Construction and evaluation of a prognostic risk model of tumor metastasis-related genes in patients with non-small cell lung cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36056349", "kind": "science-record", "revision": 4, "content_hash": "sha256:aba2a20c9efa529e43296eea4143c345f3c3f321ae17da263565b4d2a6e1e5d9", "created_at": "2026-11-01T18:15:14Z", "updated_at": "2026-11-01T22:07:26Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Construction and evaluation of a prognostic risk model of tumor metastasis-related genes in patients with non-small cell lung cancer.", "doi": "10.1186/s12920-022-01341-6", "pmid": "36056349", "authors": ["Huan Ding", "Li Shi", "Zhuo Chen", "Yi Lu", "Zhiyu Tian", "Hongyu Xiao", "Xiaojing Deng", "Peiyi Chen", "Yue Zhang"], "journal": "BMC medical genomics", "year": 2022, "abstract": "BACKGROUND: Lung cancer is a high-incidence cancer, and it is also the most common cause of cancer death worldwide. 80-85% of lung cancer cases can be classified as non-small cell lung cancer (NSCLC).\nMETHODS: NSCLC transcriptome data and clinical information were downloaded from the TCGA database and GEO database. Firstly, we analyzed and identified the differentially expressed genes (DEGs) between non-metastasis group and metastasis group of NSCLC in the TCGA database, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) were consulted to explore the functions of the DEGs. Thereafter, univariate Cox regression and LASSO Cox regression algorithms were applied to identify prognostic metastasis-related signature, followed by the construction of the risk score model and nomogram for predicting the survival of NSCLC patients. GSEA analyzed that differentially expressed gene-related signaling pathways in the high-risk group and the low-risk group. The survival of NSCLC patients was analyzed by the Kaplan-Meier method. ROC curve was plotted to evaluate the accuracy of the model. Finally, the GEO database was further applied to verify the metastasis‑related prognostic signature.\nRESULTS: In total, 2058 DEGs were identified. GO functions and KEGG pathways analysis results showed that the DEGs mainly concentrated in epidermis development, skin development, and the pathway of Neuro active ligand -receptor interaction in cancer. A six-gene metastasis-related risk signature including C1QL2, FLNC, LUZP2, PRSS3, SPIC, and GRAMD1B was constructed to predict the overall survival of NSCLC patients. The reliability of the gene signature was verified in GSE13213. The NSCLC patients were grouped into low-risk and high-risk groups based on the median value of risk scores. And low-risk patients had lower risk scores and longer survival time. Univariate and multivariate Cox regression verified that this signature was an independent risk factor for NSCLC.\nCONCLUSION: Our study identified 6 metastasis biomarkers in the NSCLC. The biomarkers may contribute to individual risk estimation, survival prognosis.", "claims": [{"id": "claim-method", "statement": "Thereafter, univariate Cox regression and LASSO Cox regression algorithms were applied to identify prognostic metastasis-related signature, followed by the construction of the risk score model and nomogram for predicting the survival of NSCLC patients.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36056349", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "Thereafter, univariate Cox regression and LASSO Cox regression algorithms were applied to identify prognostic metastasis-related signature, followed by the construction of the risk score model and nomogram for predicting the survival of NSCLC patients."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets generated and/or analysed during the current study are available in the TCGA repository. The Cancer Genome Atlas Program—National Cancer Institute. The validation set from GEO database, the accession number of GEO datasets is GSE13213. GEO Accession viewer (nih.gov).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36056349", "locator": "sections[23] \"Availability of data and materials\" paragraph 2", "quote": "The datasets generated and/or analysed during the current study are available in the TCGA repository. The Cancer Genome Atlas Program—National Cancer Institute. The validation set from GEO database, the accession number of GEO datasets is GSE13213. 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This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE13213"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE13213"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:36056349", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1186/s12920-022-01341-6", "sha256": "sha256:866612a894ea52ab34f382410aef8d349dfa142be1af763122c11995a466f179", "bytes": 38863, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1186/s12920-022-01341-6", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC9440521", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE13213", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE13213", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE13213", "version": null, "note": "The datasets generated and/or analysed during the current study are available in the TCGA repository. 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