Mechanism of Lysoforte in Improving Jejuna Morphology and Health in Broiler Chickens.

{"sci_version": "1.0", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35928108", "kind": "science-record", "revision": 3, "content_hash": "sha256:50005255b259d6d64b378d160b98d892f4998f2b9cd2c71a528df199a5b14f5c", "created_at": "2026-11-01T18:15:01Z", "updated_at": "2026-11-01T21:28:32Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Mechanism of Lysoforte in Improving Jejuna Morphology and Health in Broiler Chickens.", "doi": "10.3389/fvets.2022.946148", "pmid": "35928108", "authors": ["Xiaofeng Li", "Xiaoli Shi", "Noura M Mesalam", "Lei Liu", "Zhihao Chen", "Bing Yang"], "journal": "Frontiers in veterinary science", "year": 2022, "abstract": "Lysoforte (LFT) plays a vital role in maintaining broilers' health and intestinal morphology. However, the mechanism behind the effects of LFT improving intestinal morphology and health is still unclear. Therefore, this study was implemented to explore the central genes linked to the regulatory effect of LFT. Seventy-five newly hatched Cobb 500 male broilers were randomly divided into three groups: control, LFT500, and LFT1000 groups, with 25 chicks per group. The control chicks were provided with the basal diet, and the birds in LFT500 and LFT1000 groups were offered the same basal diet with 500 g/ton and 1,000 g/ton LFT, respectively. GSE94622 dataset consisted of the control and two LFT-treated groups (LFT500 and LFT1000). Jejuna samples were obtained from Gene Expression Omnibus (GEO). Totally 106-344 DEGs were obtained by comparing LFT500 and LFT1000 vs. control and LFT1000 vs. LFT500. Gene ontology (GO) enrichment suggested that the DEGs are mainly related to the phosphatidylethanolamine biosynthetic process and neuron projection extension. KEGG analysis suggested the DEGs were enriched in AGE-RAGE, fatty acid elongation, ECM-receptor interaction (ECMRI), glycerophospholipid metabolism, focal adhesion, unsaturated fatty acids biosynthesis, and ABC transporters. Moreover, 29 genes, such as REG4, GJB1, KAT2A, APOA5, SERPINE2, ELOVL1, ABCC2, ANKRD9, CYP4V2, and PISD, might be closely related to promoting jejuna morphology in broilers. Taken together, our observation enhances the understanding of LFT in maintaining intestinal architecture and the general health of broiler chickens.", "claims": [{"id": "claim-method", "statement": "To obtain the DEGs between the jejuna samples treated with and without LFT, GEO2R (http://www.ncbi.nlm.nih.gov/geo/geo2r) was used to analyze the data from GSE94622.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35928108", "locator": "sections[7] \"Data Processing\" paragraph 2", "quote": "To obtain the DEGs between the jejuna samples treated with and without LFT, GEO2R (http://www.ncbi.nlm.nih.gov/geo/geo2r) was used to analyze the data from GSE94622."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets presented in this study can be found in online repositories. 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The names of the repository/repositories and accession number(s) can be found in the article/Supplementary Material."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Moreover, 29 genes, such as REG4, GJB1, KAT2A, APOA5, SERPINE2, ELOVL1, ABCC2, ANKRD9, CYP4V2, and PISD, might be closely related to promoting jejuna morphology in broilers.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35928108", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Moreover, 29 genes, such as REG4, GJB1, KAT2A, APOA5, SERPINE2, ELOVL1, ABCC2, ANKRD9, CYP4V2, and PISD, might be closely related to promoting jejuna morphology in broilers."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Moreover, 29 genes, such as REG4, GJB1, KAT2A, APOA5, SERPINE2, ELOVL1, ABCC2, ANKRD9, CYP4V2, and PISD, might be closely related to promoting jejuna morphology in broilers.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35928108"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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