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pmid:36389681
Differences of macrophages in the tumor microenvironment as an underlying key factor in glioma patients.
Yangyang Wang · Yan Liu · Chengkai Zhang · Chuanbao Zhang · Xiudong Guan · Wang Jia
Frontiers in immunology · 2022 doi:10.3389/fimmu.2022.1028937
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Experiment
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Data
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Analysis
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Execution
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Complete file
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Further studies are needed to investigate macrophages, which will be helpful to fully understand the role of it and early achieve clinical translation.\nMethods: A total of 1334 glioma cases were enrolled in this study from 3 databases. In our works, the single cell cohorts from GSE89567, GSE84465, and the Chinese Glioma Genome Atlas (CGGA) datasets were used to analyze the key genes of macrophage. The bulk sequencing data from the Cancer Genome Atlas (TCGA) and CGGA datasets were respectively divided into the training set and validation set to test prognostic value of the key genes from single cell analysis.\nResults: Quantitative and functional differences significantly emerge in macrophage clusters between LGG and GBM. Firstly, we used the Seurat R package to identify 281 genes differentially expressed genes in macrophage clusters between LGG and GBM. Furthermore, based on these genes, we developed a predictive risk model to predict prognosis and reflect the immune microenvironment in glioma. The risk score calculation formula was yielded as follows: Risk score = (0.11 × EXPMACC1) + (-0.31 × EXPOTUD1) + (-0.09 × EXPTCHH) + (0.26 × EXPADPRH) + (-0.40× EXPABCG2) + (0.21 × EXPPLBD1) + (0.12 × EXPANG) + (0.29 × EXPQPCT). The risk score was independently related to prognosis. Further, significant differences existed in immunological characteristics between the low- and high-risk score groups. What is more, mutation analysis found different genomic patterns associated with the risk score.\nConclusion: This study further confirms that the proportion of macrophage infiltration is not only significantly different, but the function of them is also different. The signature, identified from the differentially expressed macrophage-related genes impacts poor prognosis and short overall survival and may act as therapeutic targets in the future.", "claims": [{"id": "claim-method", "statement": "Kaplan-Meier (KM) was utilized to compare HR and LR patient survival via the Log-rank.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36389681", "locator": "sections[10] \"Statistical analysis\" paragraph 2", "quote": "Kaplan-Meier (KM) was utilized to compare HR and LR patient survival via the Log-rank."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data are available in a public, open access repository. 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This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE84465"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE84465"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:36389681", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.3389/fimmu.2022.1028937", "sha256": "sha256:26e7b2beff8efee0218ffa62cc53b15710990de39dba8e754d5358b883d25303", "bytes": 28609, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.3389/fimmu.2022.1028937", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC9659848", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE84465", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84465", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE84465", "version": null, "note": "Data are available in a public, open access repository. 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"GSM2244691", "GSM2244692", "GSM2244693", "GSM2244694", "GSM2244695", "GSM2244696", "GSM2244697", "GSM2244698", "GSM2244699", "GSM2244700", "GSM2244701", "GSM2244702", "GSM2244703", "GSM2244704", "GSM2244705", "GSM2244706", "GSM2244707", "GSM2244708", "GSM2244709", "GSM2244710", "GSM2244711", "GSM2244712", "GSM2244713", "GSM2244714", "GSM2244715", "GSM2244716", "GSM2244717", "GSM2244718", "GSM2244719", "GSM2244720", "GSM2244721", "GSM2244722", "GSM2244723", "GSM2244724", "GSM2244725", "GSM2244726", "GSM2244727", "GSM2244728", "GSM2244729", "GSM2244730", "GSM2244731", "GSM2244732", "GSM2244733", "GSM2244734", "GSM2244735", "GSM2244736", "GSM2244737", "GSM2244738", "GSM2244739", "GSM2244740", "GSM2244741", "GSM2244742", "GSM2244743", "GSM2244744", "GSM2244745", "GSM2244746", "GSM2244747", "GSM2244748", "GSM2244749", "GSM2244750", "GSM2244751", "GSM2244752", "GSM2244753", "GSM2244754", "GSM2244755", "GSM2244756", "GSM2244757", "GSM2244758", "GSM2244759", "GSM2244760", "GSM2244761", 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"GSM2245685", "GSM2245686", "GSM2245687", "GSM2245688", "GSM2245689", "GSM2245690", "GSM2245691", "GSM2245692", "GSM2245693", "GSM2245694", "GSM2245695", "GSM2245696", "GSM2245697", "GSM2245698", "GSM2245699", "GSM2245700", "GSM2245701", "GSM2245702", "GSM2245703", "GSM2245704", "GSM2245705", "GSM2245706", "GSM2245707", "GSM2245708", "GSM2245709", "GSM2245710", "GSM2245711", "GSM2245712", "GSM2245713", "GSM2245714", "GSM2245715", "GSM2245716", "GSM2245717", "GSM2245718", "GSM2245719", "GSM2245720", "GSM2245721", "GSM2245722", "GSM2245723", "GSM2245724", "GSM2245725", "GSM2245726", "GSM2245727", "GSM2245728", "GSM2245729", "GSM2245730", "GSM2245731", "GSM2245732", "GSM2245733", "GSM2245734", "GSM2245735", "GSM2245736", "GSM2245737", "GSM2245738", "GSM2245739", "GSM2245740", "GSM2245741", "GSM2245742", "GSM2245743", "GSM2245744", "GSM2245745", "GSM2245746", "GSM2245747", "GSM2245748", "GSM2245749", "GSM2245750", "GSM2245751", "GSM2245752", "GSM2245753", "GSM2245754", "GSM2245755", "GSM2245756", "GSM2245757", "GSM2245758", "GSM2245759", "GSM2245760", "GSM2245761", "GSM2245762", "GSM2245763", "GSM2245764", "GSM2245765", "GSM2245766", "GSM2245767", "GSM2245768", "GSM2245769", "GSM2245770", "GSM2245771", "GSM2245772", "GSM2245773", "GSM2245774", "GSM2245775", "GSM2245776", "GSM2245777", "GSM2245