Leucine zipper protein 2 serves as a prognostic biomarker for prostate cancer correlating with immune infiltration and epigenetic regulation.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36217461", "kind": "science-record", "revision": 3, "content_hash": "sha256:849c29e020b607cf2fb897230f9a3ff186b566d16aec3ab088b8442e4a269505", "created_at": "2026-11-01T18:15:34Z", "updated_at": "2026-11-01T21:29:09Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Leucine zipper protein 2 serves as a prognostic biomarker for prostate cancer correlating with immune infiltration and epigenetic regulation.", "doi": "10.1016/j.heliyon.2022.e10750", "pmid": "36217461", "authors": ["Dechao Feng", "Weizhen Zhu", "Xu Shi", "Wuran Wei", "Ping Han", "Qiang Wei", "Lu Yang"], "journal": "Heliyon", "year": 2022, "abstract": "Background: We sought to determine whether leucine zipper protein 2 (LUZP2) could benefit men with prostate cancer (PCa) undergoing radical radiotherapy (RT) or prostatectomy (RP).\nMethods: Analysis was done on differentiating expression, clinical prognosis, co-expressed genes, immune infiltration, and epigenetic changes. All of our analyses were done using the R software (version 3.6.3) and the appropriate packages.\nResults: In terms of PCa, tumor samples expressed LUZP2 more than normal samples did. In the TCGA database and GSE116918, we found that LUZP2 was the only independent risk factor for PCa. The shared enriched pathways for patients undergoing RP or RT were cell-cell adhesion, regulation of filopodium assembly, and extracellular matrix containing collagen. With the exception of TNFRSF14, we discovered that LUZP2 was negatively correlated with 21 immune checkpoints in PCa patients receiving RT. We found a significant inverse relationship between LUZP2 expression and the tumor immune environment, which included B cells, CD4+ T cells, neutrophils, macrophages, dendritic cells, stromal score, immune score, and estimate score, in patients receiving RP or RT. Additionally, tumor purity was positively correlated with LUZP2. We found that the drug bortezomib may be susceptible to the LUZP2. DNA methylation was significantly associated with the mRNA expression of LUZP2 in PCa patients from the TCGA database, and LUZP2 methylation was positively correlated with immune cells. The proliferative activity of various PCa cells, which correlated to different stages of this disease, was also found to be significantly reduced by LUZP2 reduction, according to the results of our experimental work.\nConclusions: We proposed a relatively comprehensive understanding of the roles of LUZP2 on PCa from the fresh perspective of senescence.", "claims": [{"id": "claim-method", "statement": "Through univariate and multivariate COX regression analysis, we were able to identify the independent risk gene (LUZP2) for PCa patients undergoing RP or RT.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36217461", "locator": "sections[4] \"Data collection\" paragraph 2", "quote": "Through univariate and multivariate COX regression analysis, we were able to identify the independent risk gene (LUZP2) for PCa patients undergoing RP or RT."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data included in article/supp. material/referenced in article.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36217461", "locator": "sections[20] \"Data availability statement\" paragraph 2", "quote": "Data included in article/supp. material/referenced in article."}], "state": "complete"}, {"id": "claim-target-result", "statement": "LUZP2 was the only independent risk factor for prostate cancer in both the TCGA database and GSE116918.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36217461", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "In the TCGA database and GSE116918, we found that LUZP2 was the only independent risk factor for PCa."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "LUZP2 was the only independent risk factor for prostate cancer in both the TCGA database and GSE116918.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36217461"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE116918"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE116918"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:36217461", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1016/j.heliyon.2022.e10750", "sha256": 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