Novel lncRNA SNHG16 Promotes the Growth and Metastasis of Malignant Melanoma by Regulating miR-205-5p/PAK2 Axis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35983126", "kind": "science-record", "revision": 5, "content_hash": "sha256:957fd43d5842e8fbc158b9bca08fde939e934ab3a5e43f463444b41a7cc30b7d", "created_at": "2026-11-01T18:15:07Z", "updated_at": "2026-11-01T22:49:49Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Novel lncRNA SNHG16 Promotes the Growth and Metastasis of Malignant Melanoma by Regulating miR-205-5p/PAK2 Axis.", "doi": "10.2147/CCID.S374404", "pmid": "35983126", "authors": ["Yun Xia", "Jing Guan", "Xu Lu", "Yifan Liu", "Wenkang Luan"], "journal": "Clinical, cosmetic and investigational dermatology", "year": 2022, "abstract": "Background: Long non-coding RNAs (lncRNAs) play an key role in the biological processes of various malignant tumors. SNHG16 has been confirmed to be associated with the progression of various cancers. However, function and molecular mechanism of SNHG16 in melanoma have not been studied by scholars.\nMethods: The expression of SNHG16 in melanoma tissues were detected by using qRT-PCR. Melanoma cases from The Cancer Genome Atlas (TCGA) and GEO#GSE15605 were included in this study. CCK-8 assay, EdU assay, transwell and scratch wound assay were used to explore the role of SNHG16 in melanoma cells. Luciferase reporter assays and RNA pull-down assay were used to explore the molecular mechanism of SNHG16 in melanoma.\nResults: Here, we found that SNHG16 was up-regulated in melanoma. SNHG16 enhances the growth and metastasis of melanoma. SNHG16 can promote the expression of P21-activated kinases 2 (PAK2) by sponging miR-205-5p. PAK2 is the target gene of miR-205-5p. We demonstrated that SNHG16 promotes the metastasis and growth of melanoma through miR-205-5p/PAK2 axis.\nConclusion: This study firstly confirmed the role and mechanism of SNHG16 in the occurrence and development of melanoma. Therefore, SNHG16 may become a key point in the diagnosis, prognosis and treatment of melanoma patients in the future.", "claims": [{"id": "claim-method", "statement": "Whether the data have the statistically significant was estimated by t-test or one-way ANOVA. The Spearman correlation analysis was performed.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35983126", "locator": "sections[11] \"Statistical Analysis\" paragraph 2", "quote": "Whether the data have the statistically significant was estimated by t-test or one-way ANOVA. The Spearman correlation analysis was performed."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The human melanoma tissues comes from the Cancer Genome Atlas (TCGA, 461 melanoma tissues), and 558 normal skin tissue comes from GTEx database, We analyzed the relevant data by using the GEPIA website (http://gepia.cancer-pku.cn/index.html).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35983126", "locator": "sections[3] \"Human Tissues and Cells\" paragraph 2", "quote": "The human melanoma tissues comes from the Cancer Genome Atlas (TCGA, 461 melanoma tissues), and 558 normal skin tissue comes from GTEx database, We analyzed the relevant data by using the GEPIA website (http://gepia.cancer-pku.cn/index.html)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "SNHG16 promotes melanoma growth and metastasis through the miR-205-5p/PAK2 axis.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35983126", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "We demonstrated that SNHG16 promotes the metastasis and growth of melanoma through miR-205-5p/PAK2 axis."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "SNHG16 promotes melanoma growth and metastasis through the miR-205-5p/PAK2 axis.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35983126"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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