Six macrophage-associated genes in synovium constitute a novel diagnostic signature for osteoarthritis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35967352", "kind": "science-record", "revision": 4, "content_hash": "sha256:7bc0a727d40976c7f667e9e9dcf42ca737c31d1cbca1e79db5b72faee267209e", "created_at": "2026-11-01T18:15:05Z", "updated_at": "2026-11-01T22:07:16Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Six macrophage-associated genes in synovium constitute a novel diagnostic signature for osteoarthritis.", "doi": "10.3389/fimmu.2022.936606", "pmid": "35967352", "authors": ["Yiying Liu", "Taoyuan Lu", "Zaoqu Liu", "Wenhua Ning", "Siying Li", "Yanru Chen", "Xiaoyong Ge", "Chunguang Guo", "Youyang Zheng", "Xiangyang Wei", "Haiming Wang"], "journal": "Frontiers in immunology", "year": 2022, "abstract": "Background: Synovial macrophages play important roles in the formation and progression of osteoarthritis (OA). This study aimed to explore the biological and clinical significance of macrophage-associated genes (MAGs) in OA.\nMethods: The OA synovial gene expression profiles GSE89408 and GSE82107 were obtained from the GEO database. Single-sample gene set enrichment analysis (ssGSEA) and GSEA were employed to decipher differences in immune infiltration and macrophage-associated biological pathways, respectively. Protein-protein interaction (PPI) network analysis and machine learning were utilized to establish a macrophage-associated gene diagnostic signature (MAGDS). RT-qPCR was performed to test the expression of key MAGs in murine models.\nResults: OA synovium presented high levels of immune infiltration and activation of macrophage-associated biological pathways. A total of 55 differentially expressed MAGs were identified. Using PPI analysis and machine learning, a MAGDS consisting of IL1B, C5AR1, FCGR2B, IL10, IL6, and TYROBP was established for OA diagnosis (AUC = 0.910) and molecular pathological evaluation. Patients with high MAGDS scores may possess higher levels of immune infiltration and expression of matrix metalloproteinases (MMPs), implying poor biological alterations. The diagnostic value of MAGDS was also validated in an external cohort (AUC = 0.886). The expression of key MAGs was validated in a murine model using RT-qPCR. Additionally, a competitive endogenous RNA network was constructed to reveal the potential posttranscriptional regulatory mechanisms.\nConclusions: We developed and validated a MAGDS model with the ability to accurately diagnose and characterize biological alterations in OA. The six key MAGs may also be latent targets for immunoregulatory therapy.", "claims": [{"id": "claim-method", "statement": "The diagnostic value of the MAGDS and model genes was estimated via receiver operating characteristic (ROC) curve analysis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35967352", "locator": "sections[9] \"Establishment and validation of the MAGDS model\" paragraph 2", "quote": "The diagnostic value of the MAGDS and model genes was estimated via receiver operating characteristic (ROC) curve analysis."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/ Supplementary Material .", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35967352", "locator": "sections[23] \"Data availability statement\" paragraph 2", "quote": "The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/ Supplementary Material ."}], "state": "complete"}, {"id": "claim-target-result", "statement": "We developed and validated a MAGDS model with the ability to accurately diagnose and characterize biological alterations in OA.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35967352", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "We developed and validated a MAGDS model with the ability to accurately diagnose and characterize biological alterations in OA."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "We developed and validated a MAGDS model with the ability to accurately diagnose and characterize biological alterations in OA.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35967352"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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