Bruton tyrosine kinase (BTK) may be a potential therapeutic target for interstitial cystitis/bladder pain syndrome.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36069808", "kind": "science-record", "revision": 5, "content_hash": "sha256:d6a7ba803312e8e265cfed45c7f6715fc5d68c0ec48861af26029b7d31eeb34f", "created_at": "2026-11-01T18:15:17Z", "updated_at": "2026-11-01T22:50:00Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Bruton tyrosine kinase (BTK) may be a potential therapeutic target for interstitial cystitis/bladder pain syndrome.", "doi": "10.18632/aging.204271", "pmid": "36069808", "authors": ["Guang Wang", "Tong-Xin Yang", "Jiong-Ming Li", "Zi-Ye Huang", "Wen-Bo Yang", "Pei Li", "Da-Lin He"], "journal": "Aging", "year": 2022, "abstract": "AIMS: To determine the potential diagnostic and therapeutic targets of Interstitial Cystitis/Bladder Pain Syndrome (IC/BPS).\nMETHODS: We selected the GSE11783, GSE57560 and GSE621 datasets from the GEO database and merged them. R software was used to screen differentially expressed genes (DEGs) between IC/BPS and normal bladder tissues. The \"String\" online tool is used to analyze DEGs interaction and functional protein enrichment. CIBERSORT online tool was used to analyze the infiltration of immune cells. In addition, we verified the function of BTK in IC/BPS at the clinical samples and cells level.\nRESULTS: Bioinformatics analysis revealed that 5 genes were significantly overexpressed in IC/BPS, and the protein-protein interaction diagram showed that BTK was a critical link between these five proteins. At the same time, functional enrichment showed that they were significantly related to innate immunity. Immunoinfiltration showed that mast cell resting in IC/BPS was significantly higher. IHC staining of clinical samples showed that the mast cell markers Tryptase and BTK were highly expressed in IC/BPS tissues. At the cell level, knockdown of BTK inhibited proliferation, migration, invasion, and degranulation of mast cells.\nCONCLUSIONS: This study provides a new perspective for understanding the molecular mechanisms involved in IC/BPS and suggests that BTK may be a target for treating IC/BPS.", "claims": [{"id": "claim-method", "statement": "The “limma” package analyzed the DEGs, and the adjusted P-value <0.05 and absolute logFC (Fold change)>1 was used as the screening criteria for DEGs.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36069808", "locator": "sections[12] \"DEGs analysis of IC/BPS GEO datasets\" paragraph 2", "quote": "The “limma” package analyzed the DEGs, and the adjusted P-value <0.05 and absolute logFC (Fold change)>1 was used as the screening criteria for DEGs."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The GSE11783, GSE57560, and GSE621 datasets generated and/or analysed during the current study are available in the GEO database repository, (https://www.ncbi.nlm.nih.gov/guide/genes-expression/). And original photographs of Western blots in Figures 4E, 5A, and sh-BTK and NC sequences are shown in Supplementary Figures 1, 2.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36069808", "locator": "sections[23] \"Data availability\" paragraph 2", "quote": "The GSE11783, GSE57560, and GSE621 datasets generated and/or analysed during the current study are available in the GEO database repository, (https://www.ncbi.nlm.nih.gov/guide/genes-expression/). And original photographs of Western blots in Figures 4E, 5A, and sh-BTK and NC sequences are shown in Supplementary Figures 1, 2."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Bioinformatics analysis revealed that 5 genes were significantly overexpressed in IC/BPS, and the protein-protein interaction diagram showed that BTK was a critical link between these five proteins.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36069808", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "Bioinformatics analysis revealed that 5 genes were significantly overexpressed in IC/BPS, and the protein-protein interaction diagram showed that BTK was a critical link between these five proteins."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Bioinformatics analysis revealed that 5 genes were significantly overexpressed in IC/BPS, and the protein-protein interaction diagram showed that BTK was a critical link between these five proteins.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36069808"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE11783"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE11783"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:36069808", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.18632/aging.204271", "sha256": "sha256:351270ec2c04c624740f95ef488df8585f498b53a7699885b1d6179cafa39db3", "bytes": 37049, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.18632/aging.204271", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC9512503", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE11783", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11783", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE11783", "version": null, "note": "The GSE11783, GSE57560, and GSE621 datasets generated and/or analysed during the current study are available in the GEO database repository, (https://www.ncbi.nlm.nih.gov/guide/genes-expression/). 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IHC staining of clinical samples showed that the", "r of mast cells, in the samples collected by IHC. The results showed that compared with NC gr", "fore, we chose BTK in DEGs for verification. IHC staining showed that the positive rate of BT", "ly reduced. Scale bar: 100 and 50 μm. (C, D) IHC staining showed the difference of (C) Trypta"], "order_basis": "paper_text_order:1076", "id": "assay:1"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.85, "evidence": ["ressed in IC/BPS tissues. At the cell level, knockdown of BTK inhibited proliferation, migration, i"], "order_basis": "paper_text_order:1219", "id": "assay:2"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["igher than that in NC group (Figure 4D). The Western blotting also got consistent results (Figure 4E). It", "al and IC/BPS tissues. Scale bar: 20 μm. (E) Western blotting exhibited the expression level of BTK in nor"], "order_basis": "paper_text_order:3374", "id": "assay:3"}, {"role": "analysis", "definition_id": "differential_expression", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["normalise_counts", "differential_expression_fit", "fdr_bh"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["The “limma” package analyzed the DEGs, and the adjusted P-value <0.05 and absolute logFC (Fold change)>1 was used as the screening criteria for DEGs."], "order_basis": "consumes_earlier_output", "id": "assay:4"}]}