778", "GSM2245779", "GSM2245780", "GSM2245781", "GSM2245782", "GSM2245783", "GSM2245784", "GSM2245785", "GSM2245786", "GSM2245787", "GSM2245788", "GSM2245789", "GSM2245790", "GSM2245791", "GSM2245792", "GSM2245793", "GSM2245794", "GSM2245795", "GSM2245796", "GSM2245797", "GSM2245798", "GSM2245799", "GSM2245800", "GSM2245801", "GSM2245802", "GSM2245803", "GSM2245804", "GSM2245805", "GSM2245806", "GSM2245807", "GSM2245808", "GSM2245809", "GSM2245810", "GSM2245811", "GSM2245812", "GSM2245813", "GSM2245814", "GSM2245815", "GSM2245816", "GSM2245817", "GSM2245818", "GSM2245819", "GSM2245820", "GSM2245821", "GSM2245822", "GSM2245823", "GSM2245824", "GSM2245825", "GSM2245826", "GSM2245827", "GSM2245828", "GSM2245829", "GSM2245830", "GSM2245831", "GSM2245832", "GSM2245833", "GSM2245834", "GSM2245835", "GSM2245836", "GSM2245837", "GSM2245838", "GSM2245839", "GSM2245840", "GSM2245841", "GSM2245842", "GSM2245843", "GSM2245844", "GSM2245845", "GSM2245846", "GSM2245847", "GSM2245848", "GSM2245849", "GSM2245850", "GSM2245851", "GSM2245852", "GSM2245853", "GSM2245854", "GSM2245855", "GSM2245856", "GSM2245857", "GSM2245858", "GSM2245859", "GSM2245860", "GSM2245861", "GSM2245862", "GSM2245863", "GSM2245864", "GSM2245865", "GSM2245866", "GSM2245867", "GSM2245868", "GSM2245869", "GSM2245870", "GSM2245871", "GSM2245872", "GSM2245873", "GSM2245874", "GSM2245875", "GSM2245876", "GSM2245877", "GSM2245878", "GSM2245879", "GSM2245880", "GSM2245881", "GSM2245882", "GSM2245883", "GSM2245884", "GSM2245885", "GSM2245886", "GSM2245887", "GSM2245888", "GSM2245889", "GSM2245890", "GSM2245891", "GSM2245892", "GSM2245893", "GSM2245894", "GSM2245895", "GSM2245896", "GSM2245897", 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"sample_count": 3589, "group_sizes": null, "group_sizes_basis": null, "platform": "18573", "taxon": "Homo sapiens", "title": "Single-Cell RNAseq analysis of diffuse neoplastic infiltrating cells at the migrating front of human glioblastoma", "resolution_checked_at": "2026-11-01T15:39:55Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE89567", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE89567", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE89567", "version": null, "note": "Data are available in a public, open access repository. The datasets analyzed during the current study are available in the Gene Expression Omnibus (GSE89567: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE89567; GSE84465: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84465) and The Cancer Genome Atlas (TCGA) (https://cancergenome.nih.gov/) and the Chinese Glioma Genome Atlas (http://www.cgga.org.cn/).", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "Data are available in a public, open access repository. The datasets analyzed during the current study are available in the Gene Expression Omnibus (GSE89567: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE89567; GSE84465: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84465) and The Cancer Genome Atlas (TCGA) (https://cancergenome.nih.gov/) and the Chinese Glioma Genome Atlas (http://www.cgga.org.cn/).", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM2377571", "GSM2377572", "GSM2377573", "GSM2377574", "GSM2377575", "GSM2377576", "GSM2377577", "GSM2377578", "GSM2377579", "GSM2377580", "GSM2377581", "GSM2377582", "GSM2377583", "GSM2377584", "GSM2377585", "GSM2377586", "GSM2377587", "GSM2377588", "GSM2377589", "GSM2377590", "GSM2377591", "GSM2377592", "GSM2377593", "GSM2377594", "GSM2377595", "GSM2377596", "GSM2377597", "GSM2377598", "GSM2377599", "GSM2377600", "GSM2377601", "GSM2377602", "GSM2377603", "GSM2377604", "GSM2377605", "GSM2377606", "GSM2377607", "GSM2377608", "GSM2377609", 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"GSM2380592", "GSM2380593", "GSM2380594", "GSM2380595", "GSM2380596", "GSM2380597", "GSM2380598", "GSM2380599", "GSM2380600", "GSM2380601", "GSM2380602", "GSM2380603", "GSM2380604", "GSM2380605", "GSM2380606", "GSM2380607", "GSM2380608", "GSM2380609", "GSM2380610", "GSM2380611", "GSM2380612", "GSM2380613", "GSM2380614", "GSM2380615", "GSM2380616", "GSM2380617", "GSM2380618", "GSM2380619", "GSM2380620", "GSM2380621", "GSM2380622", "GSM2380623", "GSM2380624", "GSM2380625", "GSM2380626", "GSM2380627", "GSM2380628", "GSM2380629", "GSM2380630", "GSM2380631", "GSM2380632", "GSM2380633", "GSM2380634", "GSM2380635", "GSM2380636", "GSM2380637", "GSM2380638", "GSM2380639", "GSM2380640", "GSM2380641", "GSM2380642", "GSM2380643", "GSM2380644", "GSM2380645", "GSM2380646", "GSM2380647", "GSM2380648", "GSM2380649", "GSM2380650", "GSM2380651", "GSM2380652", "GSM2380653", "GSM2380654", "GSM2380655", "GSM2380656", "GSM2380657", "GSM2380658", "GSM2380659", "GSM2380660", "GSM2380661", "GSM2380662", 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"GSM2380947", "GSM2380948", "GSM2380949", "GSM2380950", "GSM2380951", "GSM2380952", "GSM2380953", "GSM2380954", "GSM2380955", "GSM2380956", "GSM2380957", "GSM2380958", "GSM2380959", "GSM2380960", "GSM2380961", "GSM2380962", "GSM2380963", "GSM2380964", "GSM2380965", "GSM2380966", "GSM2380967", "GSM2380968", "GSM2380969", "GSM2380970", "GSM2380971", "GSM2380972", "GSM2380973", "GSM2380974", "GSM2380975", "GSM2380976", "GSM2380977", "GSM2380978", "GSM2380979", "GSM2380980", "GSM2380981", "GSM2380982", "GSM2380983", "GSM2380984", "GSM2380985", "GSM2380986", "GSM2380987", "GSM2380988", "GSM2380989", "GSM2380990", "GSM2380991", "GSM2380992", "GSM2380993", "GSM2380994", "GSM2380995", "GSM2380996", "GSM2380997", "GSM2380998", "GSM2380999", "GSM2381000", "GSM2381001", "GSM2381002", "GSM2381003", "GSM2381004", "GSM2381005", "GSM2381006", "GSM2381007", "GSM2381008", "GSM2381009", "GSM2381010", "GSM2381011", "GSM2381012", "GSM2381013", "GSM2381014", "GSM2381015", "GSM2381016", "GSM2381017", "GSM2381018", "GSM2381019", "GSM2381020", "GSM2381021", "GSM2381022", "GSM2381023", "GSM2381024", "GSM2381025", "GSM2381026", "GSM2381027", "GSM2381028", "GSM2381029", "GSM2381030", "GSM2381031", "GSM2381032", "GSM2381033", "GSM2381034", "GSM2381035", "GSM2381036", "GSM2381037", "GSM2381038", "GSM2381039", "GSM2381040", "GSM2381041", "GSM2381042", "GSM2381043", "GSM2381044", "GSM2381045", "GSM2381046", "GSM2381047", "GSM2381048", "GSM2381049", "GSM2381050", "GSM2381051", "GSM2381052", "GSM2381053", "GSM2381054", "GSM2381055", "GSM2381056", "GSM2381057", "GSM2381058", "GSM2381059", "GSM2381060", "GSM2381061", "GSM2381062", "GSM2381063", "GSM2381064", "GSM2381065", "GSM2381066", "GSM2381067", "GSM2381068", "GSM2381069", "GSM2381070", "GSM2381071", "GSM2381072", "GSM2381073", "GSM2381074", "GSM2381075", "GSM2381076", "GSM2381077", "GSM2381078", "GSM2381079", "GSM2381080", "GSM2381081", "GSM2381082", "GSM2381083", "GSM2381084", "GSM2381085", "GSM2381086", "GSM2381087", "GSM2381088", 