Insulin-Like Growth Factor 2 mRNA-Binding Protein 3 and Its Related Molecules as Potential Biomarkers in Small-Cell Lung Cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35845940", "kind": "science-record", "revision": 5, "content_hash": "sha256:b46c0cb4ac6cc04f090f2dd2932ded1da17723f1cdd55465c5d73802cb2816da", "created_at": "2026-11-01T18:14:55Z", "updated_at": "2026-11-01T22:49:35Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Insulin-Like Growth Factor 2 mRNA-Binding Protein 3 and Its Related Molecules as Potential Biomarkers in Small-Cell Lung Cancer.", "doi": "10.1155/2022/5774339", "pmid": "35845940", "authors": ["Lin Zhu", "Xing Gao", "Yun Du", "Min Tang", "Xiaofei Guo", "Zhigang Chen", "Yulong Liu", "Yimin Lu"], "journal": "BioMed research international", "year": 2022, "abstract": "Background: Insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) plays a key role in tumorigenesis and tumor progression. Lung cancer is the leading cause of cancer-related death in men and women all over the world. However, the relationship between IGF2BP3 and small-cell lung cancer (SCLC) has not been reported yet.\nMethods: SCLC and normal samples (GSE19945 and GSE149507) were obtained in the Gene Expression Omnibus (GEO) dataset. Differential genes were screened by R software, and functional analysis and signal pathway enrichment analysis were carried out. In addition, we used the survival analysis database to analyze the relationship between prognosis and gene expression. Besides, immunohistochemistry (IHC) and quantitative real-time PCR (qPCR) were used for further research.\nResults: Five differentially expressed miRNAs and 9 differentially expressed mRNAs were selected by using R software. Survival analysis database results show that C7, CLIC5, PRDX1, IGF2BP3, and LDB2 were related the overall survival of patients with SCLC. Furthermore, multivariate analysis included that IGF2BP3 was independent risk factors for SCLC patients. Besides, gene function and signal pathway enrichment analysis showed that differentially expressed miRNAs were involved in the process of tumorigenesis and development. Furthermore, IHC and qPCR outcomes showed that the expression level of hsa-miR-182, hsa-miR-183, and IGF2BP3 was differentially expressed in normal lung tissues (NLTs) and SCLC tissues (SCLCTs).\nConclusions: Our results concluded that hsa-miR-182, hsa-miR-183, and IGF2BP3 may take part in the development of SCLC.", "claims": [{"id": "claim-method", "statement": "We utilized limma R package to screen differentially expressed genes. |log2FC| > 1 and FDR < 0.05 were set as standards.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35845940", "locator": "sections[4] \"2.2. Difference Analysis\" paragraph 2", "quote": "We utilized limma R package to screen differentially expressed genes. |log2FC| > 1 and FDR < 0.05 were set as standards."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35845940", "locator": "sections[22] \"Data Availability\" paragraph 2", "quote": "The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Our results concluded that hsa-miR-182, hsa-miR-183, and IGF2BP3 may take part in the development of SCLC.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35845940", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "Our results concluded that hsa-miR-182, hsa-miR-183, and IGF2BP3 may take part in the development of SCLC."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Our results concluded that hsa-miR-182, hsa-miR-183, and IGF2BP3 may take part in the development of SCLC.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35845940"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE149507"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {"fdr_threshold": "e with |log2FC| > 1 and FDR < 0", "log2fc_threshold": "limma R package with |log2FC| > 1 and FDR < 0"}, "inputs": ["data:geo:GSE149507"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:35845940", "role": "manuscript", "format": "xml", "media_type": 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"Homo sapiens", "title": "Microarray expression data of 18 pairs of small cell lung cancer (sclc) and tumor and adjacent lung tissues", "resolution_checked_at": "2026-11-01T18:06:43Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE19945", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE19945", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE19945", "version": null, "note": "The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets used and/or analyzed during the current study are available from the corresponding 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Besides, immunohistochemistry (IHC) and quantitative real-time PCR (qPCR)", "e expression. Besides, immunohistochemistry (IHC) and quantitative real-time PCR (qPCR) were", "tumorigenesis and development. Furthermore, IHC and qPCR outcomes showed that the expression"], "order_basis": "paper_text_order:787", "id": "assay:1"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["mistry (IHC) and quantitative real-time PCR (qPCR) were used for further research. Results: Fi", "enesis and development. Furthermore, IHC and qPCR outcomes showed that the expression level of", "immunohistochemistry (IHC) and quantitative real-time PCR (qPCR) were used for further research. Resul"], "order_basis": "paper_text_order:832", "id": "assay:2"}, {"role": "data_generation", "definition_id": "clinical_cohort", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["nd LDB2 were related the overall survival of patients with SCLC. Furthermore, multivariate analysis inc", "een Identified Genes and Overall Survival of Patients with SCLC KM plotter (http://kmplot.com/analysis/"], "order_basis": "paper_text_order:1120", "id": "assay:3"}, {"role": "analysis", "definition_id": "differential_expression", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["normalise_counts", "differential_expression_fit", "fdr_bh"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["We utilized limma R package to screen differentially expressed genes. |log2FC| > 1 and FDR < 0.05 were set as standards."], "order_basis": "consumes_earlier_output", "id": "assay:4"}]}