Low Expression of AGPAT5 Is Associated With Clinical Stage and Poor Prognosis in Colorectal Cancer and Contributes to Tumour Progression.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36468151", "kind": "science-record", "revision": 3, "content_hash": "sha256:d144ded336022d1a928d6d101a398103d3e394ff8895b64c60ab5650f64e6b15", "created_at": "2026-11-01T19:00:57Z", "updated_at": "2026-11-01T22:08:15Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Low Expression of AGPAT5 Is Associated With Clinical Stage and Poor Prognosis in Colorectal Cancer and Contributes to Tumour Progression.", "doi": "10.1177/11795549221137399", "pmid": "36468151", "authors": ["Jia Zang", "Juanjuan Sun", "WenChao Xiu", "Xiaoshuang Liu", "Yunsheng Chai", "Yanyan Zhou"], "journal": "Clinical Medicine Insights. Oncology", "year": 2022, "abstract": "Background: Colorectal cancer (CRC) has a high prevalence and poor prognosis. This study aimed to identify biomarkers related to the clinical stage (I-IV) of CRC.\nMethods: The LinkedOmics database was used as the discovery cohort, and two Gene Expression Omnibus (GEO) databases (GSE41258 and GSE422848) served as validation cohorts. The trend test of genes related to clinical stage (I-IV) of CRC patients was identified by the Jonckheere-Terpstra test. The cBioPortal database, Gene Expression Profiling Interactive Analysis (GEPIA) and PrognoScan databases were used to explore the expression change and prognostic value of clinical stage-related genes in CRC patients. CRC cells overexpressed AGPAT5 were constructed and used for cell counting kit-8 (CCK-8), flow cytometric, and wound healing assays in vitro.\nResults: We identified four clinical stage-related genes, GSR, AGPAT5, CRLF1, and NPR3, in CRC. The CNA frequencies of GSR, CRLF1, AGPAT5, and NPR3 occurred in 11%, 2.4%, 13%, and 3% of patients, respectively. The expression of GSR and AGPAT5 tended to decrease with CRC stage (I-IV) progression, and the expression of CRLF1 and NPR3 tended to increase with CRC stage (I-IV) progression. Compared with the normal group, AGPAT5 expression was markedly decreased in stage IV CRC. Higher GSR and AGPAT5 expression levels were associated with better overall survival (OS) and disease-free survival (DFS) in CRC patients. Lower CRLF1 and NPR3 expression levels were associated with better OS and DFS in CRC. GSR, CRLF1, AGPAT5, and NPR3 expression were related to CRC progression, microsatellite instability, and tumour purity in CRC. Furthermore, AGPAT5 was downregulated in CRC cell lines, and overexpression of AGPAT5 inhibited cell proliferation and migration and promoted cell apoptosis in CRC cells.\nConclusion: Low AGPAT5 expression may serve as a poor prognostic factor and clinical stage biomarker in CRC. In addition, AGPAT5 acts as a tumour suppressor in CRC progression.", "claims": [{"id": "claim-method", "statement": "The trend of genes related to clinical stage (I-IV) of CRC patients was identified by the JT test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36468151", "locator": "sections[16] \"Identification of clinical stage-related genes in TCGA COADREAD\" paragraph 2", "quote": "The trend of genes related to clinical stage (I-IV) of CRC patients was identified by the JT test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Transcriptome data and clinical information of CRC patients could be downloaded from the TCGA data portal (http://cancergenome.nih.gov/).The original contributions presented in the study are included in the article, further inquiries can be directed to the corresponding authors.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36468151", "locator": "sections[25] \"fn-group\" paragraph 1", "quote": "Transcriptome data and clinical information of CRC patients could be downloaded from the TCGA data portal (http://cancergenome.nih.gov/).The original contributions presented in the study are included in the article, further inquiries can be directed to the corresponding authors."}], "state": "complete"}, {"id": "claim-target-result", "statement": "We identified four clinical stage-related genes, GSR, AGPAT5, CRLF1, and NPR3, in CRC.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36468151", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "We identified four clinical stage-related genes, GSR, AGPAT5, CRLF1, and NPR3, in CRC."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "We identified four clinical stage-related genes, GSR, AGPAT5, CRLF1, and NPR3, in CRC.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36468151"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE41258"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE41258"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:36468151", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1177/11795549221137399", "sha256": "sha256:0408a048258ac53031e1800ce2cf33a632488f339c769c592ee7d843cfbdc6b0", "bytes": 33831, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1177/11795549221137399", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC9716453", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE41258", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE41258", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE41258", "version": null, "note": "Transcriptome data and clinical information of CRC patients could be downloaded from the TCGA data portal 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