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"GSM2381160", "GSM2381161", "GSM2381162", "GSM2381163", "GSM2381164", "GSM2381165", "GSM2381166", "GSM2381167", "GSM2381168", "GSM2381169", "GSM2381170", "GSM2381171", "GSM2381172", "GSM2381173", "GSM2381174", "GSM2381175", "GSM2381176", "GSM2381177", "GSM2381178", "GSM2381179", "GSM2381180", "GSM2381181", "GSM2381182", "GSM2381183", "GSM2381184", "GSM2381185", "GSM2381186", "GSM2381187", "GSM2381188", "GSM2381189", "GSM2381190", "GSM2381191", "GSM2381192", "GSM2381193", "GSM2381194", "GSM2381195", "GSM2381196", "GSM2381197", "GSM2381198", "GSM2381199", "GSM2381200", "GSM2381201", "GSM2381202", "GSM2381203", "GSM2381204", "GSM2381205", "GSM2381206", "GSM2381207", "GSM2381208", "GSM2381209", "GSM2381210", "GSM2381211", "GSM2381212", "GSM2381213", "GSM2381214", "GSM2381215", "GSM2381216", "GSM2381217", "GSM2381218", "GSM2381219", "GSM2381220", "GSM2381221", "GSM2381222", "GSM2381223", "GSM2381224", "GSM2381225", "GSM2381226", "GSM2381227", "GSM2381228", "GSM2381229", "GSM2381230", "GSM2381231", "GSM2381232", "GSM2381233", "GSM2381234", "GSM2381235", "GSM2381236", "GSM2381237", "GSM2381238", "GSM2381239", "GSM2381240", "GSM2381241", "GSM2381242", "GSM2381243", "GSM2381244", "GSM2381245", "GSM2381246", "GSM2381247", "GSM2381248", "GSM2381249", "GSM2381250", "GSM2381251", "GSM2381252", "GSM2381253", "GSM2381254", "GSM2381255", "GSM2381256", "GSM2381257", "GSM2381258", "GSM2381259", "GSM2381260", "GSM2381261", "GSM2381262", "GSM2381263", "GSM2381264", "GSM2381265", "GSM2381266", "GSM2381267", "GSM2381268", "GSM2381269", "GSM2381270", "GSM2381271", "GSM2381272", "GSM2381273", "GSM2381274", "GSM2381275", "GSM2381276", "GSM2381277", "GSM2381278", "GSM2381279", "GSM2381280", "GSM2381281", "GSM2381282", "GSM2381283", "GSM2381284", "GSM2381285", "GSM2381286", "GSM2381287", "GSM2381288", "GSM2381289", "GSM2381290", "GSM2381291", "GSM2381292", "GSM2381293", "GSM2381294", "GSM2381295", "GSM2381296", "GSM2381297", "GSM2381298", "GSM2381299", "GSM2381300", "GSM2381301", "GSM2381302", "GSM2381303", "GSM2381304", "GSM2381305", "GSM2381306", "GSM2381307", "GSM2381308", "GSM2381309", "GSM2381310", "GSM2381311", "GSM2381312", "GSM2381313", "GSM2381314", "GSM2381315", "GSM2381316", "GSM2381317", "GSM2381318", "GSM2381319", "GSM2381320", "GSM2381321", "GSM2381322", "GSM2381323", "GSM2381324", "GSM2381325", "GSM2381326", "GSM2381327", "GSM2381328", "GSM2381329", "GSM2381330", "GSM2381331", "GSM2381332", "GSM2381333", "GSM2381334", "GSM2381335", "GSM2381336", "GSM2381337", "GSM2381338", "GSM2381339", "GSM2381340", "GSM2381341", "GSM2381342", "GSM2381343", "GSM2381344", "GSM2381345", "GSM2381346", "GSM2381347", "GSM2381348", "GSM2381349", "GSM2381350", "GSM2381351", "GSM2381352", "GSM2381353", "GSM2381354", "GSM2381355", "GSM2381356", "GSM2381357", "GSM2381358", "GSM2381359", "GSM2381360", "GSM2381361", "GSM2381362", "GSM2381363", "GSM2381364", "GSM2381365", "GSM2381366", "GSM2381367", "GSM2381368", "GSM2381369", "GSM2381370", "GSM2381371", "GSM2381372", "GSM2381373", "GSM2381374", "GSM2381375", "GSM2381376", "GSM2381377", "GSM2381378", "GSM2381379", "GSM2381380", "GSM2381381", "GSM2381382", "GSM2381383", "GSM2381384", "GSM2381385", "GSM2381386", "GSM2381387", "GSM2381388", "GSM2381389", "GSM2381390", "GSM2381391", "GSM2381392", "GSM2381393", "GSM2381394", "GSM2381395", "GSM2381396", "GSM2381397", "GSM2381398", "GSM2381399", "GSM2381400", "GSM2381401", "GSM2381402", "GSM2381403", "GSM2381404", "GSM2381405", "GSM2381406", "GSM2381407", "GSM2381408", "GSM2381409", "GSM2381410", "GSM2381411", "GSM2381412", "GSM2381413", "GSM2381414", "GSM2381415", "GSM2381416", "GSM2381417", "GSM2381418", "GSM2381419", "GSM2381420", "GSM2381421", "GSM2381422", "GSM2381423", "GSM2381424", "GSM2381425", "GSM2381426", "GSM2381427", "GSM2381428", "GSM2381429", "GSM2381430", "GSM2381431", "GSM2381432", "GSM2381433", "GSM2381434", "GSM2381435", "GSM2381436", "GSM2381437", "GSM2381438", "GSM2381439", "GSM2381440", "GSM2381441", "GSM2381442", "GSM2381443", "GSM2381444", "GSM2381445", "GSM2381446", "GSM2381447", "GSM2381448", "GSM2381449", "GSM2381450", "GSM2381451", "GSM2381452", "GSM2381453", "GSM2381454", "GSM2381455", "GSM2381456", "GSM2381457", "GSM2381458", "GSM2381459", "GSM2381460", "GSM2381461", "GSM2381462", "GSM2381463", "GSM2381464", "GSM2381465", "GSM2381466", "GSM2381467", "GSM2381468", "GSM2381469", "GSM2381470", "GSM2381471", "GSM2381472", "GSM2381473", "GSM2381474", "GSM2381475", "GSM2381476", "GSM2381477", "GSM2381478", "GSM2381479", "GSM2381480", "GSM2381481", "GSM2381482", "GSM2381483", "GSM2381484", "GSM2381485", "GSM2381486", "GSM2381487", "GSM2381488", "GSM2381489", "GSM2381490", "GSM2381491", "GSM2381492", "GSM2381493", "GSM2381494", "GSM2381495", "GSM2381496", "GSM2381497", "GSM2381498", "GSM2381499", "GSM2381500", "GSM2381501", "GSM2381502", "GSM2381503", "GSM2381504", "GSM2381505", "GSM2381506", "GSM2381507", "GSM2381508", "GSM2381509", "GSM2381510", "GSM2381511", "GSM2381512", "GSM2381513", "GSM2381514", "GSM2381515", "GSM2381516", "GSM2381517", "GSM2381518", "GSM2381519", "GSM2381520", "GSM2381521", "GSM2381522", "GSM2381523", "GSM2381524", "GSM2381525", "GSM2381526", "GSM2381527", "GSM2381528", "GSM2381529", "GSM2381530", "GSM2381531", "GSM2381532", "GSM2381533", "GSM2381534", "GSM2381535", "GSM2381536", "GSM2381537", "GSM2381538", "GSM2381539", "GSM2381540", "GSM2381541", "GSM2381542", "GSM2381543", "GSM2381544", "GSM2381545", "GSM2381546", "GSM2381547", "GSM2381548", "GSM2381549", "GSM2381550", "GSM2381551", "GSM2381552", "GSM2381553", "GSM2381554", "GSM2381555", "GSM2381556", "GSM2381557", "GSM2381558", "GSM2381559", "GSM2381560", "GSM2381561", "GSM2381562", "GSM2381563", "GSM2381564