A novel cuproptosis-related prognostic signature and potential value in HCC immunotherapy.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36250008", "kind": "science-record", "revision": 3, "content_hash": "sha256:d6ed57c3feeacce51ee31b7246f422b197e51fe126b28a00c5e15bf8dfd5eda3", "created_at": "2026-11-01T18:15:38Z", "updated_at": "2026-11-01T21:29:15Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "A novel cuproptosis-related prognostic signature and potential value in HCC immunotherapy.", "doi": "10.3389/fmolb.2022.1001788", "pmid": "36250008", "authors": ["Xiang-Xu Wang", "Li-Hong Wu", "Hongchen Ji", "Qing-Qing Liu", "Shi-Zhou Deng", "Qiong-Yi Dou", "Liping Ai", "Wei Pan", "Hong-Mei Zhang"], "journal": "Frontiers in molecular biosciences", "year": 2022, "abstract": "Background: Copper metabolism plays an important role in the tumor microenvironment, and cuproptosis is the last discovered programmed cell death process. However, the potential mechanism of cuproptosis in regulating the immune microenvironment of HCC remains unclear. Methods: A total of 716 HCC patients with complete mRNA expression and survival information were collected from three public HCC cohorts (TCGA-LIHC cohort, n = 370; GSE76427 cohort, n = 115; ICGC-LIRI cohort, n = 231). The unsupervised clustering analysis (NMF) was performed to identify three different cuproptosis-related subtypes. The univariate-Cox, lasso-Cox and multivariate-Cox regression analyses were performed to screen the cuproptosis related and construct the cuproptosis-related prognosis signature (Cu-PS). The immune cell infiltration was estimated by both CIBERSORT and MCPcounter algorithms. Results: This study identified three distinct cuproptosis-related metabolic patterns, which presented different pathway enrichment and immune cell infiltration. The Cu-PS, a 5-genes (C7, MAGEA6, HK2, CYP26B1 and EPO) signature, was significantly associated with TNM stage, tumor mutational burden (TMB), drugs sensitivity, and immunotherapies response. Conclusion: This study performed a multi-genetic analysis of cuproptosis-related genes and further explored the regulatory mechanism of cuproptosis in HCC. The Cu-PS might be a useful biomarker for predicting immunotherapy response and enhancing the diagnosis and treatment of HCC.", "claims": [{"id": "claim-method", "statement": "Based on the expression of these 16 cuproptosis-related genes, we performed an unsupervised clustering analysis (NMF) and identified three different cuproptosis-related subtypes.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36250008", "locator": "sections[4] \"Identification of HCC cuproptosis subtypes (Cu-clusters) based on NMF unsupervised clustering analysis\" paragraph 2", "quote": "Based on the expression of these 16 cuproptosis-related genes, we performed an unsupervised clustering analysis (NMF) and identified three different cuproptosis-related subtypes."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36250008", "locator": "sections[22] \"Data availability statement\" paragraph 2", "quote": "The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The Cu-PS, a 5-genes (C7, MAGEA6, HK2, CYP26B1 and EPO) signature, was significantly associated with TNM stage, tumor mutational burden (TMB), drugs sensitivity, and immunotherapies response.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36250008", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "The Cu-PS, a 5-genes (C7, MAGEA6, HK2, CYP26B1 and EPO) signature, was significantly associated with TNM stage, tumor mutational burden (TMB), drugs sensitivity, and immunotherapies response."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The Cu-PS, a 5-genes (C7, MAGEA6, HK2, CYP26B1 and EPO) signature, was significantly associated with TNM stage, tumor mutational burden (TMB), drugs sensitivity, and immunotherapies response.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36250008"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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