Exploring Key Genes and Pathways of Cardiac Hypertrophy Based on Bioinformatics.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36046382", "kind": "science-record", "revision": 4, "content_hash": "sha256:5a440f1d7a199f6ed4f6da28ea543d678d75249512ede7592ef8b72d17fa5d7a", "created_at": "2026-11-01T18:15:13Z", "updated_at": "2026-11-01T22:07:24Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Exploring Key Genes and Pathways of Cardiac Hypertrophy Based on Bioinformatics.", "doi": "10.1155/2022/2081590", "pmid": "36046382", "authors": ["Zhenzhen Zhang", "Chunxiao Wang"], "journal": "Disease markers", "year": 2022, "abstract": "Objective: This research is aimed at identifying the key genes and pathways of cardiac hypertrophy using bioinformatics and at providing a new target for the identification of cardiac hypertrophy.\nMethods: Microarray data GSE1621 and GSE18801 were acquired from the GEO database. The DEGs of GSE1621 and GSE18801 were analyzed using the online tool GEO2R. \"ggplot2\" package of R software was utilized to generate the volcano plots. The top and bottom 10 genes were mapped as a heat map. GO functional annotation analysis and KEGG pathway enrichment analysis were performed separately for DEGs using the online software DAVID. Histograms were plotted using the R \"ggplot2\" package. The DEGs were imported into the STRING online database for constructing PPI networks and analyzing the DEG interaction relationships.\nResults: In the present study, 469 DEGs were screened in GSE1621 and a total of 793 DEGs were screened in GSE18801. GO analyses indicate that DEGs were mainly involved in cardiac muscle contraction, regulation of blood circulation, regulation of muscle contraction, muscle contraction, striated muscle contraction, regulation of heart contraction, regulation of striated muscle contraction, and tissue remodeling. KEGG analyses indicate that DEGs were mainly involved in Th17 cell differentiation, Th1 and Th2 cell differentiation, HIF-1 signaling pathway, pathways in cancer, hematopoietic cell lineage, Chagas disease and cell adhesion molecules, viral myocarditis, central carbon metabolism in cancer, acute myeloid leukemia, and JAK-STAT signaling pathway. Eight hub genes were screened, including Akt1, Lox, Timp1, Col1al, Spp1, Ccnd1, Mmp3, and Egfr.\nConclusions: The DEGs associated with cardiac hypertrophy were screened via bioinformatics analysis, and eight hub genes were identified, including Akt1, Lox, Timp1, Col1al, Spp1, Ccnd1, Mmp3, and Egfr, which might be a new target for the identification of cardiac hypertrophy.", "claims": [{"id": "claim-method", "statement": "The DEGs of GSE1621 and GSE18801 were analyzed using the online tool GEO2R. The screening criteria for DEGs were adjusted P value < 0.05 and |Log2 fold change (LogFC)| >1.5.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36046382", "locator": "sections[4] \"2.2. Identification of DEGs\" paragraph 2", "quote": "The DEGs of GSE1621 and GSE18801 were analyzed using the online tool GEO2R. The screening criteria for DEGs were adjusted P value < 0.05 and |Log2 fold change (LogFC)| >1.5."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data to support the findings of this study is available on reasonable request from the corresponding author.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36046382", "locator": "sections[13] \"Data Availability\" paragraph 2", "quote": "Data to support the findings of this study is available on reasonable request from the corresponding author."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The intersection analysis identified 38 co-DEGs between GSE1621 and GSE18801, comprising 17 upregulated and 21 downregulated genes.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36046382", "locator": "sections[8] \"3.1. Identification of DEGs\" paragraph 2", "quote": "38 co-DEGs, including 17 upregulated genes (Figure 2(c)) and 21 downregulated genes (Figure 2(d)), were obtained by taking the intersection from DEGs of GSE1621 and GSE18801."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The intersection analysis identified 38 co-DEGs between GSE1621 and GSE18801, comprising 17 upregulated and 21 downregulated genes.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36046382"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE1621"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE1621"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:36046382", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1155/2022/2081590", "sha256": "sha256:f5a2fb9e02cb158d50a06535c770c802b132c8d980634c21f4ecbc7ce76994c2", "bytes": 21974, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1155/2022/2081590", "version": null, "note": "Full article fetched from PMC; 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The screening criteria for DEGs were adjusted P value < 0.05 and |Log2 fold change (LogFC)| >1.5."}}], "targets": [], "gaps": ["method.reader_named_unmapped"]}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "complete", "tier": "R1", "blocking_axis": "none", "blocked_on": "nothing:replicable_now", "axes": {"method": {"tier": "R1", "expressible": true, "required_operations": ["build_graph", "centrality_scores", "pathway_enrich"], "missing_operations": [], "basis": "The paper names the method it used and the corpus's method extractor maps it onto the DSL catalogue: ppi_network."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. 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