SFRP2 is a Novel Diagnostic Biomarker and Suppresses the Proliferation of Pituitary Adenoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36276274", "kind": "science-record", "revision": 5, "content_hash": "sha256:e1256f5f013c8920b084823488859e3d03b8b869cfc6477931b8e390aabb2890", "created_at": "2026-11-01T18:15:41Z", "updated_at": "2026-11-01T22:50:26Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "SFRP2 is a Novel Diagnostic Biomarker and Suppresses the Proliferation of Pituitary Adenoma.", "doi": "10.1155/2022/4272525", "pmid": "36276274", "authors": ["Rongxi Huang", "Danyan Chen", "Hongman Wang", "Binghan Zhang", "Yu Zhang", "Wei Ren"], "journal": "Journal of oncology", "year": 2022, "abstract": "Pituitary adenoma (PA) constitutes one of the most common intracranial tumors. The present study was designed to identify potential diagnostic markers for PA. We used gene expression profiles (GEO: GSE26966 and GEO: GSE63357 datasets) derived from human PA and nontumor samples that were made freely accessible by the gene expression omnibus (GEO) datasets. Differentially expressed genes (DEGs) were screened between 14 normal specimens and 34 PA specimens by the use of the limma package of the R. The diagnostic genes were determined using a LASSO regression model and SVM-RFE analysis. SFRP2 expression in PA cells was analyzed using RT-PCR, and the effect of SFRP2 dysregulation on PA cell proliferation was measured using CCK-8 analysis. In this study, 361 DEGs were identified: 309 genes were downregulated and 52 genes were upregulated. The results of KEGG assays revealed that the 361 DEGs were mainly enriched in the PI3K-Akt signaling pathway, MAPK signaling pathway, growth hormone synthesis, secretion and action, and AGE-RAGE signaling pathway in diabetic complications. Results from the LASSO regression model and the SVM-RFE analysis indicated that LOC101060391 and SFRP2 were diagnostic genes. In contrast to normal tissue, the expressions of LOC101060391 and SFRP2 were much lower in PA samples. According to the ROC assays, high LOC101060391 and SFRP2 expression had an AUC value >0.9 for PA. Upregulation of SFRP2 distinctly inhibited the proliferative capacity of PA cells, as shown by CCK-8 analysis. Furthermore, knockdown of SFRP2 had an influence on cell growth in both the AtT-20 and HP75 cell lines. Taken together, our findings indicate that LOC101060391 and SFRP2 have diagnostic potential for PA. Furthermore, SFRP2 may be an antioncogene and a therapeutic target for PA.", "claims": [{"id": "claim-method", "statement": "Differential expression analysis between 14 normal specimens and 34 tumor specimens was all performed with the help of the limma package of the R programming language (https://www.bioconductor.org/).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36276274", "locator": "sections[7] \"2.5. Data Processing and DEG Screening\" paragraph 2", "quote": "Differential expression analysis between 14 normal specimens and 34 tumor specimens was all performed with the help of the limma package of the R programming language (https://www.bioconductor.org/)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The original data are provided by the corresponding author upon request without any hesitation.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36276274", "locator": "sections[18] \"Data Availability\" paragraph 2", "quote": "The original data are provided by the corresponding author upon request without any hesitation."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Using limma for differential-expression analysis of two GEO datasets and LASSO regression plus SVM-RFE for biomarker selection, the study identified LOC101060391 and SFRP2 as diagnostic biomarkers for pituitary adenoma.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36276274", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "We used gene expression profiles (GEO: GSE26966 and GEO: GSE63357 datasets) derived from human PA and nontumor samples that were made freely accessible by the gene expression omnibus (GEO) datasets."}, {"asset": "source:pmid:36276274", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Differentially expressed genes (DEGs) were screened between 14 normal specimens and 34 PA specimens by the use of the limma package of the R."}, {"asset": "source:pmid:36276274", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Results from the LASSO regression model and the SVM-RFE analysis indicated that LOC101060391 and SFRP2 were diagnostic genes."}, {"asset": "source:pmid:36276274", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "According to the ROC assays, high LOC101060391 and SFRP2 expression had an AUC value >0.9 for PA."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Using limma for differential-expression analysis of two GEO datasets and LASSO regression plus SVM-RFE for biomarker selection, the study identified LOC101060391 and SFRP2 as diagnostic biomarkers for pituitary adenoma.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36276274"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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In this study, 361 DEGs were ident", "liferative capacity of PA cells, as shown by CCK-8 analysis. Furthermore, knockdown of SFRP2 ha"], "order_basis": "paper_text_order:777", "id": "assay:1"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ls, as shown by CCK-8 analysis. Furthermore, knockdown of SFRP2 had an influence on cell growth in", "Materials and Methods 2.1. Cell Culture and Transfection The HP75 and AtT-20 pituitary tumor cell lin", "density a full twenty-four hours before the transfection, and the plates were left to incubate overni", "e overnight. 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