Identification of Diagnostic Exosomal LncRNA-miRNA-mRNA Biomarkers in Colorectal Cancer Based on the ceRNA Network.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36185995", "kind": "science-record", "revision": 4, "content_hash": "sha256:dc0892b85d11bd315ca10aed824d00ebf312da898c356fd01087458099cf4eb9", "created_at": "2026-11-01T18:15:28Z", "updated_at": "2026-11-01T22:07:41Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification of Diagnostic Exosomal LncRNA-miRNA-mRNA Biomarkers in Colorectal Cancer Based on the ceRNA Network.", "doi": "10.3389/pore.2022.1610493", "pmid": "36185995", "authors": ["Yajing Zhao", "Xingguo Song", "Xianrang Song", "Li Xie"], "journal": "Pathology oncology research : POR", "year": 2022, "abstract": "Background: Colorectal cancer (CRC) is currently the fourth most common cancer worldwide. The roles of exosomal competing endogenous RNAs (ceRNAs) in CRC remain unclear. In this study, we constructed an exosomal ceRNA network to identify the core ceRNAs and investigate the diagnostic biomarkers in CRC. Methods and Patients: Serum exosomes were isolated from four CRC patients and two healthy donors by ultracentrifugation, and then subjected to RNA isolation, sequencing and microarray. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) analyses were performed to identify functional enrichment implications of differentially expressed exosomal mRNAs. TargetScan and miRanda were used for identifying the miRNA-mRNA and miRNA-LncRNA interactions. The predicted lncRNAs and mRNAs were intersected with the differentially expressed genes, for which the screening criterion was fold change >1.5 in the microarray. Differentially expressed exosomal miRNAs were identified in the GSE71008 dataset, and differentially expressed mRNAs (DEmRNAs) were further summarized from The Cancer Genome Atlas (TCGA) database. Results: A total of 1186 exosomal DEmRNAs, 2088 exosomal DElncRNAs and 29 exosomal miRNAs were detected in CRC patients compared to the healthy donors. Functional enrichment analysis suggested that exosomal DEmRNAs might participate in pathways related to carcinogenesis and development of cancer. An exosomal ceRNA regulatory network of CRC was constructed based on 40 lncRNAs, two miRNAs, and five mRNAs. Exosomal miR-150-5p and miR-10b-5p expression levels were increased in healthy donors compared with CRC patients in the GSE71008 dataset, and five DEmRNAs (TOMM70A, RBM48, BEND3, RHOBTB1, and ADAMTS2) were significantly upregulated in TCGA database. Two potential exosomal regulatory axes of lncRNA G016261-miR-150-5p-RBM48 and lncRNA XLOC_011677-miR-10b-5p-BEND3 were identified from the network. Conclusion: The current study revealed potential molecular biological regulation pathways and diagnostic biomarkers through the exosomal ceRNA regulatory network.", "claims": [{"id": "claim-method", "statement": "Mann–Whitney U or t-test were used to perform the comparisons. Receiver operating characteristic (ROC) curves with area under the curve (AUC) were used to determine the corresponding cutoff values, with pathological diagnosis as the “gold standard”.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36185995", "locator": "sections[12] \"Statistical Analysis\" paragraph 2", "quote": "Mann–Whitney U or t-test were used to perform the comparisons. Receiver operating characteristic (ROC) curves with area under the curve (AUC) were used to determine the corresponding cutoff values, with pathological diagnosis as the “gold standard”."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36185995", "locator": "sections[20] \"Data Availability Statement\" paragraph 2", "quote": "The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The current study revealed potential molecular biological regulation pathways and diagnostic biomarkers through the exosomal ceRNA regulatory network.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36185995", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "The current study revealed potential molecular biological regulation pathways and diagnostic biomarkers through the exosomal ceRNA regulatory network."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The current study revealed potential molecular biological regulation pathways and diagnostic biomarkers through the exosomal ceRNA regulatory network.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36185995"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Kyoto Encyclopedia of Genes and Genomes (KE", "eening criterion was fold change >1.5 in the microarray. Differentially expressed exosomal miRNAs we", "he target miRNAs of top 40 DE lncRNAs in the microarray expression profiles using TargetScan and miR", "cted mRNAs with the exosomal DE mRNAs in the microarray, and finally constructed 27 mRNAs targeted b"], "order_basis": "paper_text_order:548", "id": "assay:1"}, {"role": "analysis", "definition_id": "sequence_alignment", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["sequence_align", "alignment_identity"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 0.8, "evidence": ["read mapping"], "order_basis": "consumes_earlier_output", "id": "assay:2"}]}