Identification of MEDAG and SERPINE1 Related to Hypoxia in Abdominal Aortic Aneurysm Based on Weighted Gene Coexpression Network Analysis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35874515", "kind": "science-record", "revision": 5, "content_hash": "sha256:0fcfa0bdb0df7cc9e34490997fef8112f9badcaf77d48ae01a22d600eaa8114e", "created_at": "2026-11-01T18:14:57Z", "updated_at": "2026-11-01T22:49:38Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification of MEDAG and SERPINE1 Related to Hypoxia in Abdominal Aortic Aneurysm Based on Weighted Gene Coexpression Network Analysis.", "doi": "10.3389/fphys.2022.926508", "pmid": "35874515", "authors": ["Biyun Teng", "Chaozheng Xie", "Yu Zhao", "Qiu Zeng", "Fangbiao Zhan", "Yangyang Feng", "Zhe Wang"], "journal": "Frontiers in physiology", "year": 2022, "abstract": "Purpose: Abdominal aortic aneurysm (AAA) is a severe cardiovascular disease that often results in high mortality due to sudden rupture. This paper aims to explore potential molecular mechanisms and effective targeted therapies to prevent and delay AAA rupture. Methods: We downloaded two microarray datasets (GSE98278 and GSE17901) from the Gene Expression Omnibus (GEO) database. Differential analysis and single-sample gene set enrichment analysis (ssGSEA) of hypoxia scores were performed on 48 AAA patients in GSE98278. We identified hypoxia- and ruptured AAA-related gene modules using weighted gene coexpression network analysis (WGCNA). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed using the R package clusterProfiler. For candidate genes, validation was conducted on the mouse dataset GSE17901. Finally, we predicted drug candidates associated with the hub genes using the HERB Chinese medicine database. Results: Eighty-two differentially expressed genes were screened in the ruptured and stable groups; 103 differentially expressed genes were identified between the high- and low-hypoxia groups; and WGCNA identified 58 differentially expressed genes. Finally, nine candidate genes were screened, including two hub genes (MEDAG and SERPINE1). We identified pathways such as cytokine-cytokine receptor interaction and T-helper 1-type immune response involved in AAA hypoxia and rupture. We predicted 93 traditional Chinese medicines (TCMs) associated with MEDAG and SERPINE1. Conclusion: We identified the hypoxic molecules MEDAG and SERPINE1 associated with AAA rupture. Our study provides an additional direction for the association between hypoxia and AAA rupture.", "claims": [{"id": "claim-method", "statement": "The R package limma (version 3.3.3) was used to identify differentially expressed genes (DEGs) between eAAA and rAAA and between the high and low hypoxia score groups.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35874515", "locator": "sections[6] \"Identification of Differentially Expressed Genes\" paragraph 2", "quote": "The R package limma (version 3.3.3) was used to identify differentially expressed genes (DEGs) between eAAA and rAAA and between the high and low hypoxia score groups."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data analyzed in the study were obtained from GEO, accession number: GSE98278 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE98278) and GSE17901 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE17901).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35874515", "locator": "sections[22] \"Data Availability Statement\" paragraph 2", "quote": "The data analyzed in the study were obtained from GEO, accession number: GSE98278 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE98278) and GSE17901 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE17901)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "We identified the hypoxic molecules MEDAG and SERPINE1 associated with AAA rupture.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35874515", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "We identified the hypoxic molecules MEDAG and SERPINE1 associated with AAA rupture."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "We identified the hypoxic molecules MEDAG and SERPINE1 associated with AAA rupture.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35874515"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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