Bioinformatic analysis of the RNA expression patterns in microgravity-induced bone loss.

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However, the underlying molecular mechanisms are still unclear, which hinders the development of therapeutic measures. The aim of this study was to identify hub genes and explore novel molecular mechanisms underlying microgravity-induced bone loss using transcriptome datasets obtained from the GEO and SRA databases. In summary, comparative RNA expression pattern studies that differ in species (Homo or Mus), models (in vitro or in vivo), microgravity conditions (real microgravity or ground-based simulators) and microgravity duration showed that it is difficult to reach a consistent conclusion about the pathogenesis of microgravity-induced bone loss across these studies. Even so, we identified 11 hub genes and some miRNA-mRNA interactions mainly based on the GSE100930 dataset. Also, the expression of CCL2, ICAM1, IGF1, miR-101-3p and miR-451a markedly changed under clinorotation-microgravity condition. Remarkedly, ICAM1 and miR-451a were key mediators of the osteogenesis of hMSCs under clinorotation-microgravity condition. These findings provide novel insights into the molecular mechanisms of bone loss during microgravity and could indicate potential targets for further countermeasures against this condition.", "claims": [{"id": "claim-method", "statement": "Then, DEGs were screened out by “Limma” with a p value <0.05 and |log2FC| > 1.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36425065", "locator": "sections[4] \"Raw data processing and identification of DEGs\" paragraph 2", "quote": "Then, DEGs were screened out by “Limma” with a p value <0.05 and |log2FC| > 1."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding authors.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36425065", "locator": "sections[29] \"Data availability statement\" paragraph 2", "quote": "The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding authors."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study identified 11 hub genes and miRNA-mRNA interactions mainly from the GSE100930 dataset.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36425065", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Even so, we identified 11 hub genes and some miRNA-mRNA interactions mainly based on the GSE100930 dataset."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study identified 11 hub genes and miRNA-mRNA interactions mainly from the GSE100930 dataset.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36425065"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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