GEO Database Screening Combined with In Vitro Experiments to Study the Mechanism of hsa_circ_0003570 in Infantile Hemangiomas.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35529258", "kind": "science-record", "revision": 4, "content_hash": "sha256:f98267c7d75fff175f6e542631e6cd9de9d229b43aa3f29976a8b01e9f124658", "created_at": "2026-11-01T18:14:25Z", "updated_at": "2026-11-01T22:06:29Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "GEO Database Screening Combined with In Vitro Experiments to Study the Mechanism of hsa_circ_0003570 in Infantile Hemangiomas.", "doi": "10.1155/2022/5643742", "pmid": "35529258", "authors": ["Yu Tian", "Shihao Zhuang", "Jiantao Zhang", "Tonghui You", "Zihang Xu", "Wanli Yang", "Bin Hao", "Weifeng Wang", "Tao Yang"], "journal": "Computational and mathematical methods in medicine", "year": 2022, "abstract": "Objective: In this study, we screened out a type of differentially expressed circular RNA in infantile hemangioma (IH) cells and analyzed the mechanism in the malignant biological behavior of IH.\nMethods: Based on the GSE98795, GSE100682, and GSE43742 datasets, differential expression analysis of circRNAs, microRNAs, and mRNAs was performed. The relative expression level of RNA was detected by quantitative real-time polymerase chain reaction (qRT-PCR). MTT assay, Transwell, flow cytometry analysis, and western blot were used to study the effects of hsa_circ_0003570, hsa-miR-138-5p, and RGS5 on the proliferation and apoptosis of hemangioma endothelial cells (HEMECs).\nResults: The hsa_circ_0003570 and RGS5 mRNA were upregulated in HEMECs, but hsa-miR-138-5p was downregulated. Silencing of hsa_circ_0003570 inhibited the proliferation of HEMECs and promoted the apoptosis of HEMECs. The malignant biological behaviors of hsa_circ_0003570 on the proliferation and apoptosis of HEMECs were reversed by hsa-miR-138-5p. Hsa_circ_0003570 acted as the ceRNA of hsa-miR-138-5p and upregulated the expression of RGS5. Silencing of RGS5 inhibited the proliferation, migration, and invasion of HEMECs and promoted apoptosis.\nConclusion: Hsa_circ_0003570 promotes IH cell proliferation and inhibits IH cell apoptosis through hsa-miR-138-5p/RGS5 axis.", "claims": [{"id": "claim-method", "statement": "The results of GEO2R analysis showed that a total of 234 circRNAs were upregulated and 374 circRNAs were downregulated (FC (abs) cut-off value 1.0) in the infantile hemangioma tumor tissue.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35529258", "locator": "sections[25] \"Supplementary Materials\" paragraph 2", "quote": "The results of GEO2R analysis showed that a total of 234 circRNAs were upregulated and 374 circRNAs were downregulated (FC (abs) cut-off value 1.0) in the infantile hemangioma tumor tissue."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data used to support the findings of this study are available from the corresponding author upon request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35529258", "locator": "sections[22] \"Data Availability\" paragraph 2", "quote": "The data used to support the findings of this study are available from the corresponding author upon request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Hsa_circ_0003570 promotes IH cell proliferation and inhibits IH cell apoptosis through hsa-miR-138-5p/RGS5 axis.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35529258", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "Hsa_circ_0003570 promotes IH cell proliferation and inhibits IH cell apoptosis through hsa-miR-138-5p/RGS5 axis."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "Hsa_circ_0003570 promotes IH cell proliferation and inhibits IH cell apoptosis through hsa-miR-138-5p/RGS5 axis.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35529258"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Re", "the proliferation of HEMECs and promoted the apoptosis of HEMECs. The malignant biological behavior", "of hsa_circ_0003570 on the proliferation and apoptosis of HEMECs were reversed by hsa-miR-138-5p. H", "gration, and invasion of HEMECs and promoted apoptosis. Conclusion: Hsa_circ_0003570 promotes IH ce"], "order_basis": "paper_text_order:539", "id": "assay:1"}, {"role": "data_generation", "definition_id": "flow_cytometry", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["in reaction (qRT-PCR). MTT assay, Transwell, flow cytometry analysis, and western blot were used to stud"], "order_basis": "paper_text_order:561", "id": "assay:2"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["say, Transwell, flow cytometry analysis, and western blot were used to study the effects of hsa_circ_0"], "order_basis": "paper_text_order:590", "id": "assay:3"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.77, "evidence": ["endothelial cells (HDMVECs) and HEMECs were cultured in DMEM (Invitrogen, Carlsbad, CA, USA) with 10"], "order_basis": "paper_text_order:1545", "id": "assay:4"}, {"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["The results of GEO2R analysis showed that a total of 234 circRNAs were upregulated and 374 circRNAs were downregulated (FC (abs) cut-off value 1.0) in the infantile hemangioma tumor tissue."], "order_basis": "consumes_earlier_output", "id": "assay:5"}]}