Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans.

{"sci_version": "1.0", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36232439", "kind": "science-record", "revision": 2, "content_hash": "sha256:67df855eb70485ebd4d7c499f1402208bf090f64d83aba7ff23ef428b471bce2", "created_at": "2026-11-01T18:15:36Z", "updated_at": "2026-11-01T19:00:33Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans.", "doi": "10.3390/ijms231911138", "pmid": "36232439", "authors": ["Joanna Gawlik", "Michal Koper", "Albert Bogdanowicz", "Piotr Weglenski", "Agnieszka Dzikowska"], "journal": "International journal of molecular sciences", "year": 2022, "abstract": "Kae1 is a subunit of the highly evolutionarily conserved KEOPS/EKC complex, which is involved in universal (t6A37) tRNA modification. Several reports have discussed the participation of this complex in transcription regulation in yeast and human cells, including our previous observations of KaeA, an Aspergillus nidulans homologue of Kae1p. The aim of this project was to confirm the role of KaeA in transcription, employing high-throughput transcriptomic (RNA-Seq and ChIP-Seq) and proteomic (LC-MS) analysis. We confirmed that KaeA is a subunit of the KEOPS complex in A. nidulans. An analysis of kaeA19 and kaeA25 mutants showed that, although the (t6A37) tRNA modification is unaffected in both mutants, they reveal significantly altered transcriptomes compared to the wild type. The finding that KaeA is localized in chromatin and identifying its protein partners allows us to postulate an additional nuclear function for the protein. Our data shed light on the universal bi-functional role of this factor and proves that the activity of this protein is not limited to tRNA modification in cytoplasm, but also affects the transcriptional activity of a number of nuclear genes. Data are available via the NCBI's GEO database under identifiers GSE206830 (RNA-Seq) and GSE206874 (ChIP-Seq), and via ProteomeXchange with identifier PXD034554 (proteomic).", "claims": [{"id": "claim-data-access", "statement": "The RNA-Seq transcriptomic data discussed in this publication have been deposited in the NCBI Gene Expression Omnibus database [62] and are accessible under GEO series accession number GSE206830 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE206830). The ChIP-Seq data discussed in this publication have been deposited in NCBI’s Gene Expression Omnibus [62] and are accessible under GEO series accession number GSE206874 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE206874). The mass spectrometry proteomics data discussed in this study are openly available from the ProteomeXchange Consortium [66] via the PRIDE [67] partner repository at Project DOI: 10.6019/PXD034554, with dataset identifier PXD034554.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36232439", "locator": "sections[26] \"notes\" paragraph 1", "quote": "The RNA-Seq transcriptomic data discussed in this publication have been deposited in the NCBI Gene Expression Omnibus database [62] and are accessible under GEO series accession number GSE206830 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE206830). The ChIP-Seq data discussed in this publication have been deposited in NCBI’s Gene Expression Omnibus [62] and are accessible under GEO series accession number GSE206874 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE206874). The mass spectrometry proteomics data discussed in this study are openly available from the ProteomeXchange Consortium [66] via the PRIDE [67] partner repository at Project DOI: 10.6019/PXD034554, with dataset identifier PXD034554."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Although the level of t6A37 modification is not affected in kaeA19 and kaeA25 mutants, RNA-Seq analysis confirmed that, compared to the wild type, the expression level of several hundred genes is changed in the mutants.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36232439", "locator": "sections[1] \"1. 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