Identification of CeRNA Regulatory Networks in Atrial Fibrillation Using Nanodelivery.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36212960", "kind": "science-record", "revision": 5, "content_hash": "sha256:cb2fed229faef171492326af2e621fd62161e0356bf8849add23b2bd35ea7e01", "created_at": "2026-11-01T18:15:33Z", "updated_at": "2026-11-01T22:50:18Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification of CeRNA Regulatory Networks in Atrial Fibrillation Using Nanodelivery.", "doi": "10.1155/2022/1046905", "pmid": "36212960", "authors": ["Ping Lin", "Lingqiang Meng", "Lei Lyu"], "journal": "Evidence-based complementary and alternative medicine : eCAM", "year": 2022, "abstract": "The initiation and maintenance of AF is a complex biological process that is the ultimate manifestation of many cardiovascular diseases. And the pathogenesis of atrial fibrillation (AF) is unclear. Therefore, this study aimed to find the potential competing endogenous RNAs (ceRNAs) network and molecular dysregulation mechanism associated with AF. GSE135445, GSE2240, and GSE68475 were obtained from the Gene Expression Omnibus (GEO). Differential analysis was utilized to identify the differentially expressed mRNAs, miRNAs, and lncRNAs between AF and sinus rhythms (SR). AF-associated mRNAs and nanomaterials were screened and their biological functions and KEGG signaling pathways were identified. Nanomaterials for targeted delivery are uniquely capable of localizing the delivery of therapeutics and diagnostics to diseased tissues. The target mRNAs and target lncRNAs of differentially expressed miRNAs were identified using TargetScan and LncBase databases. Finally, we constructed the ceRNAs network and its potential molecular regulatory mechanism. We obtained 643 AF-associated mRNAs. They were significantly involved in focal adhesion and the PI3K-Akt signaling pathway. Among the 16 differentially expressed miRNAs identified, 31 differentially expressed target mRNAs, as well as 5 differentially expressed target lncRNAs were identified. Among them, we obtained 2 ceRNAs networks (hsa-miR-125a-5p and hsa-let-7a-3p). The aberrant expression of network target genes in AF mainly activated the HIF-1 signaling pathway. We speculated that the interaction pairs of miR-125a-5p and let-7a-3p with target mRNAs and target lncRNAs may be involved in AF. Our findings have a positive influence on investigating the pathogenesis of AF and identifying potential therapeutic targets.", "claims": [{"id": "claim-method", "statement": "Difference analysis for mRNAs and lncRNAs between AF and SR in GSE135445 was performed using the edger R package [18]. The limma R package [19] was used for the difference analysis of mRNAs and miRNAs between AF and SR in GSE2240 and GSE68475.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36212960", "locator": "sections[4] \"2.2. Difference Analysis\" paragraph 2", "quote": "Difference analysis for mRNAs and lncRNAs between AF and SR in GSE135445 was performed using the edger R package [18]. The limma R package [19] was used for the difference analysis of mRNAs and miRNAs between AF and SR in GSE2240 and GSE68475."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36212960", "locator": "sections[15] \"Data Availability\" paragraph 2", "quote": "The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study identified 643 common differentially expressed mRNAs associated with AF by comparing GSE135445 with GSE2240.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36212960", "locator": "sections[9] \"3.1. Identification of AF-Associated mRNAs\" paragraph 2", "quote": "We obtained 7084 differentially expressed mRNAs in GSE2240, and by comparing them with those of GSE135445, we identified 643 common differentially expressed mRNAs (Figure 1(c))."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study identified 643 common differentially expressed mRNAs associated with AF by comparing GSE135445 with GSE2240.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36212960"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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