Characterization of the Prognostic Values of CXCL Family in Epstein-Barr Virus Associated Gastric Cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35693706", "kind": "science-record", "revision": 5, "content_hash": "sha256:13c9b1bfae680e8a358f784089de4dce1c84f1513f28d1f77f3a0c80d254deea", "created_at": "2026-11-01T18:14:39Z", "updated_at": "2026-11-01T22:49:17Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Characterization of the Prognostic Values of CXCL Family in Epstein-Barr Virus Associated Gastric Cancer.", "doi": "10.1155/2022/2218140", "pmid": "35693706", "authors": ["Li Mu", "Shun Hu", "Guoping Li", "Ping Wu", "Caihong Ren", "Taiyu Lin", "Sheng Zhang"], "journal": "Oxidative medicine and cellular longevity", "year": 2022, "abstract": "Background: CXCL family is a class of secreted growth factors signaling through G-protein-coupled receptors, and abnormal expression is associated with the growth and progression of many tumors. However, their prognostic value has been poorly studied in Epstein-Barr virus- (EBV-) associated gastric cancer (EBVaGC). Therefore, it is of great significance to explore the prognostic value of the CXCL family in EBVaGC.\nMethods: CXCL family mRNA expression was analyzed in STAD data from The Cancer Genome Atlas (TCGA). Kaplan-Meier Plotter was used to assess the prognostic value of the CXCL family. Transcription factors (TFs) and miRNAs associated with the CXCL family were identified by TFCheckpoint, miRWalk, and ViRBase databases. The prognostic model was evaluated using the EBVaGC patient cohort GSE51575.\nResults: The mRNA expression of CXCL1/3/5/6/8/9/10/11/16 was significantly upregulated, while the expression of CXCL12/14 was downregulated in EBVaGC compared with normal tissues from TCGA-STAD. The mRNA expressions of CXCL9, CXCL10, CXCL11, and CXCL17 in EBVaGCs were higher than those in EBVnGCs, but the mRNA expressions of CXCL6, CXCL12, and CXCL17 were lower than those in EBVnGCs. The mRNA expression levels of CXCL9, CXCL10, and CXCL11 in EBVaGCs were higher than those in EBVnGCs regardless of the tumor stage. High mRNA expression of CXCL8 was associated with better OS in patients with EBVaGC, while high expression of CXCL9 was associated with better OS in patients with EBVnGC. We obtained 10 candidate potential transcription factors (TFs) associated with CXCLs: OTOP3, NKX6-2, NKX2-2, FEV, SMYD1, TRIMSO, TBX10, CDX1, SLC26A3, and ARC. 576 miRNA-mRNA interactions were obtained. Among them, 65 miRNAs were predicted to be correlated with CXCL6, CXCL9, CXCL10, and CXCL11. Similar to the results of TCGA-STAD, the GSE51575 dataset also showed that the mRNA expression levels of CXCL1/3/9/10/11/16 were markedly enhanced in EBVaGC tissues compared with corresponding normal gastric mucosa tissues, while the mRNA expression levels of CXCL12/14 were significantly reduced. The mRNA expression levels of CXCL3/9/10/11/13/17 were increased in EBVaGC compared with EBVnGC tissues.\nConclusions: The expression differences of CXCL family members are closely associated with the progression of EBVaGC. Expression of CXCL9/10/11/17 mRNA may be a promising prognostic indicator for EBVaGC patients.", "claims": [{"id": "claim-method", "statement": "Taking the median value of each CXCL in the sample as the threshold value, the CXCL was divided into two groups of high expression or low expression, and the P value of log-rank was calculated.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35693706", "locator": "sections[5] \"2.3. Prognostic Value of CXCL Family Members between EBVaGC and EBVnGC\" paragraph 2", "quote": "Taking the median value of each CXCL in the sample as the threshold value, the CXCL was divided into two groups of high expression or low expression, and the P value of log-rank was calculated."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The labeled dataset used to support the findings of this study is available from the corresponding author upon request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35693706", "locator": "sections[18] \"Data Availability\" paragraph 2", "quote": "The labeled dataset used to support the findings of this study is available from the corresponding author upon request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "High CXCL8 mRNA expression was significantly associated with better OS in patients with EBVaGC (P = 0.027), while high CXCL9 mRNA expression was significantly associated with better OS in patients with EBVnGC (P = 0.049).", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35693706", "locator": "sections[13] \"3.4. Prognostic Value of CXCL Family Members in GCs\" paragraph 2", "quote": "high CXCL8 mRNA expression was significantly associated with better OS in patients with EBVaGC (P = 0.027), while high CXCL9 mRNA expression was significantly associated with better OS in patients with EBVnGC (P = 0.049)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "High CXCL8 mRNA expression was significantly associated with better OS in patients with EBVaGC (P = 0.027), while high CXCL9 mRNA expression was significantly associated with better OS in patients with EBVnGC (P = 0.049).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35693706"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE51575"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE51575"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:35693706", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1155/2022/2218140", "sha256": 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