TRPV3 inhibits colorectal cancer cell proliferation and migration by regulating the MAPK signaling pathway.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36388700", "kind": "science-record", "revision": 4, "content_hash": "sha256:9960ecf876345c57e6e93205994d0c5dca26a22f884f001c88c56c43ffffaeea", "created_at": "2026-11-01T18:15:49Z", "updated_at": "2026-11-01T22:08:05Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "TRPV3 inhibits colorectal cancer cell proliferation and migration by regulating the MAPK signaling pathway.", "doi": "10.21037/jgo-22-938", "pmid": "36388700", "authors": ["Wei Yu", "Jieping Huang", "Hui Yu", "Jingping Lin", "Fang Fan", "Ruixiang Xie", "Yongshi Shen", "Kangni Lin", "Yong Ye", "Jinsen Weng"], "journal": "Journal of gastrointestinal oncology", "year": 2022, "abstract": "Background: The aim of this study was to investigate the inhibiting effect of transient receptor potential vanilloid 3 (TRPV3) on the proliferation and migration of colorectal cancer (CRC) cells and to explore the underlying mechanism.\nMethods: A microarray dataset from the publicly available Gene Expression Omnibus (GEO) database was used to investigate the prognostic value of TRPV3 in CRC. In addition, 100 CRC tissue samples were collected at our center to further validate its prognostic value at the protein level. Cell proliferation ability was detected by Cell Counting Kit-8 (CCK-8) assay, and cell migration ability was detected by transwell assay. Gene set variation analysis (GSVA) was performed to identify the potential pathways regulated by TRPV3.\nResults: Based on the largest microarray dataset (GSE39582), low expression of TRPV3 was found to be significantly associated with poor prognosis in CRC patients, and this result was successfully validated at our cancer center. Functional experiments showed that knockdown of TRPV3 enhanced cell proliferation and migration, while enforced TRPV3 expression exhibited the opposite effect. GSEA based on public microarray data revealed that the mitogen-activated protein kinase (MAPK) signaling pathway was notably activated in patients with low expression of TRPV3. Further experiments in vivo confirmed that TRPV3 silencing promoted cell proliferation and migration by activating the MAPK signaling pathway.\nConclusions: Low expression of TRPV3, which stimulates cell proliferation and migration by provoking the MAPK signaling pathway, indicated poor prognosis in CRC patients.", "claims": [{"id": "claim-method", "statement": "Kaplan-Meier survival analysis was performed to compare the difference between survival curves.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36388700", "locator": "sections[12] \"Statistical analysis\" paragraph 2", "quote": "Kaplan-Meier survival analysis was performed to compare the difference between survival curves."}], "state": "complete"}, {"id": "claim-data-access", "statement": "To investigate the prognostic value of TRPV3 in CRC, we downloaded the largest microarray dataset (GSE39582) from the GEO database. This dataset consists of 557 CRC patients with transcriptome expression data and complete follow-up information.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36388700", "locator": "sections[14] \"Low expression of TRPV3 indicates poor prognosis in CRC patients\" paragraph 2", "quote": "To investigate the prognostic value of TRPV3 in CRC, we downloaded the largest microarray dataset (GSE39582) from the GEO database. This dataset consists of 557 CRC patients with transcriptome expression data and complete follow-up information."}], "state": "complete"}, {"id": "claim-target-result", "statement": "patients with low expression of TRPV3 exhibited significantly poorer overall survival than patients with high TRPV3 expression (P=0.045; Figure 1C).", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36388700", "locator": "sections[14] \"Low expression of TRPV3 indicates poor prognosis in CRC patients\" paragraph 2", "quote": "Based on the X-tile program (Figure 1A), patients were divided in low- and high-level groups (Figure 1B,1C), and patients with low expression of TRPV3 exhibited significantly poorer overall survival than patients with high TRPV3 expression (P=0.045; Figure 1C)."}, {"asset": "source:pmid:36388700", "locator": "sections[14] \"Low expression of TRPV3 indicates poor prognosis in CRC patients\" paragraph 4", "quote": "The results showed that TRPV3 was highly expressed in adjacent normal tissues (P<0.001; Figure 1D) and low expression of TRPV3 was significantly associated with poor overall survival (P=0.008; Figure 1E)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "patients with low expression of TRPV3 exhibited significantly poorer overall survival than patients with high TRPV3 expression (P=0.045; Figure 1C).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36388700"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE39582"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE39582"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:36388700", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.21037/jgo-22-938", "sha256": "sha256:3cf7213c8086dc6d40f31180176c86814ceeaae29bae92c48454b255188111f1", "bytes": 31080, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.21037/jgo-22-938", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC9660048", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE39582", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39582", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE39582", "version": null, "note": "To investigate the prognostic value of TRPV3 in CRC, we downloaded the largest microarray dataset (GSE39582) from the GEO database. 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Methods: A microarray dataset from the publicly available Gene Exp", "ated by TRPV3. Results: Based on the largest microarray dataset (GSE39582), low expression of TRPV3", "ed the opposite effect. GSEA based on public microarray data revealed that the mitogen-activated pro", "e of TRPV3 in CRC, we downloaded the largest microarray dataset (GSE39582) from the GEO database. Th"], "order_basis": "paper_text_order:310", "id": "assay:1"}, {"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["ability was detected by Cell Counting Kit-8 (CCK-8) assay, and cell migration ability was detec"], "order_basis": "paper_text_order:650", "id": "assay:2"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.85, "evidence": ["r center. Functional experiments showed that knockdown of TRPV3 enhanced cell proliferation and mig"], "order_basis": "paper_text_order:1091", "id": "assay:3"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.77, "evidence": ["low expression of TRPV3. Further experiments in vivo confirmed that TRPV3 silencing promoted cell"], "order_basis": "paper_text_order:1413", "id": "assay:4"}, {"role": "data_generation", "definition_id": "ihc", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["er plot is displayed in the right panel (C). Immunohistochemical staining of CRC tumor tissues and paired nor", "CRC cancer and paracancer tissues to perform immunohistochemistry staining analysis. Among the 100 patients, 4"], "order_basis": "paper_text_order:4952", "id": "assay:5"}, {"role": "analysis", "definition_id": "survival_analysis", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["survival_km"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Kaplan-Meier survival analysis was performed to compare the difference between survival curves."], "order_basis": "consumes_earlier_output", "id": "assay:6"}]}