "abstract": "Objective: This study is to explore key immune markers and changes of immune microenvironment in neuropathic pain (NeuP).\nMethod: The data sets of GSE145199 and GSE145226 in Gene Expression Omnibus (GEO) database was used to analyze, and the key immune markers were verified by GSE70006 and GSE91396, and the infiltration degree of immune cells in different samples were analyzed by CIBERSORT analysis package.\nResults: In this study, we found a key immune marker, namely, LANCL1. Regulatory axis closely related to LANCL1 has also been found, namely, miR-6325/LANCL1 axis. In the immune infiltration analysis, we also found that the LANCL1 is positively correlated with T cells CD4 naïve (r = 0.880, p < 0.05).\nConclusion: In this study, we found that LANCL1 may be a protective factor for NeuP, and the miR-6325/LANCL1 axis may be involved in the occurrence and development of NeuP. Cascade reactions including mast cells, macrophages, and T cells may be an important reason for the aggravation of nerve damage.", "claims": [{"id": "claim-method", "statement": "In this step, we used the reshape2 [27], dplyr (https://dplyr.tidyverse.org/), and tidyr (https://tidyr.tidyverse.org) analysis packages for the correlation analysis between mRNA and miRNA.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35510269", "locator": "sections[9] \"2.7. Correlation Analysis of mRNAs (Key Immune Markers) and miRNAs\" paragraph 2", "quote": "In this step, we used the reshape2 [27], dplyr (https://dplyr.tidyverse.org/), and tidyr (https://tidyr.tidyverse.org) analysis packages for the correlation analysis between mRNA and miRNA."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The sequencing data used to support the findings of this study have been deposited in the GEO repository (GSE145199, GSE145226, GSE70006, and GSE91396).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35510269", "locator": "sections[25] \"Data Availability\" paragraph 2", "quote": "The sequencing data used to support the findings of this study have been deposited in the GEO repository (GSE145199, GSE145226, GSE70006, and GSE91396)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "LANCL1 was positively correlated with T cells CD4 naïve (r = 0.880, p < 0.05) (Figure 7(b)).", "kind": "quantitative", "target": {"value": 0.88, "unit": "correlation coefficient", "tolerance": null, "source": "LANCL1 was positively correlated with T cells CD4 naïve (r = 0.880, p < 0.05) (Figure 7(b))."}, "evidence": [{"asset": "source:pmid:35510269", "locator": "sections[20] \"3.6. Correlation Analysis Results between Genes and Immune Infiltrating Cells\" paragraph 2", "quote": "LANCL1 was positively correlated with T cells CD4 naïve (r = 0.880, p < 0.05) (Figure 7(b))."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "LANCL1 was positively correlated with T cells CD4 naïve (r = 0.880, p < 0.05) (Figure 7(b)).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35510269"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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