", "GSM2381565", "GSM2381566", "GSM2381567", "GSM2381568", "GSM2381569", "GSM2381570", "GSM2381571", "GSM2381572", "GSM2381573", "GSM2381574", "GSM2381575", "GSM2381576", "GSM2381577", "GSM2381578", "GSM2381579", "GSM2381580", "GSM2381581", "GSM2381582", "GSM2381583", "GSM2381584", "GSM2381585", "GSM2381586", "GSM2381587", "GSM2381588", "GSM2381589", "GSM2381590", "GSM2381591", "GSM2381592", "GSM2381593", "GSM2381594", "GSM2381595", "GSM2381596", "GSM2381597", "GSM2381598", "GSM2381599", "GSM2381600", "GSM2381601", "GSM2381602", "GSM2381603", "GSM2381604", "GSM2381605", "GSM2381606", "GSM2381607", "GSM2381608", "GSM2381609", "GSM2381610", "GSM2381611", "GSM2381612", "GSM2381613", "GSM2381614", "GSM2381615", "GSM2381616", "GSM2381617", "GSM2381618", "GSM2381619", "GSM2381620", "GSM2381621", "GSM2381622", "GSM2381623", "GSM2381624", "GSM2381625", "GSM2381626", "GSM2381627", "GSM2381628", "GSM2381629", "GSM2381630", "GSM2381631", "GSM2381632", "GSM2381633", "GSM2381634", "GSM2381635", "GSM2381636", "GSM2381637", "GSM2381638", "GSM2381639", "GSM2381640", "GSM2381641", "GSM2381642", "GSM2381643", "GSM2381644", "GSM2381645", "GSM2381646", "GSM2381647", "GSM2381648", "GSM2381649", "GSM2381650", "GSM2381651", "GSM2381652", "GSM2381653", "GSM2381654", "GSM2381655", "GSM2381656", "GSM2381657", "GSM2381658", "GSM2381659", "GSM2381660", "GSM2381661", "GSM2381662", "GSM2381663", "GSM2381664", "GSM2381665", "GSM2381666", "GSM2381667", "GSM2381668", "GSM2381669", "GSM2381670", "GSM2381671", "GSM2381672", "GSM2381673", "GSM2381674", "GSM2381675", "GSM2381676", "GSM2381677", "GSM2381678", "GSM2381679", "GSM2381680", "GSM2381681", "GSM2381682", "GSM2381683", "GSM2381684", "GSM2381685", "GSM2381686", "GSM2381687", "GSM2381688", "GSM2381689", "GSM2381690", "GSM2381691", "GSM2381692", "GSM2381693", "GSM2381694", "GSM2381695", "GSM2381696", "GSM2381697", "GSM2381698", "GSM2381699", "GSM2381700", "GSM2381701", "GSM2381702", "GSM2381703", "GSM2381704", "GSM2381705", "GSM2381706", "GSM2381707", "GSM2381708", "GSM2381709", "GSM2381710", "GSM2381711", "GSM2381712", "GSM2381713", "GSM2381714", "GSM2381715", "GSM2381716", "GSM2381717", "GSM2381718", "GSM2381719", "GSM2381720", "GSM2381721", "GSM2381722", "GSM2381723", "GSM2381724", "GSM2381725", "GSM2381726", "GSM2381727", "GSM2381728", "GSM2381729", "GSM2381730", "GSM2381731", "GSM2381732", "GSM2381733", "GSM2381734", "GSM2381735", "GSM2381736", "GSM2381737", "GSM2381738", "GSM2381739", "GSM2381740", "GSM2381741", "GSM2381742", "GSM2381743", "GSM2381744", "GSM2381745", "GSM2381746", "GSM2381747", "GSM2381748", "GSM2381749", "GSM2381750", "GSM2381751", "GSM2381752", "GSM2381753", "GSM2381754", "GSM2381755", "GSM2381756", "GSM2381757", "GSM2381758", "GSM2381759", "GSM2381760", "GSM2381761", "GSM2381762", "GSM2381763", "GSM2381764", "GSM2381765", "GSM2381766", "GSM2381767", "GSM2381768", "GSM2381769", "GSM2381770", "GSM2381771", "GSM2381772", "GSM2381773", "GSM2381774", "GSM2381775", "GSM2381776", "GSM2381777", "GSM2381778", "GSM2381779", "GSM2381780", "GSM2381781", "GSM2381782", "GSM2381783", "GSM2381784", "GSM2381785", "GSM2381786", "GSM2381787", "GSM2381788", "GSM2381789", "GSM2381790", "GSM2381791", "GSM2381792", "GSM2381793", "GSM2381794", "GSM2381795", "GSM2381796", "GSM2381797", "GSM2381798", "GSM2381799", "GSM2381800", "GSM2381801", "GSM2381802", "GSM2381803", "GSM2381804", "GSM2381805", "GSM2381806", "GSM2381807", "GSM2381808", "GSM2381809", "GSM2381810", "GSM2381811", "GSM2381812", "GSM2381813", "GSM2381814", "GSM2381815", "GSM2381816", "GSM2381817", "GSM2381818", "GSM2381819", "GSM2381820", "GSM2381821", "GSM2381822", "GSM2381823", "GSM2381824", "GSM2381825", "GSM2381826", "GSM2381827", "GSM2381828", "GSM2381829", "GSM2381830", "GSM2381831", "GSM2381832", "GSM2381833", "GSM2381834", "GSM2381835", "GSM2381836", "GSM2381837", "GSM2381838", "GSM2381839", "GSM2381840", "GSM2381841", "GSM2381842", "GSM2381843", "GSM2381844", "GSM2381845", "GSM2381846", "GSM2381847", "GSM2381848", "GSM2381849", "GSM2381850", "GSM2381851", "GSM2381852", "GSM2381853", "GSM2381854", "GSM2381855", "GSM2381856", "GSM2381857", "GSM2381858", "GSM2381859", "GSM2381860", "GSM2381861", "GSM2381862", "GSM2381863", "GSM2381864", "GSM2381865", "GSM2381866", "GSM2381867", "GSM2381868", "GSM2381869", "GSM2381870", "GSM2381871", "GSM2381872", "GSM2381873", "GSM2381874", "GSM2381875", "GSM2381876", "GSM2381877", "GSM2381878", "GSM2381879", "GSM2381880", "GSM2381881", "GSM2381882", "GSM2381883", "GSM2381884", "GSM2381885", "GSM2381886", "GSM2381887", "GSM2381888", "GSM2381889", "GSM2381890", "GSM2381891", "GSM2381892", "GSM2381893", "GSM2381894", "GSM2381895", "GSM2381896", "GSM2381897", "GSM2381898", "GSM2381899", "GSM2381900", "GSM2381901", "GSM2381902", "GSM2381903", "GSM2381904", "GSM2381905", "GSM2381906", "GSM2381907", "GSM2381908", "GSM2381909", "GSM2381910", "GSM2381911", "GSM2381912", "GSM2381913", "GSM2381914", "GSM2381915", "GSM2381916", "GSM2381917", "GSM2381918", "GSM2381919", "GSM2381920", "GSM2381921", "GSM2381922", "GSM2381923", "GSM2381924", "GSM2381925", "GSM2381926", "GSM2381927", "GSM2381928", "GSM2381929", "GSM2381930", "GSM2381931", "GSM2381932", "GSM2381933", "GSM2381934", "GSM2381935", "GSM2381936", "GSM2381937", "GSM2381938", "GSM2381939", "GSM2381940", "GSM2381941", "GSM2381942", "GSM2381943", "GSM2381944", "GSM2381945", "GSM2381946", "GSM2381947", "GSM2381948", "GSM2381949", "GSM2381950", "GSM2381951", "GSM2381952", "GSM2381953", "GSM2381954", "GSM2381955", "GSM2381956", "GSM2381957", "GSM2381958", "GSM2381959", "GSM2381960", "GSM2381961", "GSM2381962", "GSM2381963", "GSM2381964", "GSM2381965", "GSM2381966", "GSM2381967", "GSM2381968", "GSM2381969", "GSM2381970", "GSM2381971", "GSM2381972", "GSM2381973", "GSM2381974", "GSM2381975", "GSM2381976", "GSM2381977", "GSM2381978", "GSM2381979", "GSM2381980", "GSM2381981", "GSM2381982", "GSM2381983", "GSM2381984", "GSM2381985", "GSM2381986", "GSM2381987", "GSM2381988", "GSM2381989", "GSM2381990", "GSM2381991", "GSM2381992", "GSM2381993", "GSM2381994", "GSM2381995", "GSM2381996", "GSM2381997", "GSM2381998", "GSM2381999", "GSM2382000", "GSM2382001", "GSM2382002", "GSM2382003", "GSM2382004", "GSM2382005", "GSM2382006", "GSM2382007", "GSM2382008", "GSM2382009", "GSM2382010", "GSM2382011