LINC00461 Regulates the Recurrence of Large B Cell Lymphoma through the miR-411-5p/BNIP3 Pathway.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35783530", "kind": "science-record", "revision": 5, "content_hash": "sha256:fe548c15c384c51faef7885015cb02f4367a4c26aeea0aba6629467ce71f20c4", "created_at": "2026-11-01T18:14:49Z", "updated_at": "2026-11-01T22:49:27Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "LINC00461 Regulates the Recurrence of Large B Cell Lymphoma through the miR-411-5p/BNIP3 Pathway.", "doi": "10.1155/2022/9100056", "pmid": "35783530", "authors": ["Shu-Wen Sun", "Yan Chen", "Hui-Juan Liao", "Wei Zhang", "Wen-Ming Xu", "Guo-Qian He"], "journal": "Evidence-based complementary and alternative medicine : eCAM", "year": 2022, "abstract": "Objective: To analyze the mechanism of LINC00461 regulating the recurrence of diffuse large B cell lymphoma (DLBCL) through microRNA (miR)-411-5p/BCL2 interacting protein 3 (BNIP3) pathway.\nMethods: DLBCL samples in TCGA and GSE12453 were used for differential analysis to find long noncoding RNA (lncRNA) related to DLBCL recurrence. The 4 DLBCL data with the highest and lowest expression levels of LINC00461 in the TCGA database were selected for GSEA enrichment analysis. The targeting relationships of miR-411-5p with LINC00461 and BNIP3 were verified by the dual luciferase report. Blood samples from DLBCL patients were used to analyze the correlation between miR-411-5p and LINC00461 or BNIP3. LINC00461, miR-411-5p, or BNIP3 was overexpressed or silenced by transfection, and a tumor-bearing nude mice model was constructed to detect their effects on proliferation and apoptosis.\nResults: The level of LINC00461 in DLBCL was significantly higher than that in normal cases, and the level in recurrence DLBCL was significantly higher than that in nonrecurrence. The enrichment analysis results showed that the function of LINC00461 was closely related to apoptosis. The results shown that miR-411-5p bound to LINC00461 and BNIP3 and was negatively correlated with LINC00461 and BNIP3 mRNA in blood of DLBCL patients. Suppressing the level of LINC00461 inhibited cell proliferation and induced apoptosis. The inhibition of LINC00461 or overexpression of miR-411-5p reduced the expression of BNIP3 protein, thereby inducing apoptosis at the in vivo and in vitro levels.\nConclusion: LINC00461 may induce miR-411-5p to \"sponge,\" thereby increasing the expression of BNIP3 protein, and exerting the function of inhibiting apoptosis and promoting DLBCL recurrence.", "claims": [{"id": "claim-method", "statement": "Student's t-test was used for the comparison between two groups. For the comparison among at least three groups, one-way analysis of variance (ANOVA) was applied. The Pearson test was used to analyze the correlation between miR-411-5p and LINC00461 and BNIP3.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35783530", "locator": "sections[14] \"2.12. Statistical Analysis\" paragraph 2", "quote": "Student's t-test was used for the comparison between two groups. For the comparison among at least three groups, one-way analysis of variance (ANOVA) was applied. The Pearson test was used to analyze the correlation between miR-411-5p and LINC00461 and BNIP3."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets analyzed during the current study are available from the corresponding author upon request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35783530", "locator": "sections[23] \"Data Availability\" paragraph 2", "quote": "The datasets analyzed during the current study are available from the corresponding author upon request."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35783530"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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According to the groups, the transfected TMD8 cells were suspended with PBS in the de"], "order_basis": "paper_text_order:820", "id": "assay:1"}, {"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["to detect their effects on proliferation and apoptosis. Results: The level of LINC00461 in DLBCL wa", "function of LINC00461 was closely related to apoptosis. The results shown that miR-411-5p bound to", "461 inhibited cell proliferation and induced apoptosis. The inhibition of LINC00461 or overexpressi", "xpression of BNIP3 protein, thereby inducing apoptosis at the in vivo and in vitro levels. Conclusi"], "order_basis": "paper_text_order:931", "id": "assay:2"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["3 protein, thereby inducing apoptosis at the in vivo and in vitro levels. Conclusion: LINC00461 m", "eft armpit area of the mice. After 28 d, the mice were euthanized by cervical dislocation. During t"], "order_basis": "paper_text_order:1599", "id": "assay:3"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["miR-411-5p, and BNIP3 mRNA were detected by RT-qPCR. All patients were informed and agreed. This", "R-411-5p, and BNIP3 mRNA were detected by RT-qPCR. All patients were informed and agreed. This"], "order_basis": "paper_text_order:3897", "id": "assay:4"}, {"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Student's t-test was used for the comparison between two groups. For the comparison among at least three groups, one-way analysis of variance (ANOVA) was applied. The Pearson test was used to analyze the correlation between miR-411-5p and LINC00461 and BNIP3."], "order_basis": "consumes_earlier_output", "id": "assay:5"}]}