Low Expression of miR-491-3p Is Correlated with Lymph Node Metastasis in Gastric Cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35815280", "kind": "science-record", "revision": 4, "content_hash": "sha256:3d977145f53049beaae3d0f3847214a7f74c55b62f9e7124e9ee990e904edb6a", "created_at": "2026-11-01T18:14:53Z", "updated_at": "2026-11-01T22:07:00Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Low Expression of miR-491-3p Is Correlated with Lymph Node Metastasis in Gastric Cancer.", "doi": "10.1155/2022/7807956", "pmid": "35815280", "authors": ["Haiou Yu", "Shuang Luo"], "journal": "Evidence-based complementary and alternative medicine : eCAM", "year": 2022, "abstract": "Objective: MiR-491-3p, as a tumor suppressor miRNA, was found decreased in many solid tissues. In this study, we aim to investigate miR-491-3p expression in gastric cancer with or without lymph node metastasis (LNM).\nMethods: GSE173215 dataset from Gene Expression Omnibus (GEO) was used to measure miRNA expression from tissue samples of gastric cancer patients. Moreover, gastric tumor tissues (non-LNM: n = 78; LNM: n = 68) were obtained to detect the miR-491-3p expression. Receiver operating characteristic (ROC) curve and Kaplan-Meier (KM) survival analysis, as well as Cox regression analysis, were performed to reveal the role of miR-491-3p in diagnosis and prognosis of gastric cancer.\nResults: According to GSE173215 datasets (t = -11.25, adjust P value = 1.30E-06) and our clinical results (0.390 ± 0.193 vs. 0.562 ± 0.166, P < 0.005), the gastric cancer patients with LNM showed lower miR-491-3p expression than those without LNM, demonstrating a high diagnostic efficiency (sensitivity: 74.36%; specificity: 69.12%). In addition, both LNM and low miR-491-3p expression were correlated with the poor prognosis of gastric cancer. Furthermore, the LNM patient with low expression of miR-491-3p had the worse prognosis, but the non-LNM patient with high expression of miR-491-3p had the best prognosis. MiR-491-3p expression (HR = 0.003, 95%CI: 3.35E-04∼0.028) and LNM (HR = 2.326, 95%CI: 1.046∼5.173) were independent risk factors for gastric cancer.\nConclusion: Downregulated miR-491-3p expression was found in gastric cancer, being a high diagnostic efficiency and an independent risk factor for gastric cancer, especially in those having LNM.", "claims": [{"id": "claim-method", "statement": "The expressions of miRNAs detected using microarray assay (GSE173215 dataset from platform GPL25134) by Yu et al. revealed that LNM patients showed lower expressions of miR-491-3p, miR-6781-5p, and miR-3185 than non-LNM patients using GEO2R online software, especially miR-491-3p. Student's t-test, χ2 test, or Fisher's exact test were performed to compare differences between two groups as and when appropriate, and ANOVA analysis to compare differences among three groups followed by Tukey's test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35815280", "locator": "sections[10] \"4. Discussion\" paragraph 3", "quote": "The expressions of miRNAs detected using microarray assay (GSE173215 dataset from platform GPL25134) by Yu et al. revealed that LNM patients showed lower expressions of miR-491-3p, miR-6781-5p, and miR-3185 than non-LNM patients using GEO2R online software, especially miR-491-3p."}, {"asset": "source:pmid:35815280", "locator": "sections[7] \"2.5. Statistical Analysis\" paragraph 2", "quote": "Student's t-test, χ2 test, or Fisher's exact test were performed to compare differences between two groups as and when appropriate, and ANOVA analysis to compare differences among three groups followed by Tukey's test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data used to support the findings of this study are included within the article.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35815280", "locator": "sections[11] \"Data Availability\" paragraph 2", "quote": "The data used to support the findings of this study are included within the article."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Gastric cancer patients with LNM showed lower miR-491-3p expression than those without LNM, demonstrating a high diagnostic efficiency (sensitivity: 74.36%; specificity: 69.12%).", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35815280", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "According to GSE173215 datasets (t = −11.25, adjust P value = 1.30E-06) and our clinical results (0.390 ± 0.193 vs. 0.562 ± 0.166, P < 0.005), the gastric cancer patients with LNM showed lower miR-491-3p expression than those without LNM, demonstrating a high diagnostic efficiency (sensitivity: 74.36%; specificity: 69.12%)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "Gastric cancer patients with LNM showed lower miR-491-3p expression than those without LNM, demonstrating a high diagnostic efficiency (sensitivity: 74.36%; specificity: 69.12%).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35815280"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE173215"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "blocked", "blocked_on": "method:student_t", "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE173215"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": ["step:run_analysis blocked on method:student_t"]}, "data": {"state": "partial", "assets": [{"id": "source:pmid:35815280", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1155/2022/7807956", "sha256": "sha256:7f486e02f37d6f41b0ef2400445c5c76142de5299bfbd4ecef75b8c8aa794349", "bytes": 29223, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1155/2022/7807956", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC9262502", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE173215", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE173215", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE173215", "version": null, "note": "The data used to support the findings of this study are included within the article.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The data used to support the findings of this study are included within the article.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM5261950", "GSM5261951", "GSM5261952", "GSM5261953", "GSM5261954", "GSM5261955", "GSM5261956", "GSM5261957", "GSM5261958", "GSM5261959", "GSM5261960", "GSM5261961", "GSM5261962", "GSM5261963", "GSM5261964", "GSM5261965", "GSM5261966", "GSM5261967", "GSM5261968", "GSM5261969"], "sample_count": 20, "group_sizes": null, "group_sizes_basis": null, "platform": "25134", "taxon": "Homo sapiens", "title": "Differential expression of miRNA in tissue microarray", "resolution_checked_at": "2026-11-01T18:06:43Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:GSE173215 not downloaded", "asset:data:geo:GSE173215 licence not read"]}, "analysis": {"state": "partial", "methods": [{"id": "method-1", "family": "group_comparison", "specific": "student_t_test", "label": "Group comparison", "dsl_operations": [], "missing_operations": ["student_t"], "expressible": false, "parameters": {}, "data_shapes": ["two_or_more_groups_continuous"], "evidence": {"score": 1.0, "band": "reordered", "quote": "The expressions of miRNAs detected using microarray assay (GSE173215 dataset from platform GPL25134) by Yu et al. revealed that LNM patients showed lower expressions of miR-491-3p, miR-6781-5p, and miR-3185 than non-LNM patients using GEO2R online software, especially miR-491-3p. 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