Oncogenic Role of HMGB1 as An Alarming in Robust Prediction of Immunotherapy Response in Colorectal Cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36230798", "kind": "science-record", "revision": 3, "content_hash": "sha256:ffdb054fd05ae79fdd530317c5ad02941c599287f616ec66e03c18505d622f6e", "created_at": "2026-11-01T18:15:36Z", "updated_at": "2026-11-01T21:29:11Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Oncogenic Role of HMGB1 as An Alarming in Robust Prediction of Immunotherapy Response in Colorectal Cancer.", "doi": "10.3390/cancers14194875", "pmid": "36230798", "authors": ["Huijiao Lu", "Mengyi Zhu", "Lin Qu", "Hongwei Shao", "Rongxin Zhang", "Yan Li"], "journal": "Cancers", "year": 2022, "abstract": "OBJECTIVE: To assess the correlation between HMGB1 expression and the patient prognosis in a multicancer context.\nMETHODS: The potential oncogenic role of HMGB1 was explored in forty tumors through the TCGA, GEO, and Oncomine datasets. We analyzed the clinical prognostic value and antitumor immunotherapy of HMGB1 in a multicancer context using GEO (GSE111636).\nRESULTS: High expression of HMGB1 is present in multicancer cases, and its low expression is closely associated with the prognostic survival of patients, in terms of both overall and disease-free survival in ACC and LUAD. Further investigation revealed that the high expression of gastric and lung cancer is closely associated with low risk and better prognosis of patients based on COX and Kaplan-Meier analysis of OS, FP and PPS. HMGB1 expression was found to be significantly correlated with cancer-associated fibroblast and CD8+ T cell infiltration in the TME. The analysis of GO functional annotation/KEGG pathways indicates that HMGB1 may regulate tumor immunity-related pathways, such as the tumor immunotherapy response in colorectal cancer. The function of four genes as hubs are confirmed by in vitro HMGB1 knockdown which led to inhibition of cell proliferation and metastasis in SW620 and SW480 cells.\nCONCLUSION: HMGB1 is a potential novel biomarker for improving clinical prognosis and antitumor immunotherapy efficacy. CDK1, HMGB2, SSRP1, and H2AFV may serve as key nodes for HMGB1 in colorectal cancer.", "claims": [{"id": "claim-method", "statement": "We explored survival prognosis of HMGB1 in different tumors. We logged into the site of GEPIA2 web server for OS and DFS data by setting high and low Cutoff value of 50%. Log-rank p-value was calculated and less than 0.05 was significant statistically.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36230798", "locator": "sections[4] \"2.2. Various Survival Prognosis Analysis\" paragraph 2", "quote": "We explored survival prognosis of HMGB1 in different tumors. We logged into the site of GEPIA2 web server for OS and DFS data by setting high and low Cutoff value of 50%. Log-rank p-value was calculated and less than 0.05 was significant statistically."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The authors certify that all the original data in this research could be obtained from public database. Other data used to support the findings of this study are included within the supplementary information files. All the raw data of this study are available from the first author or corresponding author upon request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36230798", "locator": "sections[31] \"notes\" paragraph 1", "quote": "The authors certify that all the original data in this research could be obtained from public database. Other data used to support the findings of this study are included within the supplementary information files. 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Through in vitro cell scratching,", "WB techniques, we also confirmed that HMGB1 knockdown led to inhibited proliferation and metastasi"], "order_basis": "paper_text_order:1243", "id": "assay:1"}, {"role": "data_generation", "definition_id": "methylation_array", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.85, "evidence": ["ERSORT-ABS, QUANTISEQ, XCELL, MCPCOUNTER and EPIC were utilized for immune infiltration estima"], "order_basis": "paper_text_order:3759", "id": "assay:2"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["of HMGB1. Through in vitro cell scratching, RT-qPCR, and WB techniques, we also confirmed that H", "HMGB1. Through in vitro cell scratching, RT-qPCR, and WB techniques, we also confirmed that H"], "order_basis": "paper_text_order:6167", "id": "assay:3"}, {"role": "data_generation", "definition_id": "ihc", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["1, CDK1, HMGB2, SSRP1, and H2AFV for COAD by immunohistochemistry (IHC) basedon the HPA database (Figure 7h).", "and H2AFV for COAD by immunohistochemistry (IHC) basedon the HPA database (Figure 7h)."], "order_basis": "paper_text_order:8975", "id": "assay:4"}, {"role": "analysis", "definition_id": "survival_analysis", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["logrank_test"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["We explored survival prognosis of HMGB1 in different tumors. 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