Decoding competitive endogenous RNA regulatory network in postoperative cognitive dysfunction.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36203795", "kind": "science-record", "revision": 3, "content_hash": "sha256:9bd6298fd918f2830ca92182f6dadda0e1a22e027916bff85cf864a03b3db4ba", "created_at": "2026-11-01T18:15:31Z", "updated_at": "2026-11-01T21:29:06Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Decoding competitive endogenous RNA regulatory network in postoperative cognitive dysfunction.", "doi": "10.3389/fnins.2022.972918", "pmid": "36203795", "authors": ["Wei Wang", "Pengwei Huo", "Lei Zhang", "Gang Lv", "Zhongyuan Xia"], "journal": "Frontiers in neuroscience", "year": 2022, "abstract": "Postoperative cognitive dysfunction (POCD) is a common postoperative neurological complication in elderly patients. Circular RNAs (circRNAs) are abundant in the mammalian brain and can probably regulate cognitive function. However, the competitive endogenous RNA (ceRNA) regulatory network in POCD remains illiterate. Transcriptomic signatures in the hippocampus of POCD mice derived from the Gene Expression Omnibus (GEO) dataset GSE190880, GSE95070, and GSE115440 were used to identify the circRNA, miRNA, and mRNA expression profiles of POCD mice compared with controls, respectively. A set of differentially expressed RNAs, including 119 circRNAs, 33 miRNAs, and 49 mRNAs were identified. Transcript validation showed the enhanced expression of circ_0001634, circ_0001345, and circ_0001493. A ceRNA regulatory network composed of three circRNAs, three miRNAs, and six mRNAs was established. The hub mRNAs in the ceRNA network were further found to be involved in the hormone catabolic process and regulation of canonical Wnt signaling pathway, revealing their crucial role in POCD. Finally, three miRNAs and four mRNAs were verified by qRT-PCR. These results based on bioinformatics and PCR array suggest that circ_0001634/miR-490-5p/Rbm47, circ_0001634/miR-490-5p/Sostdc1, circ_0001634/miR-7001-5p/Sostdc1, circ_0001345/miR-7001-5p/Sostdc1, and circ_0001493/miR-7001-5p/Sostdc1 may be novel diagnostic biomarkers and therapeutic targets for POCD.", "claims": [{"id": "claim-method", "statement": "Differentially expressed (DE) analysis between POCD and control hippocampus samples were performed using GEO2R (Barrett et al., 2013), an online program based on R language that can analyze any GEO series to compare two groups of data.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36203795", "locator": "sections[4] \"Identification of the differentially expressed circular RNAs, microRNAs, and mRNAs\" paragraph 2", "quote": "Differentially expressed (DE) analysis between POCD and control hippocampus samples were performed using GEO2R (Barrett et al., 2013), an online program based on R language that can analyze any GEO series to compare two groups of data."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The original contributions presented in this study are included in the article/Supplementary material, further inquiries can be directed to the corresponding author/s.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36203795", "locator": "sections[21] \"Data availability statement\" paragraph 2", "quote": "The original contributions presented in this study are included in the article/Supplementary material, further inquiries can be directed to the corresponding author/s."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study identified 119 differentially expressed circRNAs, 33 miRNAs, and 49 mRNAs and proposed five circRNA-miRNA-mRNA axes as potential diagnostic biomarkers and therapeutic targets for POCD.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36203795", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "A set of differentially expressed RNAs, including 119 circRNAs, 33 miRNAs, and 49 mRNAs were identified."}, {"asset": "source:pmid:36203795", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "These results based on bioinformatics and PCR array suggest that circ_0001634/miR-490-5p/Rbm47, circ_0001634/miR-490-5p/Sostdc1, circ_0001634/miR-7001-5p/Sostdc1, circ_0001345/miR-7001-5p/Sostdc1, and circ_0001493/miR-7001-5p/Sostdc1 may be novel diagnostic biomarkers and therapeutic targets for POCD."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study identified 119 differentially expressed circRNAs, 33 miRNAs, and 49 mRNAs and proposed five circRNA-miRNA-mRNA axes as potential diagnostic biomarkers and therapeutic targets for POCD.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36203795"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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