Identification of Signal Pathways and Hub Genes of Pulmonary Arterial Hypertension by Bioinformatic Analysis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36072642", "kind": "science-record", "revision": 5, "content_hash": "sha256:82c45993cf9ba0f3e2ffad7ae8b32b0424ffa67722e6c00aea09a93d3b728ab9", "created_at": "2026-11-01T18:15:17Z", "updated_at": "2026-11-01T22:50:01Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification of Signal Pathways and Hub Genes of Pulmonary Arterial Hypertension by Bioinformatic Analysis.", "doi": "10.1155/2022/1394088", "pmid": "36072642", "authors": ["Rui-Qi Wei", "Wen-Mei Zhang", "Zhe Liang", "Chunmei Piao", "Guangfa Zhu"], "journal": "Canadian respiratory journal", "year": 2022, "abstract": "Pulmonary arterial hypertension (PAH) is a progressive and complex pulmonary vascular disease with poor prognosis. The aim of this study was to provide a new understanding of the pathogenesis of disease and potential treatment targets for patients with PAH based on multiple-microarray analysis.Two microarray datasets (GSE53408 and GSE113439) downloaded from the Gene Expression Omnibus (GEO) database were analysed. All the raw data were processed by R, and differentially expressed genes (DEGs) were screened out by the \"limma\" package. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed and visualized by R and Cytoscape software. Protein-protein interactions (PPI) of DEGs were analysed based on the NetworkAnalyst online tool. A total of 442 upregulated DEGs and 84 downregulated DEGs were identified. GO enrichment analysis showed that these DEGs were mainly enriched in mitotic nuclear division, organelle fission, chromosome segregation, nuclear division, and sister chromatid segregation. Significant KEGG pathway enrichment included ribosome biogenesis in eukaryotes, RNA transport, proteoglycans in cancer, dilated cardiomyopathy, rheumatoid arthritis, vascular smooth muscle contraction, focal adhesion, regulation of the actin cytoskeleton, and hypertrophic cardiomyopathy. The PPI network identified 10 hub genes including HSP90AA1, CDC5L, MDM2, LRRK2, CFTR, IQGAP1, CAND1, TOP2A, DDX21, and HIF1A. We elucidated potential biomarkers and therapeutic targets for PAH by bioinformatic analysis, which provides a theoretical basis for future study.", "claims": [{"id": "claim-method", "statement": "We used the “limma” package [13] for DEG analysis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36072642", "locator": "sections[5] \"2.3. Screening and Integration of DEGs\" paragraph 2", "quote": "We used the “limma” package [13] for DEG analysis."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All data relevant to the study are included in the article and are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36072642", "locator": "sections[17] \"Data Availability\" paragraph 2", "quote": "All data relevant to the study are included in the article and are available from the corresponding author on reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study identified 442 upregulated and 84 downregulated differentially expressed genes in pulmonary arterial hypertension using integrated analysis of two microarray datasets.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36072642", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "A total of 442 upregulated DEGs and 84 downregulated DEGs were identified."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study identified 442 upregulated and 84 downregulated differentially expressed genes in pulmonary arterial hypertension using integrated analysis of two microarray datasets.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36072642"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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