MicroRNA-744-5p suppresses tumorigenesis and metastasis of osteosarcoma through the p38 mitogen-activated protein kinases pathway by targeting transforming growth factor-beta 1.

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Accumulating evidence has revealed that microRNAs (miRNAs) play a crucial role in the progression of OS. In this study, we found that miR-744-5p was the least expressed miRNA in patients with OS by analyzing GSE65071 from the GENE EXPRESSION OMNIBUS (GEO) database. Through real-time quantitative PCR (qRT-PCR), western blotting, colony formation assay, 5-Ethynyl-2-Deoxyuridine (EdU) incorporation assay, transwell migration, and invasion assays, we demonstrated its ability to inhibit the proliferation, migration, and invasion of OS cells in vitro. According to the luciferase reporter assay, transforming growth factor-β1 (TGFB1) was negatively regulated by miR-744-5p and reversed the effects of miR-744-5p on OS. Subcutaneous tumor-forming animal models and tail vein injection lung metastatic models were used in animal experiments, and it was found that miR-744-5p negatively regulated tumor growth and metastasis in vivo. Furthermore, rescue assays verified that miR-744-5p regulates TGFB1 expression in OS. Further experiments revealed that the p38 MAPK signaling pathway is involved in the miR-744-5p/TGFB1 axis. Generally, this study suggests that miR-744-5p is a negative regulator of TGFB1 and suppresses OS progression and metastasis via the p38 MAPK signaling pathway.", "claims": [{"id": "claim-method", "statement": "The R package limma was used to detect differences in miRNA expression levels between normal and OS samples. The filter criterion was log [fold change (FC)] > 1 and adjusted P-value < 0.05 [35].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35593122", "locator": "sections[5] \"Obtainment and analysis of original data\" paragraph 2", "quote": "The R package limma was used to detect differences in miRNA expression levels between normal and OS samples. 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Mice were sacrificed at the end of the experiment. Statistical an", "with 5% CO2 at 37°C. Animal experiments Nude mice were purchased from the Animal Core Facility of S", "umors were separated and imaged on day 28th. Mice were sacrificed at the end of the experiment. Sta"], "order_basis": "paper_text_order:1142", "id": "assay:3"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["llection (ATCC, Manassas, US). OS cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; In", "in (PS; Gibco, CA). 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