Nano pom-poms prepared exosomes enable highly specific cancer biomarker detection.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35787656", "kind": "science-record", "revision": 5, "content_hash": "sha256:e25c43561a6ac16336593332ab1d6da9facac4581f98a1294c4a4ef8d679c9fa", "created_at": "2026-11-01T18:14:49Z", "updated_at": "2026-11-01T22:49:27Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Nano pom-poms prepared exosomes enable highly specific cancer biomarker detection.", "doi": "10.1038/s42003-022-03598-0", "pmid": "35787656", "authors": ["Nan He", "Sirisha Thippabhotla", "Cuncong Zhong", "Zachary Greenberg", "Liang Xu", "Ziyan Pessetto", "Andrew K Godwin", "Yong Zeng", "Mei He"], "journal": "Communications biology", "year": 2022, "abstract": "Extracellular vesicles (EVs), particularly nano-sized small EV exosomes, are emerging biomarker sources. However, due to heterogeneous populations secreted from diverse cell types, mapping exosome multi-omic molecular information specifically to their pathogenesis origin for cancer biomarker identification is still extraordinarily challenging. Herein, we introduced a novel 3D-structured nanographene immunomagnetic particles (NanoPoms) with unique flower pom-poms morphology and photo-click chemistry for specific marker-defined capture and release of intact exosome. This specific exosome isolation approach leads to the expanded identification of targetable cancer biomarkers with enhanced specificity and sensitivity, as demonstrated by multi-omic exosome analysis of bladder cancer patient tissue fluids using the next generation sequencing of somatic DNA mutations, miRNAs, and the global proteome (Data are available via ProteomeXchange with identifier PXD034454). The NanoPoms prepared exosomes also exhibit distinctive in vivo biodistribution patterns, highlighting the highly viable and integral quality. The developed method is simple and straightforward, which is applicable to nearly all types of biological fluids and amenable for enrichment, scale up, and high-throughput exosome isolation.", "claims": [{"id": "claim-method", "statement": "The one-way ANOVA and t test were used.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35787656", "locator": "sections[17] \"Statistics and reproducibility\" paragraph 2", "quote": "The one-way ANOVA and t test were used."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All data generated or analyzed during this study are included in this published article (and its supplementary information file). Source data for the graphs and charts in the main figures is provided in the supplementary data files and any remaining information can be obtained from the corresponding author upon reasonable request. The sequence data are deposited in the Genbank nucleotide sequence database under Accession: PRJNA799354 with ID: 799354. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD034454 and 10.6019/PXD034454.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35787656", "locator": "sections[25] \"Data availability\" paragraph 2", "quote": "All data generated or analyzed during this study are included in this published article (and its supplementary information file). Source data for the graphs and charts in the main figures is provided in the supplementary data files and any remaining information can be obtained from the corresponding author upon reasonable request. The sequence data are deposited in the Genbank nucleotide sequence database under Accession: PRJNA799354 with ID: 799354. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD034454 and 10.6019/PXD034454."}], "state": "complete"}, {"id": "claim-target-result", "statement": "This specific exosome isolation approach leads to the expanded identification of targetable cancer biomarkers with enhanced specificity and sensitivity, as demonstrated by multi-omic exosome analysis of bladder cancer patient tissue fluids using the next generation sequencing of somatic DNA mutations, miRNAs, and the global proteome (Data are available via ProteomeXchange with identifier PXD034454).", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35787656", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "This specific exosome isolation approach leads to the expanded identification of targetable cancer biomarkers with enhanced specificity and sensitivity, as demonstrated by multi-omic exosome analysis of bladder cancer patient tissue fluids using the next generation sequencing of somatic DNA mutations, miRNAs, and the global proteome (Data are available via ProteomeXchange with identifier PXD034454)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "This specific exosome isolation approach leads to the expanded identification of targetable cancer biomarkers with enhanced specificity and sensitivity, as demonstrated by multi-omic exosome analysis of bladder cancer patient tissue fluids using the next generation sequencing of somatic DNA mutations, miRNAs, and the global proteome (Data are available via ProteomeXchange with identifier PXD034454).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35787656"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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The sequence data are deposited in the Genbank nucleotide sequence database under Accession: PRJNA799354 with ID: 799354. 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As shown in Fig. 5a, the Western blot bands from CD9 across all samples exhibited", "of bladder cancer patient urinary exosomes.a Western blotting analysis of urinary exosome proteins prepare"], "order_basis": "paper_text_order:12758", "id": "assay:3"}, {"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["The one-way ANOVA and t test were used."], "order_basis": "consumes_earlier_output", "id": "assay:4"}]}