", "GSM2382012", "GSM2382013", "GSM2382014", "GSM2382015", "GSM2382016", "GSM2382017", "GSM2382018", "GSM2382019", "GSM2382020", "GSM2382021", "GSM2382022", "GSM2382023", "GSM2382024", "GSM2382025", "GSM2382026", "GSM2382027", "GSM2382028", "GSM2382029", "GSM2382030", "GSM2382031", "GSM2382032", "GSM2382033", "GSM2382034", "GSM2382035", "GSM2382036", "GSM2382037", "GSM2382038", "GSM2382039", "GSM2382040", "GSM2382041", "GSM2382042", "GSM2382043", "GSM2382044", "GSM2382045", "GSM2382046", "GSM2382047", "GSM2382048", "GSM2382049", "GSM2382050", "GSM2382051", "GSM2382052", "GSM2382053", "GSM2382054", "GSM2382055", "GSM2382056", "GSM2382057", "GSM2382058", "GSM2382059", "GSM2382060", "GSM2382061", "GSM2382062", "GSM2382063", "GSM2382064", "GSM2382065", "GSM2382066", "GSM2382067", "GSM2382068", "GSM2382069", "GSM2382070", "GSM2382071", "GSM2382072", "GSM2382073", "GSM2382074", "GSM2382075", "GSM2382076", "GSM2382077", "GSM2382078", "GSM2382079", "GSM2382080", "GSM2382081", "GSM2382082", "GSM2382083", "GSM2382084", "GSM2382085", "GSM2382086", "GSM2382087", "GSM2382088", "GSM2382089", "GSM2382090", "GSM2382091", "GSM2382092", "GSM2382093", "GSM2382094", "GSM2382095", "GSM2382096", "GSM2382097", "GSM2382098", "GSM2382099", "GSM2382100", "GSM2382101", "GSM2382102", "GSM2382103", "GSM2382104", "GSM2382105", "GSM2382106", "GSM2382107", "GSM2382108", "GSM2382109", "GSM2382110", "GSM2382111", "GSM2382112", "GSM2382113", "GSM2382114", "GSM2382115", "GSM2382116", "GSM2382117", "GSM2382118", "GSM2382119", "GSM2382120", "GSM2382121", "GSM2382122", "GSM2382123", "GSM2382124", "GSM2382125", "GSM2382126", "GSM2382127", "GSM2382128", "GSM2382129", "GSM2382130", "GSM2382131", "GSM2382132", "GSM2382133", "GSM2382134", "GSM2382135", "GSM2382136", "GSM2382137", "GSM2382138", "GSM2382139", "GSM2382140", "GSM2382141", "GSM2382142", "GSM2382143", "GSM2382144", "GSM2382145", "GSM2382146", "GSM2382147", "GSM2382148", "GSM2382149", "GSM2382150", "GSM2382151", "GSM2382152", "GSM2382153", "GSM2382154", "GSM2382155", "GSM2382156", "GSM2382157", "GSM2382158", "GSM2382159", "GSM2382160", "GSM2382161", "GSM2382162", "GSM2382163", "GSM2382164", "GSM2382165", "GSM2382166", "GSM2382167", "GSM2382168", "GSM2382169", "GSM2382170", "GSM2382171", "GSM2382172", "GSM2382173", "GSM2382174", "GSM2382175", "GSM2382176", "GSM2382177", "GSM2382178", "GSM2382179", "GSM2382180", "GSM2382181", "GSM2382182", "GSM2382183", "GSM2382184", "GSM2382185", "GSM2382186", "GSM2382187", "GSM2382188", "GSM2382189", "GSM2382190", "GSM2382191", "GSM2382192", "GSM2382193", "GSM2382194", "GSM2382195", "GSM2382196", "GSM2382197", "GSM2382198", "GSM2382199", "GSM2382200", "GSM2382201", "GSM2382202", "GSM2382203", "GSM2382204", "GSM2382205", "GSM2382206", "GSM2382207", "GSM2382208", "GSM2382209", "GSM2382210", "GSM2382211", "GSM2382212", "GSM2382213", "GSM2382214", "GSM2382215", "GSM2382216", "GSM2382217", "GSM2382218", "GSM2382219", "GSM2382220", "GSM2382221", "GSM2382222", "GSM2382223", "GSM2382224", "GSM2382225", "GSM2382226", "GSM2382227", "GSM2382228", "GSM2382229", "GSM2382230", "GSM2382231", "GSM2382232", "GSM2382233", "GSM2382234", "GSM2382235", "GSM2382236", "GSM2382237", "GSM2382238", "GSM2382239", "GSM2382240", "GSM2382241", "GSM2382242", "GSM2382243", "GSM2382244", "GSM2382245", "GSM2382246", "GSM2382247", "GSM2382248", "GSM2382249", "GSM2382250", "GSM2382251", "GSM2382252", "GSM2382253", "GSM2382254", "GSM2382255", "GSM2382256", "GSM2382257", "GSM2382258", "GSM2382259", "GSM2382260", "GSM2382261", "GSM2382262", "GSM2382263", "GSM2382264", "GSM2382265", "GSM2382266", "GSM2382267", "GSM2382268", "GSM2382269", "GSM2382270", "GSM2382271", "GSM2382272", "GSM2382273", "GSM2382274", "GSM2382275", "GSM2382276", "GSM2382277", "GSM2382278", "GSM2382279", "GSM2382280", "GSM2382281", "GSM2382282", "GSM2382283", "GSM2382284", "GSM2382285", "GSM2382286", "GSM2382287", "GSM2382288", "GSM2382289", "GSM2382290", "GSM2382291", "GSM2382292", "GSM2382293", "GSM2382294", "GSM2382295", "GSM2382296", "GSM2382297", "GSM2382298", "GSM2382299", "GSM2382300", "GSM2382301", "GSM2382302", "GSM2382303", "GSM2382304", "GSM2382305", "GSM2382306", "GSM2382307", "GSM2382308", "GSM2382309", "GSM2382310", "GSM2382311", "GSM2382312", "GSM2382313", "GSM2382314", "GSM2382315", "GSM2382316", "GSM2382317", "GSM2382318", "GSM2382319", "GSM2382320", "GSM2382321", "GSM2382322", "GSM2382323", "GSM2382324", "GSM2382325", "GSM2382326", "GSM2382327", "GSM2382328", "GSM2382329", "GSM2382330", "GSM2382331", "GSM2382332", "GSM2382333", "GSM2382334", "GSM2382335", "GSM2382336", "GSM2382337", "GSM2382338", "GSM2382339", "GSM2382340", "GSM2382341", "GSM2382342", "GSM2382343", "GSM2382344", "GSM2382345", "GSM2382346", "GSM2382347", "GSM2382348", "GSM2382349", "GSM2382350", "GSM2382351", "GSM2382352", "GSM2382353", "GSM2382354", "GSM2382355", "GSM2382356", "GSM2382357", "GSM2382358", "GSM2382359", "GSM2382360", "GSM2382361", "GSM2382362", "GSM2382363", "GSM2382364", "GSM2382365", "GSM2382366", "GSM2382367", "GSM2382368", "GSM2382369", "GSM2382370", "GSM2382371", "GSM2382372", "GSM2382373", "GSM2382374", "GSM2382375", "GSM2382376", "GSM2382377", "GSM2382378", "GSM2382379", "GSM2382380", "GSM2382381", "GSM2382382", "GSM2382383", "GSM2382384", "GSM2382385", "GSM2382386", "GSM2382387", "GSM2382388", "GSM2382389", "GSM2382390", "GSM2382391", "GSM2382392", "GSM2382393", "GSM2382394", "GSM2382395", "GSM2382396", "GSM2382397", "GSM2382398", "GSM2382399", "GSM2382400", "GSM2382401", "GSM2382402", "GSM2382403", "GSM2382404", "GSM2382405", "GSM2382406", "GSM2382407", "GSM2382408", "GSM2382409", "GSM2382410", "GSM2382411", "GSM2382412", "GSM2382413", "GSM2382414", "GSM2382415", "GSM2382416", "GSM2382417", "GSM2382418", "GSM2382419", "GSM2382420", "GSM2382421", "GSM2382422", "GSM2382423", "GSM2382424", "GSM2382425", "GSM2382426", "GSM2382427", "GSM2382428", "GSM2382429", "GSM2382430", "GSM2382431", "GSM2382432", "GSM2382433", "GSM2382434", "GSM2382435", "GSM2382436", "GSM2382437", "GSM2382438", "GSM2382439", "GSM2382440", "GSM2382441", "GSM2382442", "GSM2382443", "GSM2382444", "GSM2382445", "GSM2382446", "GSM2382447", "GSM2382448", "GSM2382449", "GSM2382450", "GSM2382451", "GSM2382452", "GSM2382453", "GSM2382454", "GSM2382455", "GSM2382456", "GSM2382457", "GSM2382458