The Mechanism of Wnt Pathway Regulated by Telocytes to Promote the Regeneration and Repair of Intrauterine Adhesions.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35844454", "kind": "science-record", "revision": 5, "content_hash": "sha256:b586a738310c195f9cebafc6dc05e5ac5aa1c50ec80fcf959ad16d43c524110f", "created_at": "2026-11-01T18:14:54Z", "updated_at": "2026-11-01T22:49:34Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "The Mechanism of Wnt Pathway Regulated by Telocytes to Promote the Regeneration and Repair of Intrauterine Adhesions.", "doi": "10.1155/2022/3809792", "pmid": "35844454", "authors": ["Cai-Zhen Zhao", "Ping Fu", "Wen-Ying Peng", "Na Pan", "Yuqiu Peng", "Min Zhao", "Weiquan Xie"], "journal": "Computational and mathematical methods in medicine", "year": 2022, "abstract": "Background: Telocytes (TCs), a novel interstitial cell type in the reproductive tract, participating in pathophysiology of intrauterine adhesions (IUA). This study further investigates the hypothesis that TCs, a source of Wnt, promote the regeneration and repair of IUA.\nMethods: RNA sequencing datasets of IUA patient (GSE160633) and mouse intestine mesenchymal cells (GSE94072) in GEO database were analyzed for differentially expressed genes (DEGs), and quantitative real-time PCR (qRT-PCR) measured indicated gene expression in TC-educated endometrial stromal cells (ESCs) and noneducated ESCs and verified the results of data mining from GEO database.\nResults: The expression levels of Wnt genes were downregulated in IUA compared to the control and were upregulated in TCs. In particular, the changes of Wnt5a expression level were the most significant (logFC = 4.0314 and adjusted P value = 0.0023), and the relative Wnt5a expression level was remarkably higher in TC-educated ESCs than noneducated ESCs verified by qRT-PCR (P = 0.0027).\nConclusions: TCs may enhance the regeneration and repair of IUA through the Wnt signaling pathway.", "claims": [{"id": "claim-method", "statement": "The R package “edgeR” program [45] was performed for differential expression analysis to select genes that had significant changes (adj.P.val < 0.05) with an absolute log2 fold change of 1 (upregulated genes) and −1 (downregulated genes) as screening threshold parameters, with focus on the genes in the Wnt signaling pathway, and the list of the Wnt-related genes was obtained from KEGG (Kyoto Encyclopedia of Genes and Genomes) database (https://www.kegg.jp/entry/map04310).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35844454", "locator": "sections[4] \"2.2. Differentially Expressed Genes (DEGs)\" paragraph 2", "quote": "The R package “edgeR” program [45] was performed for differential expression analysis to select genes that had significant changes (adj.P.val < 0.05) with an absolute log2 fold change of 1 (upregulated genes) and −1 (downregulated genes) as screening threshold parameters, with focus on the genes in the Wnt signaling pathway, and the list of the Wnt-related genes was obtained from KEGG (Kyoto Encyclopedia of Genes and Genomes) database (https://www.kegg.jp/entry/map04310)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "No data were used to support this study.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35844454", "locator": "sections[18] \"Data Availability\" paragraph 2", "quote": "No data were used to support this study."}], "state": "complete"}, {"id": "claim-target-result", "statement": "In particular, the changes of Wnt5a expression level were the most significant (logFC = 4.0314 and adjusted P value = 0.0023), and the relative Wnt5a expression level was remarkably higher in TC-educated ESCs than noneducated ESCs verified by qRT-PCR (P = 0.0027).", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35844454", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "In particular, the changes of Wnt5a expression level were the most significant (logFC = 4.0314 and adjusted P value = 0.0023), and the relative Wnt5a expression level was remarkably higher in TC-educated ESCs than noneducated ESCs verified by qRT-PCR (P = 0.0027)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "In particular, the changes of Wnt5a expression level were the most significant (logFC = 4.0314 and adjusted P value = 0.0023), and the relative Wnt5a expression level was remarkably higher in TC-educated ESCs than noneducated ESCs verified by qRT-PCR (P = 0.0027).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35844454"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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