Single-cell RNA sequencing to decipher the immunogenicity of ChAdOx1 nCoV-19/AZD1222 and mRNA-1273 vaccines in patients with autoimmune rheumatic diseases.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35979368", "kind": "science-record", "revision": 3, "content_hash": "sha256:69fb537dd7b7a6cfab055f15c9f3eb3609a5187ea4c4cff5ac8e7e8ed1e4c2d1", "created_at": "2026-11-01T18:15:06Z", "updated_at": "2026-11-01T21:28:39Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Single-cell RNA sequencing to decipher the immunogenicity of ChAdOx1 nCoV-19/AZD1222 and mRNA-1273 vaccines in patients with autoimmune rheumatic diseases.", "doi": "10.3389/fimmu.2022.920865", "pmid": "35979368", "authors": ["Yen-Ju Chen", "Po-Liang Cheng", "Wen-Nan Huang", "Hsin-Hua Chen", "Hong-Wei Chen", "Jun-Peng Chen", "Ching-Tsai Lin", "Kuo-Tung Tang", "Wei-Ting Hung", "Tsu-Yi Hsieh", "Yi-Hsing Chen", "Yi-Ming Chen", "Tzu-Hung Hsiao"], "journal": "Frontiers in immunology", "year": 2022, "abstract": "Objectives: To investigate the differences between the vector vaccine ChAdOx1 nCoV-19/AZD1222 (Oxford-AstraZeneca) and mRNA-based vaccine mRNA-1273 (Moderna) in patients with autoimmune rheumatic diseases (AIRD), and to explore the cell-cell interactions between high and low anti-SARS-CoV-2 IgG levels in patients with rheumatic arthritis (RA) using single-cell RNA sequencing (scRNA-seq).\nMethods: From September 16 to December 10, 2021, we consecutively enrolled 445 participants (389 patients with AIRD and 56 healthy controls), of whom 236 were immunized with AZD1222 and 209 with mRNA-1273. The serum IgG antibodies to the SARS-CoV-2 receptor-binding domain was quantified by electrochemiluminescence immunoassay at 4-6 weeks after vaccination. Moreover, peripheral blood mononuclear cells (PBMCs) were isolated from RA patients at 4-6 weeks after vaccination for scRNA-seq and further analyzed by CellChat. ScRNA-seq of PBMCs samples from GSE201534 in the Gene Expression Omnibus (GEO) database were also extracted for analysis.\nResults: The anti-SARS-CoV-2 IgG seropositivity rate was 85.34% for AIRD patients and 98.20% for healthy controls. The anti-SARS-CoV-2 IgG level was higher in patients receiving mRNA-1273 than those receiving AZD1222 (β: 35.25, 95% CI: 14.81-55.68, p=0.001). Prednisolone-equivalent dose >5 mg/day and methotrexate use in AIRD patients, and non-anti-tumor necrosis factor-α biologics and Janus kinase inhibitor use in RA patients were associated with inferior immunogenicity. ScRNA-seq revealed CD16-monocytes were predominant in RA patients with high anti-SARS-CoV2-IgG antibodies, and enriched pathways related to antigen presentation via MHC class II were found. HLA-DRA and CD4 interaction was enhanced in high anti-SARS-CoV2-IgG group.\nConclusions: mRNA-1273 and AZD1222 vaccines exhibited differential immunogenicity in AIRD patients. Enriched pathways related to antigen presentation via MHC class II in CD16-monocytes might be associated with higher anti-SARS-CoV2-IgG level in RA patients and further study is warranted.", "claims": [{"id": "claim-method", "statement": "Linear regression analyses were used to examine the immunogenicity and the impact of anti-rheumatic medications on immunogenicity in AIRD patients, expressed as regression coefficient (β) and 95% confidence intervals (95% CI).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35979368", "locator": "sections[11] \"Statistical analysis\" paragraph 2", "quote": "Linear regression analyses were used to examine the immunogenicity and the impact of anti-rheumatic medications on immunogenicity in AIRD patients, expressed as regression coefficient (β) and 95% confidence intervals (95% CI)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets presented in this study can be found in online repositories. 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