", "GSM2382459", "GSM2382460", "GSM2382461", "GSM2382462", "GSM2382463", "GSM2382464", "GSM2382465", "GSM2382466", "GSM2382467", "GSM2382468", "GSM2382469", "GSM2382470", "GSM2382471", "GSM2382472", "GSM2382473", "GSM2382474", "GSM2382475", "GSM2382476", "GSM2382477", "GSM2382478", "GSM2382479", "GSM2382480", "GSM2382481", "GSM2382482", "GSM2382483", "GSM2382484", "GSM2382485", "GSM2382486", "GSM2382487", "GSM2382488", "GSM2382489", "GSM2382490", "GSM2382491", "GSM2382492", "GSM2382493", "GSM2382494", "GSM2382495", "GSM2382496", "GSM2382497", "GSM2382498", "GSM2382499", "GSM2382500", "GSM2382501", "GSM2382502", "GSM2382503", "GSM2382504", "GSM2382505", "GSM2382506", "GSM2382507", "GSM2382508", "GSM2382509", "GSM2382510", "GSM2382511", "GSM2382512", "GSM2382513", "GSM2382514", "GSM2382515", "GSM2382516", "GSM2382517", "GSM2382518", "GSM2382519", "GSM2382520", "GSM2382521", "GSM2382522", "GSM2382523", "GSM2382524", "GSM2382525", "GSM2382526", "GSM2382527", "GSM2382528", "GSM2382529", "GSM2382530", "GSM2382531", "GSM2382532", "GSM2382533", "GSM2382534", "GSM2382535", "GSM2382536", "GSM2382537", "GSM2382538", "GSM2382539", "GSM2382540", "GSM2382541", "GSM2382542", "GSM2382543", "GSM2382544", "GSM2382545", "GSM2382546", "GSM2382547", "GSM2382548", "GSM2382549", "GSM2382550", "GSM2382551", "GSM2382552", "GSM2382553", "GSM2382554", "GSM2382555", "GSM2382556", "GSM2382557", "GSM2382558", "GSM2382559", "GSM2382560", "GSM2382561", "GSM2382562", "GSM2382563", "GSM2382564", "GSM2382565", "GSM2382566", "GSM2382567", "GSM2382568", "GSM2382569", "GSM2382570", "GSM2382571", "GSM2382572", "GSM2382573", "GSM2382574", "GSM2382575", "GSM2382576", "GSM2382577", "GSM2382578", "GSM2382579", "GSM2382580", "GSM2382581", "GSM2382582", "GSM2382583", "GSM2382584", "GSM2382585", "GSM2382586", "GSM2382587", "GSM2382588", "GSM2382589", "GSM2382590", "GSM2382591", "GSM2382592", "GSM2382593", "GSM2382594", "GSM2382595", "GSM2382596", "GSM2382597", "GSM2382598", "GSM2382599", "GSM2382600", "GSM2382601", "GSM2382602", "GSM2382603", "GSM2382604", "GSM2382605", "GSM2382606", "GSM2382607", "GSM2382608", "GSM2382609", "GSM2382610", "GSM2382611", "GSM2382612", "GSM2382613", "GSM2382614", "GSM2382615", "GSM2382616", "GSM2382617", "GSM2382618", "GSM2382619", "GSM2382620", "GSM2382621", "GSM2382622", "GSM2382623", "GSM2382624", "GSM2382625", "GSM2382626", "GSM2382627", "GSM2382628", "GSM2382629", "GSM2382630", "GSM2382631", "GSM2382632", "GSM2382633", "GSM2382634", "GSM2382635", "GSM2382636", "GSM2382637", "GSM2382638", "GSM2382639", "GSM2382640", "GSM2382641", "GSM2382642", "GSM2382643", "GSM2382644", "GSM2382645", "GSM2382646", "GSM2382647", "GSM2382648", "GSM2382649", "GSM2382650", "GSM2382651", "GSM2382652", "GSM2382653", "GSM2382654", "GSM2382655", "GSM2382656", "GSM2382657", "GSM2382658", "GSM2382659", "GSM2382660", "GSM2382661", "GSM2382662", "GSM2382663", "GSM2382664", "GSM2382665", "GSM2382666", "GSM2382667", "GSM2382668", "GSM2382669", "GSM2382670", "GSM2382671", "GSM2382672", "GSM2382673", "GSM2382674", "GSM2382675", "GSM2382676", "GSM2382677", "GSM2382678", "GSM2382679", "GSM2382680", "GSM2382681", "GSM2382682", "GSM2382683", "GSM2382684", "GSM2382685", "GSM2382686", "GSM2382687", "GSM2382688", "GSM2382689", "GSM2382690", "GSM2382691", "GSM2382692", "GSM2382693", "GSM2382694", "GSM2382695", "GSM2382696", "GSM2382697", "GSM2382698", "GSM2382699", "GSM2382700", "GSM2382701", "GSM2382702", "GSM2382703", "GSM2382704", "GSM2382705", "GSM2382706", "GSM2382707", "GSM2382708", "GSM2382709", "GSM2382710", "GSM2382711", "GSM2382712", "GSM2382713", "GSM2382714", "GSM2382715", "GSM2382716", "GSM2382717", "GSM2382718", "GSM2382719", "GSM2382720", "GSM2382721", "GSM2382722", "GSM2382723", "GSM2382724", "GSM2382725", "GSM2382726", "GSM2382727", "GSM2382728", "GSM2382729", "GSM2382730", "GSM2382731", "GSM2382732", "GSM2382733", "GSM2382734", "GSM2382735", "GSM2382736", "GSM2382737", "GSM2382738", "GSM2382739", "GSM2382740", "GSM2382741", "GSM2382742", "GSM2382743", "GSM2382744", "GSM2382745", "GSM2382746", "GSM2382747", "GSM2382748", "GSM2382749", "GSM2382750", "GSM2382751", "GSM2382752", "GSM2382753", "GSM2382754", "GSM2382755", "GSM2382756", "GSM2382757", "GSM2382758", "GSM2382759", "GSM2382760", "GSM2382761", "GSM2382762", "GSM2382763", "GSM2382764", "GSM2382765", "GSM2382766", "GSM2382767", "GSM2382768", "GSM2382769", "GSM2382770", "GSM2382771", "GSM2382772", "GSM2382773", "GSM2382774", "GSM2382775", "GSM2382776", "GSM2382777", "GSM2382778", "GSM2382779", "GSM2382780", "GSM2382781", "GSM2382782", "GSM2382783", "GSM2382784", "GSM2382785", "GSM2382786", "GSM2382787", "GSM2382788", "GSM2382789", "GSM2382790", "GSM2382791", "GSM2382792", "GSM2382793", "GSM2382794", "GSM2382795", "GSM2382796", "GSM2382797", "GSM2382798", "GSM2382799", "GSM2382800", "GSM2382801", "GSM2382802", "GSM2382803", "GSM2382804", "GSM2382805", "GSM2382806", "GSM2382807", "GSM2382808", "GSM2382809", "GSM2382810", "GSM2382811", "GSM2382812", "GSM2382813", "GSM2382814", "GSM2382815", "GSM2382816", "GSM2382817", "GSM2382818", "GSM2382819", "GSM2382820", "GSM2382821", "GSM2382822", "GSM2382823", "GSM2382824", "GSM2382825", "GSM2382826", "GSM2382827", "GSM2382828", "GSM2382829", "GSM2382830", "GSM2382831", "GSM2382832", "GSM2382833", "GSM2382834", "GSM2382835", "GSM2382836", "GSM2382837", "GSM2382838", "GSM2382839", "GSM2382840", "GSM2382841", "GSM2382842", "GSM2382843", "GSM2382844", "GSM2382845", "GSM2382846", "GSM2382847", "GSM2382848", "GSM2382849", "GSM2382850", "GSM2382851", "GSM2382852", "GSM2382853", "GSM2382854", "GSM2382855", "GSM2382856", "GSM2382857", "GSM2382858", "GSM2382859", "GSM2382860", "GSM2382861", "GSM2382862", "GSM2382863", "GSM2382864", "GSM2382865", "GSM2382866", "GSM2382867", "GSM2382868", "GSM2382869", "GSM2382870", "GSM2382871", "GSM2382872", "GSM2382873", "GSM2382874", "GSM2382875", "GSM2382876", "GSM2382877", "GSM2382878", "GSM2382879", "GSM2382880", "GSM2382881", "GSM2382882", "GSM2382883", "GSM2382884", "GSM2382885", "GSM2382886", "GSM2382887", "GSM2382888", "GSM2382889", "GSM2382890", "GSM2382891", "GSM2382892", "GSM2382893", "GSM2382894", "GSM2382895", "GSM2382896", "GSM2382897", "GSM2382898", "GSM2382899", "GSM2382900", "GSM2382901", "GSM2382902", "GSM2382903", "GSM2382904", "GSM2382905