Identification of a Signature for Predicting Prognosis and Immunotherapy Response in Patients with Glioma.

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In the present study, gene expression profiles of GSE2223 were obtained from the Gene Expression Omnibus (GEO) database. Core modules and hub genes related to glioma were identified using weighted gene coexpression network analysis (WGCNA) and protein-protein interaction (PPI) network analysis of differentially expressed genes (DEGs). After a series of database screening tests, we identified 11 modules during glioma progression, followed by six hub genes (RAB3A, TYROBP, SYP, CAMK2A, VSIG4, and GABRA1) that can predict the prognosis of glioma and were validated in glioma tissues by qRT-PCR. The CIBERSORT algorithm was used to analyze the difference of immune cell infiltration between the glioma and control groups. Finally, Identification VSIG4 for immunotherapy response in patients with glioma demonstrating utility for immunotherapy research.", "claims": [{"id": "claim-method", "statement": "The Kaplan–Meier curve of GEPIA [15] was used to analyze the overall survival (OS) and disease-free survival (DFS) for hub genes in TCGA glioma patients.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36072978", "locator": "sections[8] \"2.6. Survival Analysis\" paragraph 2", "quote": "The Kaplan–Meier curve of GEPIA [15] was used to analyze the overall survival (OS) and disease-free survival (DFS) for hub genes in TCGA glioma patients."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Publicly available datasets were analyzed in this study. These data can be found in the following link: https://www.ncbi.nlm.nih.gov/sra/?term=SRP049695.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36072978", "locator": "sections[26] \"Data Availability\" paragraph 2", "quote": "Publicly available datasets were analyzed in this study. These data can be found in the following link: https://www.ncbi.nlm.nih.gov/sra/?term=SRP049695."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Therefore, the six hub genes had a significant prognostic value in glioma patients and predicted the OS rate and progression-free interval (PFI).", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36072978", "locator": "sections[19] \"3.7. 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