EGR2 is a hub-gene in myocardial infarction and aggravates inflammation and apoptosis in hypoxia-induced cardiomyocytes.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35971091", "kind": "science-record", "revision": 5, "content_hash": "sha256:d03e30581d31da9c39c0c0d750b8a652db8d2b79b0d945a1e7014fdb1f24f5dd", "created_at": "2026-11-01T18:15:06Z", "updated_at": "2026-11-01T22:49:48Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "EGR2 is a hub-gene in myocardial infarction and aggravates inflammation and apoptosis in hypoxia-induced cardiomyocytes.", "doi": "10.1186/s12872-022-02814-3", "pmid": "35971091", "authors": ["Zhixiang Bo", "Shuwen Huang", "Li Li", "Lin Chen", "Ping Chen", "Xiaoyi Luo", "Fang Shi", "Bing Zhu", "Lin Shen"], "journal": "BMC cardiovascular disorders", "year": 2022, "abstract": "BACKGROUND: Myocardial infarction (MI) is characterized by coronary artery occlusion, ischemia and hypoxia of myocardial cells, leading to irreversible myocardial damage. Therefore, it is urgent to explore the potential mechanism of myocardial injury during the MI process to develop effective therapies for myocardial cell rescue.\nMETHODS: We downloaded the GSE71906 dataset from GEO DataSets, and the R software was used to identify the differentially expressed genes (DEGs) in mouse heart tissues of MI and sham controls. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment were performed to understand the significantly activated signaling pathways in MI. Protein-protein interaction (PPI) network was constructed to highlight the hub genes in DEGs. The Western Blot, qRT-PCR and TUNEL staining were used to explore the function of hub gene in hypoxia-induced cardiomyocytes in vitro.\nRESULTS: A total of 235 DEGs were identified in GSE71906 dataset. Functional enrichment analysis revealed that the upregulated genes were primarily associated with the inflammatory response and apoptosis. 20 hub genes were identified in PPI network, and the early growth response 2 (EGR2) was highlighted. In vitro. We confirmed the EGR2 was upregulated induced by hypoxia and revealed the upregulated EGR2 aggravates pro-inflammation and pro-apoptotic genes expression. In addition, EGR2 knockout mitigates hypoxia-induced inflammation and apoptosis in cardiomyocytes.\nCONCLUSION: The present study identified the EGR2 was a hub gene in myocardial damage during MI process, the excessive EGR2 aggravates hypoxia-induced myocardial damage by accelerating inflammation and apoptosis in vitro. Therefore, targeting EGR2 offers a potential pharmacological strategy for myocardial cell rescue in MI.", "claims": [{"id": "claim-method", "statement": "Significance was assessed by one-way ANOVA followed by Tukey’s test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35971091", "locator": "sections[13] \"Statistical analysis\" paragraph 2", "quote": "Significance was assessed by one-way ANOVA followed by Tukey’s test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used and/or analyzed during the current study are available from the GEO dataset. (Data link: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE71906).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35971091", "locator": "sections[28] \"notes\" paragraph 1", "quote": "The datasets used and/or analyzed during the current study are available from the GEO dataset. 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