", "GSM2382906", "GSM2382907", "GSM2382908", "GSM2382909", "GSM2382910", "GSM2382911", "GSM2382912", "GSM2382913", "GSM2382914", "GSM2382915", "GSM2382916", "GSM2382917", "GSM2382918", "GSM2382919", "GSM2382920", "GSM2382921", "GSM2382922", "GSM2382923", "GSM2382924", "GSM2382925", "GSM2382926", "GSM2382927", "GSM2382928", "GSM2382929", "GSM2382930", "GSM2382931", "GSM2382932", "GSM2382933", "GSM2382934", "GSM2382935", "GSM2382936", "GSM2382937", "GSM2382938", "GSM2382939", "GSM2382940", "GSM2382941", "GSM2382942", "GSM2382943", "GSM2382944", "GSM2382945", "GSM2382946", "GSM2382947", "GSM2382948", "GSM2382949", "GSM2382950", "GSM2382951", "GSM2382952", "GSM2382953", "GSM2382954", "GSM2382955", "GSM2382956", "GSM2382957", "GSM2382958", "GSM2382959", "GSM2382960", "GSM2382961", "GSM2382962", "GSM2382963", "GSM2382964", "GSM2382965", "GSM2382966", "GSM2382967", "GSM2382968", "GSM2382969", "GSM2382970", "GSM2382971", "GSM2382972", "GSM2382973", "GSM2382974", "GSM2382975", "GSM2382976", "GSM2382977", "GSM2382978", "GSM2382979", "GSM2382980", "GSM2382981", "GSM2382982", "GSM2382983", "GSM2382984", "GSM2382985", "GSM2382986", "GSM2382987", "GSM2382988", "GSM2382989", "GSM2382990", "GSM2382991", "GSM2382992", "GSM2382993", "GSM2382994", "GSM2382995", "GSM2382996", "GSM2382997", "GSM2382998", "GSM2382999", "GSM2383000", "GSM2383001", "GSM2383002", "GSM2383003", "GSM2383004", "GSM2383005", "GSM2383006", "GSM2383007", "GSM2383008", "GSM2383009", "GSM2383010", "GSM2383011", "GSM2383012", "GSM2383013", "GSM2383014", "GSM2383015", "GSM2383016", "GSM2383017", "GSM2383018", "GSM2383019", "GSM2383020", "GSM2383021", "GSM2383022", "GSM2383023", "GSM2383024", "GSM2383025", "GSM2383026", "GSM2383027", "GSM2383028", "GSM2383029", "GSM2383030", "GSM2383031", "GSM2383032", "GSM2383033", "GSM2383034", "GSM2383035", "GSM2383036", "GSM2383037", "GSM2383038", "GSM2383039", "GSM2383040", "GSM2383041", "GSM2383042", "GSM2383043", "GSM2383044", "GSM2383045", "GSM2383046", "GSM2383047", "GSM2383048", "GSM2383049", "GSM2383050", "GSM2383051", "GSM2383052", "GSM2383053", "GSM2383054", "GSM2383055", "GSM2383056", "GSM2383057", "GSM2383058", "GSM2383059", "GSM2383060", "GSM2383061", "GSM2383062", "GSM2383063", "GSM2383064", "GSM2383065", "GSM2383066", "GSM2383067", "GSM2383068", "GSM2383069", "GSM2383070", "GSM2383071", "GSM2383072", "GSM2383073", "GSM2383074", "GSM2383075", "GSM2383076", "GSM2383077", "GSM2383078", "GSM2383079", "GSM2383080", "GSM2383081", "GSM2383082", "GSM2383083", "GSM2383084", "GSM2383085", "GSM2383086", "GSM2383087", "GSM2383088", "GSM2383089", "GSM2383090", "GSM2383091", "GSM2383092", "GSM2383093", "GSM2383094", "GSM2383095", "GSM2383096", "GSM2383097", "GSM2383098", "GSM2383099", "GSM2383100", "GSM2383101", "GSM2383102", "GSM2383103", "GSM2383104", "GSM2383105", "GSM2383106", "GSM2383107", "GSM2383108", "GSM2383109", "GSM2383110", "GSM2383111", "GSM2383112", "GSM2383113", "GSM2383114", "GSM2383115", "GSM2383116", "GSM2383117", "GSM2383118", "GSM2383119", "GSM2383120", "GSM2383121", "GSM2383122", "GSM2383123", "GSM2383124", "GSM2383125", "GSM2383126", "GSM2383127", "GSM2383128", "GSM2383129", "GSM2383130", "GSM2383131", "GSM2383132", "GSM2383133", "GSM2383134", "GSM2383135", "GSM2383136", "GSM2383137", "GSM2383138", "GSM2383139", "GSM2383140", "GSM2383141", "GSM2383142", "GSM2383143", "GSM2383144", "GSM2383145", "GSM2383146", "GSM2383147", "GSM2383148", "GSM2383149", "GSM2383150", "GSM2383151", "GSM2383152", "GSM2383153", "GSM2383154", "GSM2383155", "GSM2383156", "GSM2383157", "GSM2383158", "GSM2383159", "GSM2383160", "GSM2383161", "GSM2383162", "GSM2383163", "GSM2383164", "GSM2383165", "GSM2383166", "GSM2383167", "GSM2383168", "GSM2383169", "GSM2383170", "GSM2383171", "GSM2383172", "GSM2383173", "GSM2383174", "GSM2383175", "GSM2383176", "GSM2383177", "GSM2383178", "GSM2383179", "GSM2383180", "GSM2383181", "GSM2383182", "GSM2383183", "GSM2383184", "GSM2383185", "GSM2383186", "GSM2383187", "GSM2383188", "GSM2383189", "GSM2383190", "GSM2383191", "GSM2383192", "GSM2383193", "GSM2383194", "GSM2383195", "GSM2383196", "GSM2383197", "GSM2383198", "GSM2383199", "GSM2383200", "GSM2383201", "GSM2383202", "GSM2383203", "GSM2383204", "GSM2383205", "GSM2383206", "GSM2383207", "GSM2383208", "GSM2383209", "GSM2383210", "GSM2383211", "GSM2383212", "GSM2383213", "GSM2383214", "GSM2383215", "GSM2383216", "GSM2383217", "GSM2383218", "GSM2383219", "GSM2383220", "GSM2383221", "GSM2383222", "GSM2383223", "GSM2383224", "GSM2383225", "GSM2383226", "GSM2383227", "GSM2383228", "GSM2383229", "GSM2383230", "GSM2383231", "GSM2383232", "GSM2383233", "GSM2383234", "GSM2383235", "GSM2383236", "GSM2383237", "GSM2383238", "GSM2383239", "GSM2383240", "GSM2383241", "GSM2383242", "GSM2383243", "GSM2383244", "GSM2383245", "GSM2383246", "GSM2383247", "GSM2383248", "GSM2383249", "GSM2383250", "GSM2383251", "GSM2383252", "GSM2383253", "GSM2383254", "GSM2383255", "GSM2383256", "GSM2383257", "GSM2383258", "GSM2383259", "GSM2383260", "GSM2383261", "GSM2383262", "GSM2383263", "GSM2383264", "GSM2383265", "GSM2383266", "GSM2383267", "GSM2383268", "GSM2383269", "GSM2383270", "GSM2383271", "GSM2383272", "GSM2383273", "GSM2383274", "GSM2383275", "GSM2383276", "GSM2383277", "GSM2383278", "GSM2383279", "GSM2383280", "GSM2383281", "GSM2383282", "GSM2383283", "GSM2383284", "GSM2383285", "GSM2383286", "GSM2383287", "GSM2383288", "GSM2383289", 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"GSM2383858", "GSM2383859", "GSM2383860", "GSM2383861", "GSM2383862", "GSM2383863", "GSM2383864", "GSM2383865", "GSM2383866", "GSM2383867", "GSM2383868", "GSM2383869", "GSM2383870", "GSM2383871", "GSM2383872", "GSM2383873", "GSM2383874", "GSM2383875", "GSM2383876", "GSM2383877", "GSM2383878", "GSM2383879", "GSM2383880", "GSM2383881", "GSM2383882", "GSM2383883", "GSM2383884", "GSM2383885", "GSM2383886", "GSM2383887", "GSM2383888", "GSM2383889", "GSM2383890", "GSM2383891", "GSM2383892", "GSM2383893", "GSM2383894", "GSM2383895", "GSM2383896", "GSM2383897", "GSM2383898", "GSM2383899", "GSM2383900", "GSM2383901", "GSM2383902", "GSM2383903", "GSM2383904", "GSM2383905", "GSM2383906", "GSM2383907", "GSM2383908", "GSM2383909", "GSM2383910", "GSM2383911"], "sample_count": 6341, "group_sizes": null, "group_sizes_basis": null, "platform": "18573", "taxon": "Homo sapiens", "title": "Single cell RNA-seq analysis of IDH-mutant astrocytoma", "resolution_checked_at": "2026-11-01T18:46:34Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:GSE84465 not downloaded", "asset:data:geo:GSE84465 licence not read", "asset:data:geo:GSE89567 not downloaded", "asset:data:geo:GSE89567 licence not read"]}, "analysis": {"state": "complete", "methods": [{"id": "method-1", "family": "survival_analysis", "specific": "kaplan_meier", "label": "Survival analysis", "dsl_operations": ["survival_km"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["time_to_event_with_censoring"], "evidence": {"score": 1.0, "band": "verbatim", "quote": "Kaplan-Meier (KM) was utilized to compare HR and LR patient survival via the Log-rank."