A New Monoclonal Antibody Enables BAR Analysis of Subcellular Importin β1 Interactomes.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36180036", "kind": "science-record", "revision": 4, "content_hash": "sha256:85a4fe651ac4e6abfaa0ebca079490f2f638e6068198f50040fbd342cdfba160", "created_at": "2026-11-01T18:15:28Z", "updated_at": "2026-11-01T22:07:40Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "A New Monoclonal Antibody Enables BAR Analysis of Subcellular Importin β1 Interactomes.", "doi": "10.1016/j.mcpro.2022.100418", "pmid": "36180036", "authors": ["Didi-Andreas Song", "Stefanie Alber", "Ella Doron-Mandel", "Vera Schmid", "Christin A Albus", "Orith Leitner", "Hedva Hamawi", "Juan A Oses-Prieto", "Nili Dezorella", "Alma L Burlingame", "Mike Fainzilber", "Ida Rishal"], "journal": "Molecular & cellular proteomics : MCP", "year": 2022, "abstract": "Importin β1 (KPNB1) is a nucleocytoplasmic transport factor with critical roles in both cytoplasmic and nucleocytoplasmic transport, hence there is keen interest in the characterization of its subcellular interactomes. We found limited efficiency of BioID in the detection of importin complex cargos and therefore generated a highly specific and sensitive anti-KPNB1 monoclonal antibody to enable biotinylation by antibody recognition analysis of importin β1 interactomes. The monoclonal antibody recognizes an epitope comprising residues 301-320 of human KPBN1 and strikingly is highly specific for cytoplasmic KPNB1 in diverse applications, with little reaction with KPNB1 in the nucleus. Biotinylation by antibody recognition with this novel antibody revealed numerous new interactors of importin β1, expanding the KPNB1 interactome to cytoplasmic and signaling complexes that highlight potential new functions for the importins complex beyond nucleocytoplasmic transport. Data are available via ProteomeXchange with identifier PXD032728.", "claims": [{"id": "claim-method", "statement": "To assess the statistical significance of protein fold changes between treatment conditions, log2 fold change ratios were averaged across replicates and p-values were calculated using Prism software (GraphPad) applying multiple unpaired t test (two-stage step-up Benjamini, Krieger, and Yekutieli (40)) with a desired false discovery rate of 10%.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36180036", "locator": "sections[23] \"Experimental Design and Statistical Rationale\" paragraph 2", "quote": "To assess the statistical significance of protein fold changes between treatment conditions, log2 fold change ratios were averaged across replicates and p-values were calculated using Prism software (GraphPad) applying multiple unpaired t test (two-stage step-up Benjamini, Krieger, and Yekutieli (40)) with a desired false discovery rate of 10%."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE (57) partner repository with the dataset identifier PXD032728. 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