HSPA8 Is a New Biomarker of Triple Negative Breast Cancer Related to Prognosis and Immune Infiltration.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36452344", "kind": "science-record", "revision": 3, "content_hash": "sha256:8026e7f4b2268b88d6a6d42470d1cbb939d24e61a1d9ff6446ac893a4285d7b3", "created_at": "2026-11-01T19:00:56Z", "updated_at": "2026-11-01T22:50:43Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "HSPA8 Is a New Biomarker of Triple Negative Breast Cancer Related to Prognosis and Immune Infiltration.", "doi": "10.1155/2022/8446857", "pmid": "36452344", "authors": ["Bicheng Ying", "Wenting Xu", "Yan Nie", "Yongtao Li"], "journal": "Disease markers", "year": 2022, "abstract": "Objective: Triple negative breast cancer (TNBC) is a kind of cancer that endangers the lives of women all over the world in the 21st century. Heat shock protein member 8 (HSPA8) is the chaperone gene of the heat shock protein family. It is involved in many cellular functions. For example, it promotes the circulation between ATP and ADP, participates in protein folding, and can change the vitality of the cell and inhibit its growth. However, the abnormal expression of HSPA8 gene in TNBC and its diagnostic and prognostic significance still need to be further studied.\nMethods: First, we used related databases (such as TCGA, GEO, GTEx, ONCOMINE, TIMER2.0, UALCAN, HPA, STRING, CCLE, and Kaplan-Meier plotter databases) to analyze the relationship between HSPA8 and TNBC by bioinformatics. Then, the analysis using only a small part of the experimental work is used to explain our findings. For example, HSPA8 protein expression was evaluated by immunohistochemical method in TNBC tissues. Western blotting experiments were carried out to verify the results. Then, the clinicopathological characteristics of patients with TNBC were analyzed by R software and Cox regression analysis. On the basis, a nomogram is constructed to estimate the 1-, 3-, and 5-year overall survival (OS). The prognostic nomogram performance was calibrated and evaluated by the calibration curve and receiver operating characteristic (ROC) curve.\nResults: In the study, we analyzed the three GEO databases (including GSE86945, GSE106977, and GSE102088) and found that HSPA8 is one of the central genes of TNBC. Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) researches indicated that HSPA8 was mainly involved in partner-mediated autophagy, mRNA catabolism, neutrophil activation, immune response, protein targeting, RNA splicing, RNA catabolism, and other biological processes. Next, we used bioinformatics technology to find that the expression level of HSPA8 in breast cancer (BC) and TNBC samples was significantly higher than that in normal breast tissues, which was determined by analyzing hospital patient samples and related experiments. In addition, the expression level of HSPA8 in BC and TNBC samples was significantly correlated with clinical indexes such as TNM stage. The Cox analysis revealed that the expression of HSPA8 in TNBC had significant clinical prognostic value. The results of nomogram and ROC test show that HSPA8 has significant predictive ability in TNBC. The results of immune infiltration of HSPA8 through the TIMER2.0 database showed that there was a significant correlation between HSPA8 and immune cell subsets.\nConclusions: Our results show that the expression of HSPA8 in TNBC has important clinical diagnostic significance and clarify the potential molecular mechanism that promotes the evolution of TNBC. The high expression of HSPA8 may be related with the poor clinical outcome of TNBC. This helps to provide us with a new direction of TNBC targeted therapy.", "claims": [{"id": "claim-method", "statement": "The Kaplan-Meier analysis was employed for evaluating patient survival. The purpose of Cox regression was to understand the significance of HSPA8 expression and other clinical parameters in patients with TNBC. The ROC was established by using “PROC package” to analyze the HSPA8 expression significance in diagnosis", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36452344", "locator": "sections[11] \"2.9. Statistical Analysis\" paragraph 2", "quote": "The Kaplan-Meier analysis was employed for evaluating patient survival. The purpose of Cox regression was to understand the significance of HSPA8 expression and other clinical parameters in patients with TNBC. The ROC was established by using “PROC package” to analyze the HSPA8 expression significance in diagnosis"}], "state": "complete"}, {"id": "claim-data-access", "statement": "The ONCOMINE, TIMER2.0, UALCAN, HPA, STRING, CCLE, and Kaplan-Meier plotter databases used to support the findings of this study are included within the article. And the HSPA8-Clinical data and TCGA-TNBC-data used to support the findings of this study are included within the supplementary information file(s) (available here).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36452344", "locator": "sections[24] \"Data Availability\" paragraph 2", "quote": "The ONCOMINE, TIMER2.0, UALCAN, HPA, STRING, CCLE, and Kaplan-Meier plotter databases used to support the findings of this study are included within the article. And the HSPA8-Clinical data and TCGA-TNBC-data used to support the findings of this study are included within the supplementary information file(s) (available here)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "HSPA8 is highly expressed in TNBC and has diagnostic and prognostic significance, with high expression associated with poor clinical outcome.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36452344", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "Our results show that the expression of HSPA8 in TNBC has important clinical diagnostic significance and clarify the potential molecular mechanism that promotes the evolution of TNBC. The high expression of HSPA8 may be related with the poor clinical outcome of TNBC."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "HSPA8 is highly expressed in TNBC and has diagnostic and prognostic significance, with high expression associated with poor clinical outcome.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36452344"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE106977"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE106977"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:36452344", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1155/2022/8446857", "sha256": "sha256:ce27fa0aafaef705ef0ad15fbb0f6e906680322bb39a4d8fd2e3c5c3432c9657", "bytes": 36233, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1155/2022/8446857", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC9705114", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE106977", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE106977", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE106977", "version": null, "note": "The ONCOMINE, TIMER2.0, UALCAN, HPA, STRING, CCLE, and Kaplan-Meier plotter databases used to support the findings of this study are included within the article. 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