Potential impact of WTAP and YTHDF2 on tumor immunity in lung adenocarcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36397411", "kind": "science-record", "revision": 3, "content_hash": "sha256:b5e168f1c9816d8c7194bdc1f130b69f5d9c698f40155f3a471cb13f2ab8ad51", "created_at": "2026-11-01T19:00:51Z", "updated_at": "2026-11-01T22:08:06Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Potential impact of WTAP and YTHDF2 on tumor immunity in lung adenocarcinoma.", "doi": "10.1097/MD.0000000000031195", "pmid": "36397411", "authors": ["Xinyu Zhang", "Xinsheng Cai"], "journal": "Medicine", "year": 2022, "abstract": "WTAP and N6-methyladenosine (m6A) reader proteins (YTHDF2) are N6-methyladenosine (m6A) methyltransferase and m6A reading proteins, respectively. In recent years, the tumor immune environment has received more and more attention in the progress and treatment of cancer. The aim of this study was to investigate the relationship between N6-methyladenosine (m6A) methyltransferase (WTAP)/YTHDF2 and the immunological characteristics of lung adenocarcinoma (LUAD). Based on the expression of WTAP and YTHDF2 in the cancer genome atlas (TCGA) and gene expression omnibus (GEO) database, LUAD patients were divided into 2 clusters by coherently clustering method, and performed gene set enrichment analysis (GSEA) to identify the functional differences. Immunoinvasion analysis was performed using ESTIMATE, CIBERSORT, and single-sample GSEA (ssGSEA), and expression of immune checkpoint inhibitors (ICIs) targets was assessed, while tumor mutation burden (TMB) was calculated in tumor samples. Weighted gene co-expression network analysis (WGCNA) was used to identify the genes related to both WTAP/YTHDF2 expression and immunity. The immunological characteristics between the 2 clusters were externally verified based on GSE39582. The expression of WTAP was higher in cluster 1 and YTHDF2 was lower, but it was opposite in cluster 2. Cluster 1 had stronger immune infiltration, more ICIs target expression, more TMB. In addition, WGCNA identified 22 genes associated with WTAP/YTHDF2 expression and immune score, including TIM3 (HAVCR2) and CD86. WTAP and YTHDF2 influence immune contexture and may be novel prognostic and druggable targets associated with the immune system of LUAD.", "claims": [{"id": "claim-method", "statement": "Box plot analysis was performed using Wilcoxon rank sum test. Correlation analysis was performed using Spearman’s correlation analysis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36397411", "locator": "sections[11] \"2.9. Statistical analysis\" paragraph 2", "quote": "Box plot analysis was performed using Wilcoxon rank sum test. Correlation analysis was performed using Spearman’s correlation analysis."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets generated during and/or analyzed during the current study are publicly available.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36397411", "locator": "sections[28] \"(untitled)\" paragraph 1", "quote": "The datasets generated during and/or analyzed during the current study are publicly available."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Cluster 1 had stronger immune infiltration, more ICI-target expression, and higher tumor mutational burden than cluster 2.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36397411", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Cluster 1 had stronger immune infiltration, more ICIs target expression, more TMB."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "Cluster 1 had stronger immune infiltration, more ICI-target expression, and higher tumor mutational burden than cluster 2.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36397411"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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