Exploration of the Potential Mechanism of the Common Differentially Expressed Genes in Psoriasis and Atopic Dermatitis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35586812", "kind": "science-record", "revision": 5, "content_hash": "sha256:623d600f20a24ee2e5de2401e13c0a80802a69acfce08e78680fabae82e8494e", "created_at": "2026-11-01T18:14:29Z", "updated_at": "2026-11-01T22:49:05Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Exploration of the Potential Mechanism of the Common Differentially Expressed Genes in Psoriasis and Atopic Dermatitis.", "doi": "10.1155/2022/1177299", "pmid": "35586812", "authors": ["Zhiyu Zhou", "Li Meng", "Yawen Cai", "Wannian Yan", "Yun Bai", "Jia Chen"], "journal": "BioMed research international", "year": 2022, "abstract": "Backgrounds: Psoriasis and atopic dermatitis are two common chronic inflammatory skin diseases that enormously deteriorate the psycho-physical and socio-economic condition of the patients. Although differential immune responses have been found to operate in the pathomechanisms of atopic dermatitis and psoriasis, the epidermal keratinocytes are the major targets in both diseases, and sometimes, they show similar clinical presentations. The skin barrier, itching, and inflammation are current and future treatment targets for both of them, but the relevant shared mechanisms of the two diseases are far from understood.\nMethods: The differential analyses of GSE14905 (psoriasis) and GSE32924 (atopic dermatitis) deposited in GEO database were conducted and obtained their differential expressed genes. Moreover, PPI, functional modules, GO, and KEGG enrichment analyses were used for the further analysis. The mouse models of psoriasis and atopic dermatitis were established, and then, RT-qPCR and Western blotting assay were performed to check the abundant changes of hub genes.\nResults: There are 732 differentially expressed genes in psoriasis versus nonlesional skin samples. Besides, 611 differentially expressed genes were identified in atopic dermatitis versus nonlesional skin data sets. Based on these differentially expressed genes, we predicted their joint and individual protein-protein interaction networks and functional modules in both psoriasis and atopic dermatitis. Through the PPI network of genes, we calculated the hub nodes and do the GO and KEGG enrichment analysis of overlapped genes of psoriasis and atopic dermatitis, which suggested there were some terms like \"positive regulation of interleukin-12 production,\" \"centromeric region,\" and \"TNF signaling pathway.\"\nConclusion: We constructed the predicted PPI networks and functional modules related to psoriasis and atopic dermatitis and distinguished the key candidate target genes CXCL8, STAT1, and MMP9 in the diagnosis and therapy of similar pathogenesis.", "claims": [{"id": "claim-method", "statement": "R package limma [28] was used to perform the different expression analysis of genes between the psoriasis/atopic-dermatitis samples and the healthy samples using the expression matrix.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35586812", "locator": "sections[4] \"2.2. Data Preprocessing\" paragraph 2", "quote": "R package limma [28] was used to perform the different expression analysis of genes between the psoriasis/atopic-dermatitis samples and the healthy samples using the expression matrix."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data used to support the findings of this study are available from the corresponding author upon request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35586812", "locator": "sections[19] \"Data Availability\" paragraph 2", "quote": "The data used to support the findings of this study are available from the corresponding author upon request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Differential-expression analysis identified 732 differentially expressed genes in psoriasis versus nonlesional skin samples and 611 differentially expressed genes in atopic dermatitis versus nonlesional skin samples.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35586812", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "There are 732 differentially expressed genes in psoriasis versus nonlesional skin samples."}, {"asset": "source:pmid:35586812", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "Besides, 611 differentially expressed genes were identified in atopic dermatitis versus nonlesional skin data sets."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Differential-expression analysis identified 732 differentially expressed genes in psoriasis versus nonlesional skin samples and 611 differentially expressed genes in atopic dermatitis versus nonlesional skin samples.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35586812"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE14905"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE14905"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:35586812", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1155/2022/1177299", "sha256": 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The mouse models of psoriasis and atopic dermatitis we", "ly on the back of mice daily for 21days. The mice were sacrificed on the 22nd day of modeling, and the skin le", "ly on the back of mice daily for 21days. The mice were sacrificed on the 22nd day of modeling, and"], "order_basis": "paper_text_order:987", "id": "assay:1"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["topic dermatitis were established, and then, RT-qPCR and Western blotting assay were performed to", "ic dermatitis were established, and then, RT-qPCR and Western blotting assay were performed to"], "order_basis": "paper_text_order:1063", "id": "assay:2"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["itis were established, and then, RT-qPCR and Western blotting assay were performed to check the abundant c"], "order_basis": "paper_text_order:1075", "id": "assay:3"}, {"role": "analysis", "definition_id": "differential_expression", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["normalise_counts", "differential_expression_fit", "fdr_bh"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["R package limma [28] was used to perform the different expression analysis of genes between the psoriasis/atopic-dermatitis samples and the healthy samples using the expression matrix."], "order_basis": "consumes_earlier_output", "id": "assay:4"}]}