The Construction and Analysis of a ceRNA Network Related to Salt-Sensitivity Hypertensives.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36277897", "kind": "science-record", "revision": 5, "content_hash": "sha256:de1cef32a504ecd03f7f31e2b1ebe48a1e8646926a37481a3877e0a9ff786727", "created_at": "2026-11-01T18:15:41Z", "updated_at": "2026-11-01T22:50:27Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "The Construction and Analysis of a ceRNA Network Related to Salt-Sensitivity Hypertensives.", "doi": "10.1155/2022/8258351", "pmid": "36277897", "authors": ["Xiu-Juan Liu", "Hong-Lin Yin", "Yan Li", "Hao Hao", "Yang Liu", "Quan-Lin Zhao"], "journal": "BioMed research international", "year": 2022, "abstract": "Background: Salt-sensitivity hypertensives (SSH) are an independent risk factor for cardiovascular disease. However, the mechanism of SSH is not clear. This study is aimed at constructing a competing endogenous RNA (ceRNA) network related to SSH.\nMethods: Data sets were collected from the Gene Expression Omnibus database (GEO) to extract data on salt sensitivity RNA of patients with or without hypertensives in GSE135111. Firstly, we analyzed differentially expressed genes (DEGs, log2FC ≥ 0.5 and P < 0.05) and differentially expressed lncRNAs (DELs, log2FC ≥1 and P<0.05) between SSH and salt-sensitive normotension (SSN). Then, the gene ontology (GO), KEGG pathway enrichment analysis, and PPI network construction of DEGs were performed, and the hub genes in the PPI network by cytoHubba (12 methods) were screened out. Finally, a ceRNA network was constructed based on lncRNA-miRNA-mRNA pairs and hub genes.\nResults: 163 DEGs and 65 DELs were screened out. The GO and KEGG pathway analyses of DEGs were mainly enriched in metabolism (e.g., insulin secretion and cellular response to glucagon stimulus and peptidyl-tyrosine dephosphorylation,) and plasma membrane signaling (e.g., cell adhesion and chemical synaptic transmission and integral component of membrane). Additionally, a ceRNA network, including 1 mRNA (EGLN3), 2 miRNAs (hsa-miR-17-5p and hsa-miR-20b-5p), and 1 lncRNA (C1orf143) was successfully constructed.\nConclusions: In conclusion, the proposed ceRNA network may help elucidate the regulatory mechanism by which lncRNAs function as ceRNAs and contribute to the pathogenesis of SSH. Importantly, candidate lncRNAs, miRNAs, and mRNAs can be further evaluated as a potential therapeutic targets for SSH.", "claims": [{"id": "claim-method", "statement": "In this study, the Limma package [6] was used to identify DEGs (|log2 FC| ≥ 0.5 and P value < 0.05), DELs (|log2 FC| ≥ 1 and P value < 0.05), and DECs (|log2 FC| ≥ 1 and P value <0.05).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36277897", "locator": "sections[4] \"2.2. Difference Analysis\" paragraph 2", "quote": "In this study, the Limma package [6] was used to identify DEGs (|log2 FC| ≥ 0.5 and P value < 0.05), DELs (|log2 FC| ≥ 1 and P value < 0.05), and DECs (|log2 FC| ≥ 1 and P value <0.05)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used during the current study are available from the corresponding authors upon reasonable request (GSE135111).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36277897", "locator": "sections[18] \"Data Availability\" paragraph 2", "quote": "The datasets used during the current study are available from the corresponding authors upon reasonable request (GSE135111)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "A ceRNA network including 1 mRNA (EGLN3), 2 miRNAs (hsa-miR-17-5p and hsa-miR-20b-5p), and 1 lncRNA (C1orf143) was successfully constructed.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36277897", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "Additionally, a ceRNA network, including 1 mRNA (EGLN3), 2 miRNAs (hsa-miR-17-5p and hsa-miR-20b-5p), and 1 lncRNA (C1orf143) was successfully constructed."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "A ceRNA network including 1 mRNA (EGLN3), 2 miRNAs (hsa-miR-17-5p and hsa-miR-20b-5p), and 1 lncRNA (C1orf143) was successfully constructed.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36277897"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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