Mitoribosome insufficiency in β cells is associated with type 2 diabetes-like islet failure.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35804190", "kind": "science-record", "revision": 5, "content_hash": "sha256:34c1dbbcef27b1017ea1e48c2b12a19edc9f7996103b16386fe84204f466a654", "created_at": "2026-11-01T18:14:51Z", "updated_at": "2026-11-01T22:49:30Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Mitoribosome insufficiency in β cells is associated with type 2 diabetes-like islet failure.", "doi": "10.1038/s12276-022-00797-x", "pmid": "35804190", "authors": ["Hyun Jung Hong", "Kyong Hye Joung", "Yong Kyung Kim", "Min Jeong Choi", "Seul Gi Kang", "Jung Tae Kim", "Yea Eun Kang", "Joon Young Chang", "Joon Ho Moon", "Sangmi Jun", "Hyun-Joo Ro", "Yujeong Lee", "Hyeongseok Kim", "Jae-Hyung Park", "Baeki E Kang", "Yunju Jo", "Heejung Choi", "Dongryeol Ryu", "Chul-Ho Lee", "Hail Kim", "Kyu-Sang Park", "Hyun Jin Kim", "Minho Shong"], "journal": "Experimental & molecular medicine", "year": 2022, "abstract": "Genetic variations in mitoribosomal subunits and mitochondrial transcription factors are related to type 2 diabetes. However, the role of islet mitoribosomes in the development of type 2 diabetes has not been determined. We investigated the effects of the mitoribosomal gene on β-cell function and glucose homeostasis. Mitoribosomal gene expression was analyzed in datasets from the NCBI GEO website (GSE25724, GSE76894, and GSE76895) and the European Nucleotide Archive (ERP017126), which contain the transcriptomes of type 2 diabetic and nondiabetic organ donors. We found deregulation of most mitoribosomal genes in islets from individuals with type 2 diabetes, including partial downregulation of CRIF1. The phenotypes of haploinsufficiency in a single mitoribosomal gene were examined using β-cell-specific Crif1 (Mrpl59) heterozygous-deficient mice. Crif1beta+/- mice had normal glucose tolerance, but their islets showed a loss of first-phase glucose-stimulated insulin secretion. They also showed increased β-cell mass associated with higher expression of Reg family genes. However, Crif1beta+/- mice showed earlier islet failure in response to high-fat feeding, which was exacerbated by aging. Haploinsufficiency of a single mitoribosomal gene predisposes rodents to glucose intolerance, which resembles the early stages of type 2 diabetes in humans.", "claims": [{"id": "claim-method", "statement": "We used an unpaired, two-tailed Student’s t test for comparisons between two groups and one-way ANOVA followed by Tukey’s HSD test for multiple comparisons between three or more groups.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35804190", "locator": "sections[13] \"Statistical analysis\" paragraph 2", "quote": "We used an unpaired, two-tailed Student’s t test for comparisons between two groups and one-way ANOVA followed by Tukey’s HSD test for multiple comparisons between three or more groups."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All data that support the findings of this study are available from the authors on reasonable request. No applicable resources were generated or analyzed during the current study.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35804190", "locator": "sections[30] \"notes\" paragraph 1", "quote": "All data that support the findings of this study are available from the authors on reasonable request. No applicable resources were generated or analyzed during the current study."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Haploinsufficiency of a single mitoribosomal gene predisposes rodents to glucose intolerance, which resembles the early stages of type 2 diabetes in humans.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35804190", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Haploinsufficiency of a single mitoribosomal gene predisposes rodents to glucose intolerance, which resembles the early stages of type 2 diabetes in humans."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "Haploinsufficiency of a single mitoribosomal gene predisposes rodents to glucose intolerance, which resembles the early stages of type 2 diabetes in humans.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35804190"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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