}}, {"id": "method-2", "family": "survival_analysis", "specific": "log_rank_test", "label": "Survival analysis", "dsl_operations": ["logrank_test"], "missing_operations": [], "expressible": true, "parameters": {"hazard_ratio": "was utilized to compare HR and LR patient survival"}, "data_shapes": ["time_to_event_with_censoring"], "evidence": {"score": 0.8, "band": "high", "quote": "Log-rank"}}, {"id": "method-3", "family": "principal_component_analysis", "specific": "pca", "label": "Principal component analysis", "dsl_operations": ["pca_fit"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["wide_numeric_matrix"], "evidence": {"score": 0.8, "band": "high", "quote": "Principal component analysis"}}, {"id": "method-4", "family": "differential_expression", "specific": "limma_voom", "label": "Differential expression analysis", "dsl_operations": ["normalise_counts", "differential_expression_fit", "fdr_bh"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["count_matrix_with_condition"], "evidence": {"score": 0.8, "band": "high", "quote": "limma"}}], "targets": [], "gaps": []}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "complete", "tier": "R1", "blocking_axis": "none", "blocked_on": "nothing:replicable_now", "axes": {"method": {"tier": "R1", "expressible": true, "required_operations": ["survival_km"], "missing_operations": [], "basis": "The paper names the method it used and the corpus's method extractor maps it onto the DSL catalogue: kaplan_meier."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R1", "reachable": true, "accessions": ["GSE89567", "GSE84465"], "resolved_accessions": ["GSE89567", "GSE84465"], "statements": ["Data are available in a public, open access repository. The datasets analyzed during the current study are available in the Gene Expression Omnibus (GSE89567: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE89567; GSE84465: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84465) and The Cancer Genome Atlas (TCGA) (https://cancergenome.nih.gov/) and the Chinese Glioma Genome Atlas (http://www.cgga.org.cn/)."], "basis": "The paper names GSE89567, GSE84465 in Gene Expression Omnibus and the accession resolves in the repository. No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": ["survival_km"], "assets": ["source:pmid:36389681", "data:geo:GSE84465"], "steps": ["run_analysis"]}, "must_build": {"dsl_operations": [], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:GSE84465"]}, "gaps": []}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-11-01T18:15:53Z", "independence": "single_reader"}, "evidence_check": {"method": "verbatim", "availability": "verbatim"}, "calls": 5, "usage": {"prompt_tokens": 8633, "completion_tokens": 1876, "cached_tokens": 264}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:36389681", "type": "paper", "label": "pmid:36389681"}, {"id": "source:pmid:36389681", "type": "manuscript", "label": "PMID 36389681 full text"}, {"id": "data:geo:GSE84465", "type": "dataset", "label": "GEO GSE84465"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:36389681", "type": "paper", "label": "pmid:36389681"}, {"id": "source:pmid:36389681", "type": "manuscript", "label": "PMID 36389681 full text"}, {"id": "data:geo:GSE84465", "type": "dataset", "label": "GEO GSE84465"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-36389681", "type": "extraction", "used": ["source:pmid:36389681"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-GSE84465", "type": "accession_resolution", "used": ["data:geo:GSE84465"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:36389681", "parent_hash": "sha256:cd8b83b724100cc3263f30c6818119f72a54262d6fe9021dd0d9781cfb92f1b8", "fork_of": null, "generation": 3}, "gaps": ["asset:data:geo:GSE84465 licence not read", "asset:data:geo:GSE84465 not downloaded", "asset:data:geo:GSE89567 licence not read", "asset:data:geo:GSE89567 not downloaded", "execution.not_run", "review.human_critique", "review.independent_reader", "review.machine_critique"], "assays": [{"role": "data_generation", "definition_id": "scrna_seq", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "unsupported", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["hods and materials Patients and datasets The scRNA-seq data of 26 glioma cases, including 13 LGG an", "rsity. Processing and analysis of the glioma scRNA-seq data The Seurat R package was employed for s", "q data The Seurat R package was employed for scRNA-seq data analysis (12). Quality control was achi", "ponent analysis (PCA) was employed to reduce scRNA-seq data dimension (13). In short, 30 principal"], "order_basis": "paper_text_order:2086", "id": "assay:1"}, {"role": "data_generation", "definition_id": "rna_seq", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["(CNAs) that corresponded with the cases with RNA-seq data. Next, we utilized the R software packa", "supplemented in Table S1 . Subsequently, the RNA sequencing data were normalized. In a dataset that had", "to computation via RPKM (reads per kilobase transcriptome per million reads) (11). Overall survival (O"], "order_basis": "paper_text_order:2552", "id": "assay:2"}, {"role": "analysis", "definition_id": "survival_analysis", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["survival_km"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Kaplan-Meier (KM) was utilized to compare HR and LR patient survival via the Log-rank."], "order_basis": "consumes_earlier_output", "id": "assay:3"}]}