Comprehensive analysis of potential ceRNA network and immune cell infiltration in intervertebral disc degeneration.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36175893", "kind": "science-record", "revision": 5, "content_hash": "sha256:f05ec1bd826df3a19829600beed89e99111a936ada2a6c34116723206f534462", "created_at": "2026-11-01T18:15:27Z", "updated_at": "2026-11-01T22:50:11Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Comprehensive analysis of potential ceRNA network and immune cell infiltration in intervertebral disc degeneration.", "doi": "10.1186/s13018-022-03331-x", "pmid": "36175893", "authors": ["Xiaoyang Fang", "Tian Tang", "Daoxi Sun", "Shuang Chen", "Nan Wang", "Lin Xie"], "journal": "Journal of orthopaedic surgery and research", "year": 2022, "abstract": "BACKGROUND: Intervertebral disc degeneration (IDD) has become a serious public health problem, the mechanism of which is complex and still unclear. We aimed to construct a ceRNA network related to IDD to explore its pathogenesis.\nMETHODS: We downloaded the GSE67566, GSE63492, GSE116726 and GSE124272 datasets from GEO database, and obtained the differentially expressed RNAs. Then, we constructed a ceRNA network and the KEGG and GO enrichment analysis were performed. Finally, we performed immune cell infiltration analysis on the GSE124272 dataset and analysed the correlation between immune cell abundance and hub genes expression levels.\nRESULTS: The ceRNA network included three down-regulated circRNAs: hsa_circ_0074817, hsa_circ_0002702, hsa_circ_0003600, three up-regulated miRNAs: hsa-miR-4741, hsa-miR-3158-5p, hsa-miR-508-5p, and 57 down-regulated mRNAs, including six hub genes: IGF1, CHEK1, CCNB1, OIP5, BIRC5, AR. GO and KEGG analysis revealed that the network is involved in various biological functions. Immune infiltration analysis showed that IDD was closely related to immune cell infiltration, and hub genes could further affect the development of IDD by affecting immune cell infiltration.\nCONCLUSION: This study identified the hsa_circ_0074817-hsa-miR-508-5p-IGF1/CHEK1/CCNB1, the hsa_circ_0003600-hsa-miR-4741-BIRC5/OIP5/AR and the hsa_circ_0002702-hsa-miR-3158-5p-IGF1/AR as important regulatory axis of IDD, which will help us gain further insight into the pathogenesis of IDD and determine potential therapeutic targets.", "claims": [{"id": "claim-method", "statement": "Using the R limma package to convert probe IDs and setting “p value < 0.05 and |logFC|> 1” to obtain differentially expressed circRNAs(DEcircRNAs)、differentially expressed miRNAs(DEmiRNAs) and differentially expressed mRNAs(DEmRNAs).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36175893", "locator": "sections[4] \"Identification of differentially expressed RNA\" paragraph 2", "quote": "Using the R limma package to convert probe IDs and setting “p value < 0.05 and |logFC|> 1” to obtain differentially expressed circRNAs(DEcircRNAs)、differentially expressed miRNAs(DEmiRNAs) and differentially expressed mRNAs(DEmRNAs)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36175893", "locator": "sections[22] \"notes\" paragraph 1", "quote": "The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "This study identified the hsa_circ_0074817-hsa-miR-508-5p-IGF1/CHEK1/CCNB1, the hsa_circ_0003600-hsa-miR-4741-BIRC5/OIP5/AR and the hsa_circ_0002702-hsa-miR-3158-5p-IGF1/AR as important regulatory axis of IDD, which will help us gain further insight into the pathogenesis of IDD and determine potential therapeutic targets.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36175893", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "This study identified the hsa_circ_0074817-hsa-miR-508-5p-IGF1/CHEK1/CCNB1, the hsa_circ_0003600-hsa-miR-4741-BIRC5/OIP5/AR and the hsa_circ_0002702-hsa-miR-3158-5p-IGF1/AR as important regulatory axis of IDD, which will help us gain further insight into the pathogenesis of IDD and determine potential therapeutic targets."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "This study identified the hsa_circ_0074817-hsa-miR-508-5p-IGF1/CHEK1/CCNB1, the hsa_circ_0003600-hsa-miR-4741-BIRC5/OIP5/AR and the hsa_circ_0002702-hsa-miR-3158-5p-IGF1/AR as important regulatory axis of IDD, which will help us gain further insight into the pathogenesis of IDD and determine potential therapeutic targets.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36175893"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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